51 results about "Reduce glutathione" patented technology

The invention discloses iron oxide nanoparticles capable of responding to particle size change in a tumor microenvironment and a preparation method of the iron oxide nanoparticles, and belongs to the technical field of biomedical nano materials. The nano-particle comprises an iron oxide nano-crystal core with the particle size of 3-5nm and a tumor microenvironment response layer coating the surface of the iron oxide nano-crystal core. The response layer contains groups, such as disulfide bonds, enzyme digestion peptide fragments and the like, which can respond to acidic pH, high-concentration reduced glutathione or matrix metalloproteinase and other tumor characteristic stimuli to generate structural changes. In a tumor microenvironment, the hydration particle size of the nanoparticles can be directionally and controllably converted from 3-20 nm to 20-100 nm (or reversely). The preparation method mainly comprises two steps of preparing the core by a thermal decomposition method and modifying the surface response layer. The technical problems of insufficient tumor targeted enrichment capacity, limited imaging contrast and difficulty in balancing permeation and retention caused by fixed particle size of the existing iron oxide nanoparticles are solved, and the accuracy and efficiency of tumor diagnosis and treatment can be remarkably improved.

The invention provides a high-entropy nano enzyme. The chemical formula of the high-entropy nano enzyme is Mn < 0.4 > Co < 0.4 > Ni < 0.4 > Cu < 0.4 > Zn < 0.4 > Fe < 2.0 >) O. The invention also provides a preparation method of the high-entropy nano-enzyme, which comprises the following steps: dissolving manganese nitrate tetrahydrate, cobalt nitrate hexahydrate, nickel nitrate hexahydrate, copper nitrate trihydrate, zinc nitrate hexahydrate and sodium citrate in deionized water to prepare a clear solution A; dissolving potassium ferricyanide in deionized water to prepare a clear solution B; directly pouring the solution B into the solution A, stirring, standing and aging the obtained mixed solution at room temperature, and centrifugally collecting a product; and calcining the sample powder in a muffle furnace to obtain the high-entropy nano-enzyme. The invention further provides a catalyst which is composed of the high-entropy nano enzyme, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2. The invention also provides a method for detecting reduced glutathione by using the microfluidic paper chip analysis device. The invention provides a new normal form for constructing a multifunctional biosensing and environment monitoring platform.

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

A method of measuring oxidative stress in a cell or tissue sample, the method comprising the steps of: (i) treating the cell or tissue sample in a culture medium; (ii) collecting a portion of the culture medium; (iii) measuring the amount of reduced glutathione (GSH) and oxidized glutathione (GSSG) in the cell or tissue sample; and (iv) measuring the amount of reactive oxygen species (ROS) in the culture medium portion collected in step (ii); wherein step (iii) is performed immediately after steps (i) and (ii); and wherein step (iv) is performed concurrently with step (iii), or within 6 hours after step (iii) is completed if the collected medium portion is stored at about 4 DEG C prior to performing step (iv), including the determination time.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention relates to the technical field of feed additives, in particular to a Chinese herbal medicine ultrafine powder feed additive and a preparation method thereof, the following raw materials are respectively weighed: codonopsis pilosula, acanthopanax, hawthorn, andrographis paniculata, citric acid, catechin, L-ascorbyl palmitate and reduced glutathione; immersing the codonopsis pilosula sections, the acanthopanax sections and the haw slices into enzymatic hydrolysate to carry out enzymatic hydrolysis reaction; crushing the codonopsis pilosula, the acanthopanax, the hawthorn and the andrographis paniculata sections subjected to enzymolysis by using a hammer crusher to obtain mixed powder; adding citric acid, catechin, L-ascorbyl palmitate and reduced glutathione, and mixing; the mixture is subjected to superfine grinding through a jet mill, and superfine powder is obtained; and mixing the superfine powder with hydroxypropyl-beta-cyclodextrin, adding water to prepare slurry, stirring and clathrating, and then carrying out spray drying to obtain the Chinese herbal medicine superfine powder feed additive.

