319 results about "Reduce glutathione" patented technology

The invention discloses a method for preparing a coating with nitric oxide catalytic activity. The method comprises the following steps: mixing a selenium-containing compound, a sulfur-containing compound and a soluble copper salt with the NO catalytic activity with a compound of an o-phenol structure, a flavone compound and a flavonol compound or a flavanone compound in a buffer solution for polymerizing. The coating with the nitric oxide catalytic activity can be applied to surface modification of matrix materials of almost all the materials, geometrical shapes and topological structures. The double selenium bonds, double sulfur bonds, copper ions and phenolic hydroxyl group in the prepared coating with the nitric oxide (NO) catalytic activity have excellent free radical scavenging functions; and the selenium bonds, sulfur bonds and chelated copper ions contained in the material have the response functions on reduced glutathione (GSH). In addition, the copper ions contained in the coating have antibacterial functions. The coating is used for catalyzing NO release and also can be applied to all the fields related to free radical scavenging and GSH response functions.

The invention relates to a fluorescent probe, wherein, the fluorescence is enhanced in the presence of ONOO-, and the fluorescence returns to original state in the presence of reducing matter. The invention provides a reversible fluorescent probe for selective detection of ONOO- in cells, characterized in that: cyanine dye is used as the fluorescent matrix, an organic selenide structure is introduced in the cyanine matrix as the active site of the reaction with ONOO- to realize the selective detection of ONOO-; the properties that organic selenium oxide formed by oxidizing organic selenide is easy to be reduced to organic selenide by reducing micromolecules in biosystem, such as cysteine, reducing glutathione, metallothionein and the like, is utilized to realize the reversibility of the probe molecules; and variations of electronic properties of organic selenide before and after the oxidation and the influence of the variations on the fluorescence property of the whole compound are utilized simultaneously to modulate the fluorescence property of the probe molecules.

A composition that is used as an eye treatment contains reduced glutathione, vitamin A and vitamin E, as well as one or more of zinc sulfate, boric acid and potassium as buffering agents. The composition also may contain a lubricant and a preservative. The composition is a sterile isotonic solution. The composition is used in a method of treating eyes for the alleviation of irritations and/or dryness, as well as for the prevention and treatment of cataracts.

The present invention relates to a method for analyzing residual agricultural chemicals which comprises the steps of acting a reduced glutathione as a reactive substrate and a glutathione transferase serving as a catalyst for the reaction on a carbofuran derivative or a methomyl derivative as a carbamate type agricultural chemical of a new series to thus derivatize the agricultural chemical into a substance having a high choline esterase-inhibitory activity; reacting the substances formed through the derivatization reaction with a choline esterase; and then detecting the presence of the agricultural chemical as the new series of carbamate type one included in a sample to be examined on the basis of the changes in the choline esterase activity thus detected. The method of the present invention may serve as a powerful tool for the detection of the residual agricultural chemicals in grains such as rice and the detection of the content of agricultural chemicals remaining in agricultural products such as vegetables and fruits.

The invention provides preserving fluid of hepatic cells for a biological artificial liver and a preparation method thereof. The preserving fluid is a solution compounded by ultrapure water. The solution contains the following components within the concentration range: 15-25mmol/L of disodium hydrogen phosphate, 1-10mmol/L of sodium hydrogen phosphate dehydrate, 4-6mmol/L of potassium citrate monohydrate, 10-30mmol/L of sodium chloride, 5-10mmol/L of magnesium chloride hexahydrate, 3-10mmol/L of disodium adenosine triphosphate, 1-5mmol/L of reducing glutathione, 0.1-0.5mmol/L of alpha-lipoic acid, 100-150mmol/L of trehalose (C6H12O5), 200/0.510-50g/L of hydroxyethyl starch and 2-10mg/L of matrine. The preparation method of the preserving fluid comprises the following steps of: accurately weighing all components according to the concentration requirements of the components, wherein the alpha-lipoic acid is weighed in a dark place; completely dissolving the other components except the alpha-lipoic acid by using the right amount of ultrapure water; sufficiently dissolving the alpha-lipoic acid in the dark place; and adding the ultrapure water to full dose. The preserving fluid can well protect the cell activity of the hepatic cells for the biological artificial liver and the special functions of the hepatic cells at low temperature so as to satisfy the short-term low temperature preservation of a large-scale hepatic cell bank for the biological artificial liver and/or the hepatic cell protection in the long-distance transportation process.

