69 results about "Reduce glutathione" patented technology

The invention discloses iron oxide nanoparticles capable of responding to particle size change in a tumor microenvironment and a preparation method of the iron oxide nanoparticles, and belongs to the technical field of biomedical nano materials. The nano-particle comprises an iron oxide nano-crystal core with the particle size of 3-5nm and a tumor microenvironment response layer coating the surface of the iron oxide nano-crystal core. The response layer contains groups, such as disulfide bonds, enzyme digestion peptide fragments and the like, which can respond to acidic pH, high-concentration reduced glutathione or matrix metalloproteinase and other tumor characteristic stimuli to generate structural changes. In a tumor microenvironment, the hydration particle size of the nanoparticles can be directionally and controllably converted from 3-20 nm to 20-100 nm (or reversely). The preparation method mainly comprises two steps of preparing the core by a thermal decomposition method and modifying the surface response layer. The technical problems of insufficient tumor targeted enrichment capacity, limited imaging contrast and difficulty in balancing permeation and retention caused by fixed particle size of the existing iron oxide nanoparticles are solved, and the accuracy and efficiency of tumor diagnosis and treatment can be remarkably improved.

The invention provides a high-entropy nano enzyme. The chemical formula of the high-entropy nano enzyme is Mn < 0.4 > Co < 0.4 > Ni < 0.4 > Cu < 0.4 > Zn < 0.4 > Fe < 2.0 >) O. The invention also provides a preparation method of the high-entropy nano-enzyme, which comprises the following steps: dissolving manganese nitrate tetrahydrate, cobalt nitrate hexahydrate, nickel nitrate hexahydrate, copper nitrate trihydrate, zinc nitrate hexahydrate and sodium citrate in deionized water to prepare a clear solution A; dissolving potassium ferricyanide in deionized water to prepare a clear solution B; directly pouring the solution B into the solution A, stirring, standing and aging the obtained mixed solution at room temperature, and centrifugally collecting a product; and calcining the sample powder in a muffle furnace to obtain the high-entropy nano-enzyme. The invention further provides a catalyst which is composed of the high-entropy nano enzyme, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2. The invention also provides a method for detecting reduced glutathione by using the microfluidic paper chip analysis device. The invention provides a new normal form for constructing a multifunctional biosensing and environment monitoring platform.

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

A method of measuring oxidative stress in a cell or tissue sample, the method comprising the steps of: (i) treating the cell or tissue sample in a culture medium; (ii) collecting a portion of the culture medium; (iii) measuring the amount of reduced glutathione (GSH) and oxidized glutathione (GSSG) in the cell or tissue sample; and (iv) measuring the amount of reactive oxygen species (ROS) in the culture medium portion collected in step (ii); wherein step (iii) is performed immediately after steps (i) and (ii); and wherein step (iv) is performed concurrently with step (iii), or within 6 hours after step (iii) is completed if the collected medium portion is stored at about 4 DEG C prior to performing step (iv), including the determination time.

The present invention discloses a formulation for alleviating plasticizer stress in crops and its application, belonging to the technical field of safe agricultural product production. This invention, for the first time, reveals the beneficial effects of reduced glutathione in alleviating plasticizer stress on crops. The use of reduced glutathione can significantly reduce the accumulation of plasticizers in crops, alleviating the inhibitory effects of plasticizers on crop growth. Furthermore, it proposes the application of reduced glutathione in crop production to effectively control and reduce plasticizer contamination of crops and avoid the adverse effects of plasticizers on the quality and safety of agricultural products.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention relates to the technical field of feed additives, in particular to a Chinese herbal medicine ultrafine powder feed additive and a preparation method thereof, the following raw materials are respectively weighed: codonopsis pilosula, acanthopanax, hawthorn, andrographis paniculata, citric acid, catechin, L-ascorbyl palmitate and reduced glutathione; immersing the codonopsis pilosula sections, the acanthopanax sections and the haw slices into enzymatic hydrolysate to carry out enzymatic hydrolysis reaction; crushing the codonopsis pilosula, the acanthopanax, the hawthorn and the andrographis paniculata sections subjected to enzymolysis by using a hammer crusher to obtain mixed powder; adding citric acid, catechin, L-ascorbyl palmitate and reduced glutathione, and mixing; the mixture is subjected to superfine grinding through a jet mill, and superfine powder is obtained; and mixing the superfine powder with hydroxypropyl-beta-cyclodextrin, adding water to prepare slurry, stirring and clathrating, and then carrying out spray drying to obtain the Chinese herbal medicine superfine powder feed additive.