The invention provides a cascade response self-assembly polypeptide for remodeling tumor cell antigen composition, a bioactive solution of the cascade response self-assembly polypeptide and application of the cascade response self-assembly polypeptide. The polypeptide sequentially comprises a hydrophobic end-capping group, an alkaline phosphatase response self-assembly polypeptide sequence, a reduced glutathione response sequence and a T cell epitope peptide sequence. The polypeptide can respond to high-expression alkaline phosphatase in a tumor microenvironment to generate self-assembly and promote efficient internalization of cells; then, the antigen peptide is released under the action of reductive glutathione in tumor cells, and the antigen complex is given to the tumor cells through a main histocompatibility complex I-type molecular antigen presentation pathway. In addition, the specific hydrophobic end-capping group can up-regulate expression of I-type molecules of main histocompatibility complexes of tumor cells, enhance antigen presentation and remarkably enhance the recognition and killing efficiency of antigen-specific T cells on the tumor cells. Combined adoptive immunity and immune checkpoint inhibitor therapy is suitable for combined immunotherapy of solid tumors.

The application discloses a molecular probe and a preparation method and application thereof, and relates to the technical field of fluorescent probes.The structural formula of the molecular probe is as follows: the application takes two identical corrole units covalently connected as a parent body, and simultaneously introduces a reduced glutathione response group into the two corrole units, so that a molecular probe, namely a double-corrole molecular probe, which can remove reduced glutathione and realize NIR-II fluorescent signal opening, is constructed.The molecular probe can induce cell ferroptosis by consuming reduced glutathione in tumor cells.In addition, the molecular probe itself has a high photothermal conversion efficiency, and can perform photothermal treatment on tumor cells.Therefore, the molecular probe provided by the application is a companion diagnostic reagent integrating NIR-II fluorescent response, ferroptosis induction and photothermal treatment, has excellent antitumor effect, and has a wide application prospect.

The invention relates to oral probiotic hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing inulin-modified thiolated polysaccharide with a probiotic liquid, and quickly crosslinking to form probiotic hydrogel, so as to construct a colon-targeted probiotic delivery system; the system can effectively protect the probiotics from passing through the upper digestive tract, and realizes targeted release of the probiotics at the colon part by using a dual mechanism that the inulin is specifically ingested by the probiotics in the colon and the disulfide bond is easily reduced and disassembled by reduced glutathione (GSH) in the colon. The oral probiotic hydrogel disclosed by the invention is applied to preparation of medicines for preventing and treating irritable bowel syndrome, diarrhea, constipation, dyspepsia, inflammatory bowel disease, colon cancer, diabetes mellitus, obesity and hypercholesteremia.

The application discloses a kind of photonic nitric oxide donor with fluorescent self-reporting function and its preparation method and application, belong to the field of biomedical materials.The nitric oxide donor has the structure as shown in formula 1.The donor itself weak fluorescence, but in the presence of reduced glutathione (GSH), after irradiation by ultraviolet light (such as 405 nm), it can efficiently and controllably release nitric oxide (NO), and it is accompanied by strong fluorescence enhancement signal (fluorescence intensity can be increased by about 100 times), so as to realize the real-time, high-resolution fluorescence monitoring of NO release process and site.The donor has good light stability and biocompatibility.Experiments show that it can realize the light-controlled release and imaging of NO in living cells, and has application potential in the field of biomedical research and related drug development.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof, 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention belongs to the technical field of antioxidant drugs, and particularly relates to application of a glutathione dry powder inhaler in preparation of drugs for treating radiation acute lung injury, the glutathione dry powder inhaler comprises the following active ingredients by mass percentage: 50%-60% of reduced glutathione, 10%-20% of a carrier and 20%-30% of a dispersant, totaling 100%, the carrier is mannitol, and the dispersing agent is L-leucine. According to the invention, the glutathione dry powder inhalant accurately delivers high-concentration GSH to an alveolar injury area through a lung targeting delivery system, synchronously activates endogenous antioxidant enzymes, inhibits lipid peroxidation and an inflammation source, and blocks release of proinflammatory factors, so that radiation-induced oxidation-inflammation cascade reaction is directly inhibited, alveolar structure damage is relieved, and the anti-inflammatory effect is achieved. And an efficient new treatment strategy is provided for RILI.

The application provides a tranexamic acid-glutathione compound cream and a preparation method thereof, adopts a W1 / O / W double emulsion phase structure design, encapsulates reduced glutathione in an acidic inner water core, stabilizes an oil phase by using hydrogenated lecithin and sorbitan sesquioleate, and constructs a lamellar gel network in an outer water phase containing tranexamic acid, so as to realize a sub-micron dispersion system with a particle size D50 of 0.30-0.80 microns, a polydispersity index of 0.15-0.25 and a viscosity of 2.0-8.0 Pa seconds, the encapsulation rate of glutathione is not less than 70%, and the headspace oxygen content is not higher than 1.0%, three technical contradictions of a high yield viscoelastic structure and a low shear damage inner core integrity coupling conflict, a smooth low skin feeling and a high barrier anti-oxidation and anti-leakage coexistence problem and a low surface activity sensitive skin compatibility and a sub-micron low PDI dispersion stability are solved, and the application has wide application values of improving uneven skin color, lightening color spots, brightening skin color and soothing and repairing.

