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Preparation of Zein/Calcium Carbonate Composite Particles and Its Application as Drug Carrier

A technology of zein and composite particles, which is applied in the field of polymers and can solve problems such as limited application, lack of zein, and low nutritional value

Inactive Publication Date: 2016-01-13
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, zein lacks essential amino acids such as lysine and tryptophan, and its nutritional value is not high. In addition, its insolubility in water limits its application.

Method used

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  • Preparation of Zein/Calcium Carbonate Composite Particles and Its Application as Drug Carrier
  • Preparation of Zein/Calcium Carbonate Composite Particles and Its Application as Drug Carrier
  • Preparation of Zein/Calcium Carbonate Composite Particles and Its Application as Drug Carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of zein / calcium carbonate composite particles: fully disperse 10 mg zein in 1.5 mL of 70% ethanol solution, add 5.6 mg calcium chloride and stir to dissolve to form a mixture of calcium chloride and zein solution; then add the mixed solution to 95.5mL sodium carbonate solution (the mass of sodium carbonate is 5.3mg), and stir at a speed of 400r / min, so that the final concentration of zein is 0.1g . L -1 , the final mass concentration of the ethanol solution was 1%, and purified with a dialysis bag to obtain zein / calcium carbonate composite particles. The mass ratio of zein to calcium carbonate in the composite particles was 2:1, and the encapsulation efficiency and drug loading capacity of doxorubicin hydrochloride were 66.5% and 2.3%, respectively.

[0031] 2. Release test of doxorubicin hydrochloride: zein / calcium carbonate composite particles have good controlled release performance for doxorubicin hydrochloride in pH=7.4 buffer solution, the cumulati...

Embodiment 2

[0033] 1. Preparation of zein / calcium carbonate composite particles: fully disperse 200mg zein in 4.0mL75% ethanol solution, add 22.2mg calcium chloride and stir to dissolve to form a mixture of calcium chloride and zein solution; then add the mixed solution to 96.0mL sodium carbonate solution (sodium carbonate 21.2mg), stir at a speed of 500r / min, and control the final concentration of zein to 2g.L -1 , the final concentration of the ethanol solution was 3%, and purified with a dialysis bag to obtain zein / calcium carbonate composite particles. The mass ratio of zein to calcium carbonate in the composite particles was 10:1, and the encapsulation efficiency and drug loading capacity of doxorubicin hydrochloride were 68.2% and 2.4%, respectively.

[0034]2. Release performance of doxorubicin hydrochloride: doxorubicin hydrochloride has good controlled release performance in pH=7.4 buffer solution, the cumulative release curve is in a balanced state after 180hrs, and the cumulati...

Embodiment 3

[0036] 1. Preparation of zein / calcium carbonate composite particles: fully disperse 500mg zein in 11.3mL80% ethanol solution, add 18.5mg calcium chloride and stir to dissolve, forming a mixture of calcium chloride and zein solution; then the mixed solution was added to 88.7mL sodium carbonate solution (sodium carbonate 17.7mg), stirred at a speed of 600r / min, and the final concentration of zein was controlled to be 5g.L -1 , the final concentration of the ethanol solution was 9%, and purified with a dialysis bag to obtain zein / calcium carbonate composite particles. The mass ratio of zein to calcium carbonate in the composite particles was 30:1, and the encapsulation efficiency and drug loading capacity of doxorubicin hydrochloride were 69.7% and 2.6%, respectively.

[0037] 2. Release performance of doxorubicin hydrochloride: doxorubicin hydrochloride has good controlled release performance in pH=7.4 buffer solution, the cumulative release curve is in a balanced state after 20...

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Abstract

The invention discloses a preparation method of zein / calcium carbonate composite particles. The preparation method comprises the following steps of sufficiently dispersing the zein in 70%-90% alcohol liquor, adding calcium chloride and stirring and dissolving to form mixed liquor of calcium chloride and the zein, then, adding sodium carbonate liquor with amount of substance equal to that of calcium chloride, stirring at a high speed until the liquor is light blue; and then, keeping final concentration of the zein to 0.1g.L<-1> to 10 g.L<-1> by controlling addition amount of the zein, dialyzing and purifying to obtain the zein / calcium carbonate composite particles. Controlled release performance experiments of doxorubicin hydrochloride indicate that the zein / calcium carbonate composite particles have very good medicine carrying performance and controlled release performance to water-soluble small molecule drugs, and therefore, the zein / calcium carbonate composite particles can be used as water-soluble small molecule drug carriers for being used for producing pharmaceutical preparations.

Description

technical field [0001] The invention belongs to the technical field of macromolecules, and relates to the preparation of a zein / calcium carbonate composite particle; the invention also relates to the application of the zein / calcium carbonate composite particle as a drug carrier in the preparation of pharmaceutical preparations. Background technique [0002] Inorganic / polymer hybrid particles have unique advantages as drug carriers, including mild preparation conditions, no organic solvents and surfactants, and other favorable properties. Among them, calcium carbonate / polymer hybrid microspheres have good Due to its biocompatibility and biodegradability, this microsphere can be used in clinical treatment, and its porous internal structure endows it with the ability to simultaneously load hydrophilic drugs and hydrophobic drugs. In addition, calcium carbonate is pH-sensitive, and drug release can be triggered by the extracellular acid environment of solid tumor tissues and lys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/04A61K31/704
Inventor 何玉凤王燕裴菲王荣民徐知丽
Owner NORTHWEST NORMAL UNIVERSITY
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