The present invention provides an improved method for culturing mesenchymal stem cells and a culture medium used therefor, belonging to the technical field of cell culture. The improved mesenchymal stem cell culture medium provided by the present invention comprises the following exogenous additive components: recombinant human serum albumin, recombinant transferrin, recombinant human epidermal growth factor, basic fibroblast growth factor, platelet-derived growth factor, insulin, L-glutamine, reduced glutathione, β-mercaptoethanol, sodium selenite, penicillin-streptomycin, allicin, heparin and a proliferation promoter; the proliferation promoter comprises paeoniflorin, total saponins of Panax notoginseng, wolfberry polysaccharide and alcohol extract of Saussurea involucrata. By using the above culture medium for culturing mesenchymal stem cells, the cell activity and proliferation ability of mesenchymal stem cells can be significantly increased, the cell viability is increased by more than 50%, and the cell proliferation ability is increased by more than 40%, having great application potential in the culture of mesenchymal stem cells.

The invention provides a cascade response self-assembly polypeptide for remodeling tumor cell antigen composition, a bioactive solution of the cascade response self-assembly polypeptide and application of the cascade response self-assembly polypeptide. The polypeptide sequentially comprises a hydrophobic end-capping group, an alkaline phosphatase response self-assembly polypeptide sequence, a reduced glutathione response sequence and a T cell epitope peptide sequence. The polypeptide can respond to high-expression alkaline phosphatase in a tumor microenvironment to generate self-assembly and promote efficient internalization of cells; then, the antigen peptide is released under the action of reductive glutathione in tumor cells, and the antigen complex is given to the tumor cells through a main histocompatibility complex I-type molecular antigen presentation pathway. In addition, the specific hydrophobic end-capping group can up-regulate expression of I-type molecules of main histocompatibility complexes of tumor cells, enhance antigen presentation and remarkably enhance the recognition and killing efficiency of antigen-specific T cells on the tumor cells. Combined adoptive immunity and immune checkpoint inhibitor therapy is suitable for combined immunotherapy of solid tumors.

The application discloses a molecular probe and a preparation method and application thereof, and relates to the technical field of fluorescent probes.The structural formula of the molecular probe is as follows: the application takes two identical corrole units covalently connected as a parent body, and simultaneously introduces a reduced glutathione response group into the two corrole units, so that a molecular probe, namely a double-corrole molecular probe, which can remove reduced glutathione and realize NIR-II fluorescent signal opening, is constructed.The molecular probe can induce cell ferroptosis by consuming reduced glutathione in tumor cells.In addition, the molecular probe itself has a high photothermal conversion efficiency, and can perform photothermal treatment on tumor cells.Therefore, the molecular probe provided by the application is a companion diagnostic reagent integrating NIR-II fluorescent response, ferroptosis induction and photothermal treatment, has excellent antitumor effect, and has a wide application prospect.

The present invention provides nitrogen-, silicon-, and sulfur-codoped graphene quantum dots (GQDs) prepared by hydrothermal synthesis from waste carbon powder and reduced glutathione, wherein the mass ratio of waste carbon powder to reduced glutathione is 2:(4.5-5.5). The prepared GQDs exhibit high quantum yield, excellent stability, good water solubility, and good fluorescence properties. A N,Si,S-GQDs / OPD ratiometric fluorescent probe for autocatalytic rapid visual detection of Ag(I) and Fe(III) has been established. OPD is rapidly oxidized by Ag(I) / Fe(Ⅲ) to generate 2,3-diaminophenazine (oxOPD). Due to the inner filter effect between oxOPD and N,Si,S-GQDs, the fluorescence of N,Si,S-GQDs is significantly quenched. With the increase of Ag(I) / Fe(Ⅲ) concentration, the fluorescence of N,Si,S-GQDs at 440nm gradually weakens, while the fluorescence of oxOPD at 560nm gradually increases. Under ultraviolet light, the color of the solution gradually changes from blue to orange-yellow, enabling semi-quantitative visual detection.