The invention discloses an application of a compound gel preparation in preparation of a product for treating chloasma. The compound gel disclosed by the invention is prepared from polypeptide gel capable of opening tight connection of skin through a self-assembly method. Finally, the hydrogel with certain flowability is formed. The polypeptide gel formed by the invention can penetrate through the surface layer of the skin, tranexamic acid, vitamin C and reduced glutathione are transferred to the basal layer of the skin through one-time operation, and then tranexamic acid is competitively combined with tyrosinase to inhibit the activity of the tyrosinase, so that the formation of melanin is reduced; the vitamin C inhibits the formation of melanin by reducing the formation of o-quinone and reducing oxidized melanin; the reduced glutathione is used as an antioxidant component, can inhibit the generation of free radicals, helps to stabilize the skin and restore the skin whiteness, can be used for treating chloasma, and provides a new solution for the field of skin health treatment.

The invention discloses a hepatocyte cryopreservation solution which comprises a first cryopreservation mixed solution, a second cryopreservation mixed solution and fetal calf serum, the first cryopreservation mixed solution is prepared from a Leibovitz's L-15 culture medium, lactobionic acid, raffinose, reduced glutathione, ademetionine, curcumin, bicyclol, ursodesoxycholic acid, silymarin and glycyrrhizic acid; the second cryopreservation mixed solution comprises cane sugar, trehalose, hydroxyethyl starch and polyene phosphatidylcholine. On the other hand, the invention discloses a cryopreservation method and a recovery method of primary mouse hepatocytes based on the hepatocyte cryopreservation liquid. The cryopreservation method comprises the following steps: pre-culturing, re-suspending, slowly dropwise adding DMSO (dimethylsulfoxide) and cooling and cryopreserving step by step. The resuscitation method comprises the steps of rapid unfreezing, centrifugal purification and resuscitation culture. The liver cell cryopreservation liquid and the cryopreservation and resuscitation method can play roles in regulating osmotic pressure, inhibiting ice crystal formation, stabilizing cell membrane structure, resisting oxidation, promoting protein synthesis and the like, and the resuscitated cells are high in survival rate and strong in adherence capability and functional activity.

The invention discloses a composition for protecting liver functions and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparations. The preparation method comprises the following steps: firstly, treating chrysanthemum in steps, preparing part of chrysanthemum into sterilized fine powder for later use, and adding part of chrysanthemum into an extraction system; the preparation method comprises the following steps: crushing and breaking shells of part of cassia seed and cistanche, mixing with Chinese angelica, performing ethanol percolation extraction, performing secondary water reflux extraction on decoction dregs and other traditional Chinese medicine raw materials, combining percolate and reflux filtrate, and concentrating; synchronously preparing a silybum marianum extract, a Chinese magnoliavine fruit extract and reduced glutathione which meet standards, spray-drying a concentrated solution, adding the chrysanthemum fine powder and the three types of core extracts, and performing three-dimensional mixing to obtain a finished product. The silymarin is used for enhancing liver cell membrane protection, the schisandra chinensis extract is used for activating an endogenous antioxidant system, the reduced glutathione is used for removing toxic substances, the traditional Chinese medicine components are used for synergistic interaction, meanwhile, the process is optimized to guarantee uniform component retention and distribution, and the problems that a traditional preparation is single in effect and unstable in quality are solved.

The application discloses a creatine kinase determination kit and a use method thereof. The creatine kinase determination kit comprises an R1 component and an R2 component. The R1 component comprises oxidized glutathione and auxiliary material 1. The R2 component comprises reduced coenzyme II, glucose-6-phosphate dehydrogenase, creatine phosphate, glutathione reductase and auxiliary material 2. The oxidized glutathione (GSSG) is reduced into reduced glutathione (GSH) by catalytic degradation of glutathione reductase (GR), and the reduced glutathione contains -SH, thereby activating CK. By the above method, the -SH compound N-acetyl-L-cysteine does not need to be added, thereby fundamentally solving the problem that -SH is gradually oxidized and deactivated, greatly improving the long-term stability of the reagent, and prolonging the effective period of the reagent.