Production of injection reducing glutathione is carried out by pre-freezing for reducing glutathione solution and freeze-drying. It's simple, cheap, has stable product quality and less water content and related substances and can be used for industrial production.

The invention relates to an organ preservation solution and a method for preparing the same. The method comprises the following steps of: (1) adopting double buffer pairs of citric acids and phosphates to enable the PH value of a solution to be maintained in a range of 7.5 to 8; (2) adding active ingredients such as adenosine, L-arginine, tryptophan, ligustrazine hydrochloride, reduced glutathione and the like and keep the solution stable; (3) to maintain a hypertonic property of the preservation solution with an osmotic pressure of 350-380 mOsm/L, adopting trehalose and gluconolactone as impermeable substances to effectively prevent cellular edema; and (4) adopting polyethylene glycol with a large molecular weight as a colloidal substance to effectively prevent extracellular space from expanding. Compared with the prior art, the method employs raw materials which are all homemade medicinal components and meet the standards of national pharmacopoeia, and are cheap and free of incompatibility; the organ preservation solution prepared is a colorless and transparent liquid which has stable properties, and can be sterilized by high temperature and high pressure, stored at normal temperature or low temperature, and refrigerated, and thus preparation, transportation, storage and usage of the organ preservation liquid are all very easy and convenient.

The invention provides a production method for cordyceps militaris buccal tablets with a high practical value and a good combination effect and belongs to industrial production. The technology adopted by the preparation method is characterized in that the content of active components such as reduced glutathione and a cordyceps militaris extract can be controlled, so that the aim of realizing stable quality can be fulfilled.

The invention provides a preparation method of pH and reduction sensitive nano microgel based on polyglutamic acid and cystamine and application of the pH and reduction sensitive nano microgel based on polyglutamic acid and cystamine in the anti-tumor aspect. The nano microgel with a particle size of 212nm is synthesized by preparing a microgel model from raw materials including rolyglutamic acid and cystamine under electrostatic interaction, and adding a catalyst to carry out crosslinking amidation reaction. Because carboxylate anions in polyglutamic acid are combined with amino cations in doxorubicin to load medicines, a high medicine lading ratio of 17.8% can be achieved. The disulfide bond in cystamine which serves as a crosslinking agent ensures that nano particles are sensitive to reduction, and the disulfide bond can break in a cancer cell containing high-concentration reduced glutathione, and thus the nano microgel is targeted at tumors and has a controlled release function. The nano microgel is directly prepared in an aqueous solution without adopting any organic solvent, and thus the safety of the medicine carrier can be guaranteed.

The invention discloses a prefreezing method in preparing injection-used reduced glutathione with a freeze drying method, comprising the following steps: (1) quantitatively split charging reduced glutathione solution into each tube-type bottle according to the specification of a final product, lowering the temperature below -40 DEG C in 1.5-2 hours, and keeping the temperature for 1 hour to obtaina reduced glutathione solution freezing body; (2) rising the temperature of the reduced glutathione solution freezing body obtained in step (1) to -17 DEG C in two hours, and keeping the temperaturefor one hour; (3) lowering the temperature of the reduced glutathione solution freezing body obtained in step (2) below -40 DEG C within 1-1.5 hours, and keeping the temperature for one hour. After the prefreezing method of the invention is adopted, the obtained substance is sublimated and dried to obtain a white loose block product, and surface has no appearance defect. The product obtained by the prefreezing method of the invention has small possibility of erupting in the production process, and the quality of the final product conforms to the national standard.

The invention is the use of a therapeutically effective amount of glutathione (reduced) in a liposome encapsulation for oral administration to improve symptoms of illnesses that are related to viruses and for the treatment and prevention of virus, particularly HHV-6 and EBV, which liposomal encapsulation of glutathione (reduced) is referred to as liposomal glutathione. The application references specifically reduced glutathione and its importance, and how to stabilize it effectively so it can be taken orally, and need not be refrigerated. New uses for tuberculosis, and asthma are discussed. The combination is proposed of reduced glutathione and Highly Active Anti-Retroviral Therapy having at least one pharmaceutical composition selected from the group of Nucleoside/tide Reverse Transcriptase Inhibitors (NRTIs), Protease Inhibitors (PIs), and Non-nucleoside Reverse Transcriptase Inhibitors (NnRTIs).