The invention relates to oral probiotic hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing inulin-modified thiolated polysaccharide with a probiotic liquid, and quickly crosslinking to form probiotic hydrogel, so as to construct a colon-targeted probiotic delivery system; the system can effectively protect the probiotics from passing through the upper digestive tract, and realizes targeted release of the probiotics at the colon part by using a dual mechanism that the inulin is specifically ingested by the probiotics in the colon and the disulfide bond is easily reduced and disassembled by reduced glutathione (GSH) in the colon. The oral probiotic hydrogel disclosed by the invention is applied to preparation of medicines for preventing and treating irritable bowel syndrome, diarrhea, constipation, dyspepsia, inflammatory bowel disease, colon cancer, diabetes mellitus, obesity and hypercholesteremia.

The application discloses a kind of photonic nitric oxide donor with fluorescent self-reporting function and its preparation method and application, belong to the field of biomedical materials.The nitric oxide donor has the structure as shown in formula 1.The donor itself weak fluorescence, but in the presence of reduced glutathione (GSH), after irradiation by ultraviolet light (such as 405 nm), it can efficiently and controllably release nitric oxide (NO), and it is accompanied by strong fluorescence enhancement signal (fluorescence intensity can be increased by about 100 times), so as to realize the real-time, high-resolution fluorescence monitoring of NO release process and site.The donor has good light stability and biocompatibility.Experiments show that it can realize the light-controlled release and imaging of NO in living cells, and has application potential in the field of biomedical research and related drug development.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof, 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention provides application of an environmental response type anti-oxidation system to regulation and control of mitochondrial homeostasis and heart regeneration. The present invention relates to a reduced glutathione-modified polydopamine melanin nanoparticle, and particularly provides a reduced glutathione-modified polydopamine melanin nanoparticle, a method for promoting transformation of M1 macrophages to M2 macrophages by using the reduced glutathione-modified polydopamine melanin nanoparticle, a method for promoting damage repair of target cells, and a pharmaceutical composition.

The invention belongs to the technical field of antioxidant drugs, and particularly relates to application of a glutathione dry powder inhaler in preparation of drugs for treating radiation acute lung injury, the glutathione dry powder inhaler comprises the following active ingredients by mass percentage: 50%-60% of reduced glutathione, 10%-20% of a carrier and 20%-30% of a dispersant, totaling 100%, the carrier is mannitol, and the dispersing agent is L-leucine. According to the invention, the glutathione dry powder inhalant accurately delivers high-concentration GSH to an alveolar injury area through a lung targeting delivery system, synchronously activates endogenous antioxidant enzymes, inhibits lipid peroxidation and an inflammation source, and blocks release of proinflammatory factors, so that radiation-induced oxidation-inflammation cascade reaction is directly inhibited, alveolar structure damage is relieved, and the anti-inflammatory effect is achieved. And an efficient new treatment strategy is provided for RILI.

The application provides a tranexamic acid-glutathione compound cream and a preparation method thereof, adopts a W1 / O / W double emulsion phase structure design, encapsulates reduced glutathione in an acidic inner water core, stabilizes an oil phase by using hydrogenated lecithin and sorbitan sesquioleate, and constructs a lamellar gel network in an outer water phase containing tranexamic acid, so as to realize a sub-micron dispersion system with a particle size D50 of 0.30-0.80 microns, a polydispersity index of 0.15-0.25 and a viscosity of 2.0-8.0 Pa seconds, the encapsulation rate of glutathione is not less than 70%, and the headspace oxygen content is not higher than 1.0%, three technical contradictions of a high yield viscoelastic structure and a low shear damage inner core integrity coupling conflict, a smooth low skin feeling and a high barrier anti-oxidation and anti-leakage coexistence problem and a low surface activity sensitive skin compatibility and a sub-micron low PDI dispersion stability are solved, and the application has wide application values of improving uneven skin color, lightening color spots, brightening skin color and soothing and repairing.