The invention discloses a multi-component synergistic selenium-containing antibacterial system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. According to the method, water is adopted as a preparation medium, sodium selenite and a surfactant are added into the water, and reduced glutathione is used for reduction, so that nano-selenium colloid is prepared; subsequently adding an ammonium salt regulator and an alcohol regulator, and stirring to obtain the selenium-containing antibacterial system. The obtained selenium-containing antibacterial system has good broad-spectrum antibacterial activity.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention belongs to the technical field of gene editing, and particularly relates to an efficient grouper gene editing method based on fertilized egg injection. The method comprises the following steps: soaking fertilized eggs of groupers fertilized within 10 minutes in a weakening chorion culture medium buffer solution at 26 DEG C for 30-60 minutes, then taking out the fertilized eggs, and injecting a target CRISPR / Cas9 mixture into the fertilized eggs of the groupers before embryos develop to a four-cell stage; the attenuated chorion culture medium buffer solution is prepared by using sterilized seawater and comprises 102.8 mM of sodium chloride, 10mM of trihydroxymethyl aminomethane, 20mM of glycine and 1mM of reduced glutathione. According to the application, the rapid hardening of the chorion of the fertilized egg of the grouper can be effectively weakened, after the hardening time is prolonged to a four-cell stage, a relatively long operation window stage is obtained for micro-injection, the injection efficiency and the injection success rate can be improved, and the injection problem of the fertilized egg of the grouper is solved.

The invention discloses a method suitable for large-scale production of recombinant conopeptide. The conopeptide fusion protein containing the HIS tag-TrxA tag protein-enterokinase recognition site-conopeptide is constructed, a redox pair (reduced glutathione / oxidized glutathione) is introduced in vitro in the separation process, a redox reaction system is optimized, the mismatch rate of the recombinant conopeptide can be remarkably reduced, and the yield of the recombinant conopeptide is increased by more than one time. In addition, the enzyme digestion system is further optimized, and the recombinant conopeptide with high purity and correct structure is finally obtained. The invention provides an effective solution for large-scale production of conopeptide, and has huge application value.

The application discloses gold nanoclusters selectively combined with G-quadruplexes, a preparation method and application thereof, and the preparation method comprises the following steps: synthesizing gold nanoclusters by means of a hydrothermal reaction of reduced glutathione, N, N, N-trimethyl-(11-mercapto undecyl) ammonium chloride and chloroauric acid, wherein the preparation method is simple, the prepared gold nanoclusters are water-soluble gold nanoclusters capable of being specifically combined with G-quadruplexes, can easily enter into cell nuclei to be combined with G-quadruplex DNA, and exhibit an anti-tumor effect.

The present invention relates to the field of biotechnology, and specifically relates to PxGSTD2 or a mutant thereof, and the use thereof. More specifically, provided is a PxGSTD2 protein mutant comprising a mutation (F205) of a phenylalanine (F) residue at position 205 on the amino acid sequence as shown in SEQ ID NO. 3. Further provided is the use of PxGSTD2 or a mutant thereof in the aspects such as catalyzing the conjugation of a pesticide or Fusarium toxin, such as vomitoxin, with reduced glutathione (GSH), providing a target organism with tolerance to a Fusarium toxin such as vomitoxin, providing a food crop with tolerance or resistance to Fusarium-related diseases or other diseases, improving the yield or quality of a food crop, and improving the tolerance of a non-target organism to pesticides.

The invention belongs to the technical field of antioxidant drugs, and particularly relates to a glutathione dry powder inhalant as well as a preparation method and application thereof. The glutathione dry powder inhalant is prepared from the following active ingredients in percentage by mass: 50%-60% of reduced glutathione, 10%-20% of a carrier and 20%-30% of a dispersing agent, totaling 100%, the carrier is mannitol, lactose or trehalose, the dispersing agent is leucine, and no other pharmaceutic adjuvants exist. The glutathione dry powder inhalant is obtained by uniformly mixing the raw materials of the glutathione dry powder inhalant and then carrying out spray drying.