The invention discloses a preparation method of a material with nitric oxide (NO) catalytic activity. The preparation method is implemented through the steps that a selenium-containing compound with nitric oxide catalytic activity, a sulfur-containing compound, a soluble copper salt, a compound with an o-phenol structure, a flavonoid compound, and a flavonol compound or a flavanone compound are mixed in a buffer solution and then polymerized. The material with NO catalytic activity not only can be applied to the surface modification of materials which are different in material, geometrical shape and topological structure, and also can be used as a filling material for a controlled release system. Double selenium bonds, double sulfur bonds, copper ions and phenolic hydroxyl groups, which are contained in the prepared material with nitric oxide (NO) catalytic activity, have an excellent free radical removal function; the selenium bonds, the sulfur bonds and the chelate copper ions, which are contained in the material, also have a reduced glutathione (GSH) response function; in addition, the copper ions containing in the material also have an antibacterial function; and the material, besides being used for catalyzing NO release, also can be applied to all related fields of free radical removal and GSH response function.

The invention belongs to the field of preparation of biological medical materials, and relates to a method for preparing stable albumin nano-particles by virtue of thermal denaturation. The method comprises the following steps: (1) adding vanillic aldehyde or an analogue thereof to form intermolecular disulfide bonds by virtue of inter-reaction of free sulfhydryl groups on albumin molecules under a heating condition; (2) enabling amino groups inside and among molecules to react with carboxyl so as to form amido bonds; and (3) enabling amino groups on the albumin molecules to react with aldehyde groups on vanillic aldehyde or the analogue thereof to form chemical bonds of Schiff base and the like so as to form stable nano-particles in an aqueous solution. Any organic solvent is not introduced during preparation, so that the prepared nano-particles are safe and nontoxic, and can well entrap antitumor drugs including paclitaxel, doxorubicin hydrochloride and the like. Moreover, the carrier has an oxidation reduction response in a tumor cell internal environment, and can open disulfide bonds to release drugs under the action of reducing glutathione in cells. The method provided by the invention is simple in process, convenient to operate and suitable for industrial mass production.

The invention is the use of a therapeutically effective amount of glutathione (reduced) in a liposome encapsulation for oral administration to improve symptoms of illnesses that are related to tuberculosis and HIV and more generally viruses and for the treatment and prevention of virus, particularly HHV-6 and EBV, which liposomal encapsulation of glutathione (reduced) is referred to as liposomal glutathione. The application references specifically reduced glutathione and its importance, and how to stabilize it effectively so it can be taken orally, and need not be refrigerated. New uses for tuberculosis are discussed. The combination is proposed of reduced glutathione and Highly Active Anti-Retroviral Therapy having at least one pharmaceutical composition selected from the group of Nucleoside/tide Reverse Transcriptase Inhibitors (NRTIs), Protease Inhibitors (PIs), and Non-nucleoside Reverse Transcriptase Inhibitors (NnRTIs), and further anti-tuberculosis drugs.

The present invention is directed to a method for decreasing inflammation and oxidative stress in a mammal comprising; administration to a mammal a composition comprising a glucose anti-metabolite; and wherein said composition comprises amounts of the glucose anti-metabolite sufficient to decrease a level of an oxidized glutathione and/or increase the ration of reduced glutathione to oxidized glutathione in the blood of the mammal subsequent to administration of the glucose anti-metabolite.

Methods for the prevention and treatment of skin damage arising from the exposure to UV radiation, air pollution and other stressors capable of leading to the formation of free radicals, using the small-dithiol protein glutaredoxin in a dermatologically acceptable carrier that can be applied topically are disclosed. Some embodiments may include other proteins such as superoxide dismutase and/or catalase, as well as other antioxidant molecules such as reduced glutathione, vitamin E, vitamin C, lycopene, astaxanthin and/or tocotrienols.