The invention discloses an application of a compound gel preparation in preparation of a product for treating chloasma. The compound gel disclosed by the invention is prepared from polypeptide gel capable of opening tight connection of skin through a self-assembly method. Finally, the hydrogel with certain flowability is formed. The polypeptide gel formed by the invention can penetrate through the surface layer of the skin, tranexamic acid, vitamin C and reduced glutathione are transferred to the basal layer of the skin through one-time operation, and then tranexamic acid is competitively combined with tyrosinase to inhibit the activity of the tyrosinase, so that the formation of melanin is reduced; the vitamin C inhibits the formation of melanin by reducing the formation of o-quinone and reducing oxidized melanin; the reduced glutathione is used as an antioxidant component, can inhibit the generation of free radicals, helps to stabilize the skin and restore the skin whiteness, can be used for treating chloasma, and provides a new solution for the field of skin health treatment.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The invention discloses a composition for protecting liver functions and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations. The preparation method comprises the following steps: firstly, treating chrysanthemum in steps, preparing part of chrysanthemum into sterilized fine powder for later use, and adding part of chrysanthemum into an extraction system; the preparation method comprises the following steps: crushing and breaking shells of part of cassia seed and cistanche, mixing with Chinese angelica, performing ethanol percolation extraction, performing secondary water reflux extraction on decoction dregs and other traditional Chinese medicine raw materials, combining percolate and reflux filtrate, and concentrating; synchronously preparing a silybum marianum extract, a Chinese magnoliavine fruit extract and reduced glutathione which meet standards, spray-drying a concentrated solution, adding the chrysanthemum fine powder and the three types of core extracts, and performing three-dimensional mixing to obtain a finished product. The silymarin is used for enhancing liver cell membrane protection, the schisandra chinensis extract is used for activating an endogenous antioxidant system, the reduced glutathione is used for removing toxic substances, the traditional Chinese medicine components are used for synergistic interaction, meanwhile, the process is optimized to guarantee uniform component retention and distribution, and the problems that a traditional preparation is single in effect and unstable in quality are solved.

The application discloses a creatine kinase determination kit and a use method thereof. The creatine kinase determination kit comprises an R1 component and an R2 component. The R1 component comprises oxidized glutathione and auxiliary material 1. The R2 component comprises reduced coenzyme II, glucose-6-phosphate dehydrogenase, creatine phosphate, glutathione reductase and auxiliary material 2. The oxidized glutathione (GSSG) is reduced into reduced glutathione (GSH) by catalytic degradation of glutathione reductase (GR), and the reduced glutathione contains -SH, thereby activating CK. By the above method, the -SH compound N-acetyl-L-cysteine does not need to be added, thereby fundamentally solving the problem that -SH is gradually oxidized and deactivated, greatly improving the long-term stability of the reagent, and prolonging the effective period of the reagent.

The invention discloses a multi-component synergistic selenium-containing antibacterial system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. According to the method, water is adopted as a preparation medium, sodium selenite and a surfactant are added into the water, and reduced glutathione is used for reduction, so that nano-selenium colloid is prepared; subsequently adding an ammonium salt regulator and an alcohol regulator, and stirring to obtain the selenium-containing antibacterial system. The obtained selenium-containing antibacterial system has good broad-spectrum antibacterial activity.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention belongs to the field of hydrogel, and particularly discloses drug sustained-release polypeptide hydrogel and a preparation method thereof, the drug sustained-release polypeptide hydrogel comprises a polypeptide polymer, a cross-linking agent, a drug and a solvent; a main chain of the polypeptide polymer is a glycine-alanine-serine repetitive unit, and side chains of the polypeptide polymer are grafted with carboxyl and sulfydryl bifunctional groups; the cross-linking agent is prepared from oxidized hyaluronic acid and thiolated chitosan; the solvent is a phosphate buffer solution containing 5 to 10 mM of reduced glutathione; according to the preparation method, a pH, reduction and enzyme triple responsive cross-linked network is constructed, a surface large-aperture-internal small-aperture gradient pore structure is designed, hyaluronic acid, chitosan, polypeptide and other natural polymer base materials are adopted, and HA-CD44 targeting peptide modification is introduced, so that the hydrogel is prepared. Accurate trigger release of the drug in a pathological microenvironment, a two-phase slow release mode, biocompatibility improvement of non-toxic degradation products, and active targeting ability of increase of drug enrichment amount at a tumor site are correspondingly realized.

The invention belongs to the technical field of gene editing, and particularly relates to an efficient grouper gene editing method based on fertilized egg injection. The method comprises the following steps: soaking fertilized eggs of groupers fertilized within 10 minutes in a weakening chorion culture medium buffer solution at 26 DEG C for 30-60 minutes, then taking out the fertilized eggs, and injecting a target CRISPR / Cas9 mixture into the fertilized eggs of the groupers before embryos develop to a four-cell stage; the attenuated chorion culture medium buffer solution is prepared by using sterilized seawater and comprises 102.8 mM of sodium chloride, 10mM of trihydroxymethyl aminomethane, 20mM of glycine and 1mM of reduced glutathione. According to the application, the rapid hardening of the chorion of the fertilized egg of the grouper can be effectively weakened, after the hardening time is prolonged to a four-cell stage, a relatively long operation window stage is obtained for micro-injection, the injection efficiency and the injection success rate can be improved, and the injection problem of the fertilized egg of the grouper is solved.

The invention relates to the technical field of biological monitoring, in particular to a preparation method and application of a blood plate. The preparation method of the blood plate comprises the following steps: adding culture medium components into a culture medium, the culture medium components comprising peptone nitrogen, a yeast extract, reduced glutathione, sodium pyruvate and distilled water; adding blood into the culture medium; adjusting the pH value of the culture medium to be stable; adding a heat-sensitive component and a heat-stable component into the culture medium; and constructing a culture medium low-oxygen mixing environment. Therefore, the shelf life of the blood plate can be prolonged, and the hemolysis phenomenon is reduced.

The invention discloses a method suitable for large-scale production of recombinant conopeptide. The conopeptide fusion protein containing the HIS tag-TrxA tag protein-enterokinase recognition site-conopeptide is constructed, a redox pair (reduced glutathione / oxidized glutathione) is introduced in vitro in the separation process, a redox reaction system is optimized, the mismatch rate of the recombinant conopeptide can be remarkably reduced, and the yield of the recombinant conopeptide is increased by more than one time. In addition, the enzyme digestion system is further optimized, and the recombinant conopeptide with high purity and correct structure is finally obtained. The invention provides an effective solution for large-scale production of conopeptide, and has huge application value.

The invention provides a method for purifying reduced glutathione, which comprises the following steps: preparing a glutathione solution to obtain a saturated glutathione solution; titrating a crystallization agent into the saturated glutathione solution, and continuously stirring at a constant speed for a second preset duration to obtain a pre-crystallized glutathione solution; and standing the pre-crystallized glutathione solution until glutathione crystals are separated out, and keeping the glutathione solution in a constant temperature T state in the above processes, T being 30-40 DEG C. Crude glutathione is dissolved at low temperature and constant temperature, so that reduced glutathione is prevented from being oxidized, and the utilization rate of glutathione is guaranteed; by matching the additive solution with the crystallization agent, the purity and the appearance of the recrystallized glutathione are improved, the whole reaction process is mild, the pH value, the temperature range, the pressure and the like of the solution do not need to be adjusted, operation is easy, industrial production is facilitated, and the concept of energy conservation, environmental protection and greenness is met.

The invention discloses a compound oral film and a preparation method thereof, the compound oral film comprises functional components and preparation components, the functional components comprise 45-55 parts of reduced glutathione GSH, 10-12 parts of nicotinamide, 5-15 parts of pyrroloquinoline quinone PQQ, 5-15 parts of a white tomato extract, and 4-6 parts of vitamin C; the preparation comprises the following components in parts by weight: 85-90 parts of a thickening agent, 5-15 parts of an emulsifying agent, 30-35 parts of a sweetening agent and 4.15 parts of other acceptable auxiliaries. The reduced glutathione directly plays an anti-oxidation role, the PQQ also can enhance the anti-oxidation circulation of the GSH and has a synergistic effect to enhance the whitening effect, and meanwhile, the vitamin C reduces the loss of the PQQ due to oxidation in blood circulation; the PQQ has high biological activity, can play a physiological role in a low dosage, is suitable for a supply mode of the oral film, and has high efficiency of realizing a skin whitening function.

The application discloses gold nanoclusters selectively combined with G-quadruplexes, a preparation method and application thereof, and the preparation method comprises the following steps: synthesizing gold nanoclusters by means of a hydrothermal reaction of reduced glutathione, N, N, N-trimethyl-(11-mercapto undecyl) ammonium chloride and chloroauric acid, wherein the preparation method is simple, the prepared gold nanoclusters are water-soluble gold nanoclusters capable of being specifically combined with G-quadruplexes, can easily enter into cell nuclei to be combined with G-quadruplex DNA, and exhibit an anti-tumor effect.