The invention discloses acipenser dabryanus sperm preserving fluid liquid and a preparing method and application. The acipenser dabryanus sperm preserving fluid liquid comprises saccharose, mycose, trihydroxy aminomethane, potassium chloride, reduced glutathione and carbinol. The acipenser dabryanus sperm preserving fluid liquid has simple components and is low in cryopreservation cost. The added reduced glutathione plays a good role in antioxygenation, well protects sperm plasma membranes and reduces damage to DNA molecules. The added mycose has the effects of preventing protein denaturation and effectively protecting sperms. By means of the acipenser dabryanus sperm preserving fluid liquid, motility of unfreezed sperms is higher than 70%, and the fertility rate reaches 45%.

The invention discloses a preparation method for a Cu3BiS3 micro/nanosheet. The preparation method comprises the following steps: 1, dissolving a copper-containing precursor, a bismuth-containing precursor, a sulfur-containing precursor and a chelating agent in water to prepare a solution, wherein the copper-containing precursor is copper nitrate, copper sulfate or copper acetate, the bismuth-containing precursor is bismuth nitrate or bismuth acetate, the sulfur-containing precursor is thiourea, thioacetamide, sublimed sulfur or sodium sulfate, and the chelating agent is reduced glutathione; 2, heating the solution prepared in the step 1, producing a reaction completely, cooling to a room temperature, performing centrifugal treatment, washing sediments obtained by centrifuging with water and ethanol, drying, and preparing the Cu3BiS3 micro/nanosheet. The preparation method is simple in process, adopts low-price copper source, bismuth source and sulfur source as the precursors, uses solid-state nontoxic reduced glutathione as the chelating agent and uses the water as a solvent, so that the preparation method is low in cost and favorable for popularization and application.

The invention discloses a reduction trigger type polypeptide modified hyaluronic acid conjugate carrier and a preparation method thereof. The carrier is a nanoparticle mainly consisting of hyaluronic acid, hydrophobic groups, tumor targeted hydrophilic groups and a medicament, wherein the hydrophobic groups are bonded with hyaluronic acid through reduction sensitive bonds. The conjugate carrier can be self-assembled into the nanoparticle in an aqueous medium and is capable of supporting anti-tumor active medicine molecules. The reduction trigger type polypeptide modified hyaluronic acid conjugate carrier has the main advantages that (1) the carrier has a dual targeting capacity, so that the transfer efficiency of drugs in tumors is improved, and the untoward effect is reduced; (2) a disulfide bond linking arm is intruded into the conjugate and can be specifically degraded by high-concentration reduced glutathione in the tumor cells, so that the drugs are rapidly released, and the bioavailability of the drugs is improved; and (3) the anti-tumor drugs are loaded by virtue of a physical embedding action, so that the water solubility of the anti-tumor drugs is improved. The preparation method is simple, a process is mature, and the carrier has a good application prospect.

The invention discloses autologous-repair nutrient injection. The injection comprises the following main components: collagen and physiological saline. By further improvement, the injection can further comprise lidocaine, reduced glutathione, vitamin C, beta thymosin, and hyaluronic acid with low molecular weight. The invention solves the problems that absorbable injectants have no lasting effectand need repeated injection, and also solves the problem that the non-absorbable injectants have potential safety hazards. The injection completely performs autologous repair on the growing skin, causes no rejection and allergy, and has long timeliness and natural effect. The injection is most suitable for people who pursue safe and natural beautifying, people who seek to recover within a short period without influencing daily work and life, and people who seek to maintain their own characteristics with exquisite beautifying.

The invention discloses a separation and cultivation method of human adipose stem cells. The method comprises the following steps: first, digesting a fat suction matter by using a Liberase digestive enzyme solution, neutralizing digestive enzyme after digestion is ended, centrifuging, filtering, and further removing parenchyma cells by using a lymphocyte separation solution so as to obtain the human adipose stem cells; second, cultivating the human adipose stem cells by using a serum-free medium, wherein following components are added in the serum-free medium: a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human transforming growth factor-beta, a recombinant human platelet-derived growth factor-BB, a recombinant human stem cell factor, reduced glutathione, coenzyme A, biotin, MEM vitamin solution, MEM amino acid solution, MEM non-essential amino acid solution, a GlutaMAX additive, insulin-transferrin-selenium solution andgentamicin. The method can remarkably increase the yield of adipose stem cells and improve the activity of the adipose stem cells, so as to obtain more adipose stem cells with proliferation ability.