The invention discloses bacillus subtilis carbon nano enzyme as well as a preparation method and application thereof. The bacillus subtilis carbon nano-enzyme is prepared by adopting a solvothermal method, and the enzyme is obtained by carrying out high-temperature reaction, dialysis, rotary evaporation, centrifugal concentration, repeated freeze thawing, centrifugation, filtration and the like on bacillus subtilis, reduced glutathione and formamide, and finally carrying out vacuum freeze drying. The bacillus subtilis carbon nano-enzyme prepared by adopting a green and simple method can realize efficient and green degradation of residual pesticides in the environment, can improve the photosynthetic efficiency of plants and promote growth, and has a wide application prospect.

The invention provides an enzyme-labeled antibody protective agent and application of the enzyme-labeled antibody protective agent in an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit. The provided enzyme-labeled antibody protective agent is prepared from 0.1 to 0.5 percent of aminated mesoporous silicon dioxide nano particles, 0.5 to 2 percent of bovine serum albumin, 5 to 9 percent of complex saccharides, 0.01 to 0.1 percent of poloxamer 188, 0.01 to 0.05 percent of reduced glutathione and 0.02 to 0.05 percent of Proclin-300. The protective agent provided by the invention can effectively maintain the structure and activity of the enzyme-labeled antibody under various adverse conditions such as temperature fluctuation, oxidative stress and repeated freezing and thawing. The protective agent has broad-spectrum stability, not only can significantly reduce the activity loss of the antibody in the storage, transportation and use processes, but also can maintain the native conformation of the enzyme and the antibody by slowing down the oxidation effect, thereby avoiding inactivation and aggregation, and significantly prolonging the storage life and the use cycle of the antibody.

The application discloses a kind of attenuated salmonella and amodiaquine combined medicine and application, attenuated salmonella and amodiaquine combined medicine include attenuated salmonella typhimurium VNP20009 and its derivative or genetically modified strain and amodiaquine as active component.Amodiaquine is selectively eliminated neutrophil in tumor microenvironment by targeting binding glutathione reductase in neutrophil and inhibiting its activity.Amodiaquine of the application directly targets glutathione reductase in neutrophil, inhibits its activity, reduces reduced glutathione level, increases active oxygen accumulation, selectively induces neutrophil apoptosis in tumor immune microenvironment, and further enhances the colonization of VNP20009 and its derivative or genetically modified strain in tumor.The combination therapy reduces tumor weight by about 50%, prolongs tumor growth doubling time by 1.5 times, and does not affect the neutrophil level in peripheral blood and important organs, significantly reduces the liver and kidney toxicity of VNP20009 and its derivative or genetically modified strain.

The invention discloses radix puerariae and hovenia dulcis thunb health-care vinegar with a liver protection function and a preparation method and application thereof, the radix puerariae and hovenia dulcis thunb health-care vinegar is prepared from a traditional Chinese medicine composition and Shanxi mature vinegar, the traditional Chinese medicine composition is prepared from radix puerariae, hovenia dulcis thunb and schisandra chinensis according to the mass ratio of 2: 1: 1, and the mass-volume ratio of the traditional Chinese medicine composition to the Shanxi mature vinegar is 1: (0.5-5); a carbon tetrachloride-induced mouse chemical liver injury model and an ethanol-induced rat alcoholic liver injury model verify that the radix puerariae-fructus trifolii-schisandra chinensis vinegar can remarkably reduce the activity of alanine transaminase (ALT) and aspartate transaminase (AST) in serum, improve the content of reduced glutathione (GSH) in the liver, reduce the content of malondialdehyde (MDA) and triglyceride (TG) in the liver, and improve the activity of the radix puerariae-fructus trifolii-schisandra chinensis vinegar. The traditional Chinese medicine composition has a definite auxiliary protection effect on chemical liver injury and alcoholic liver injury. The invention provides a hangover-alleviating and liver-protecting product which is clear in component, synergistic in effect and supported by scientific data.

The invention discloses a preserving fluid for preserving pancreatic cancer tissues and application of the preserving fluid. The preserving fluid is prepared from mannitol, reduced glutathione, allopurinol, magnesium sulfate, gabexate mesylate, orlistat, pyruvic acid, Primocin, dexamethasone, heparin, bovine serum albumin, HEPES and the balance of an advance DMEM / F12 culture medium. The preserving fluid has a good effect in maintaining the pancreatic cancer tissue activity and inhibiting self-digestion and oxidative damage, is beneficial to maintaining the cell activity, and is beneficial to the preservation of pancreatic ductal adenocarcinoma in-vitro tissues and the construction of subsequent organoid.

The application belongs to the technical field of medical materials, and particularly relates to an alkaline phosphatase enzyme label buffer and an alkaline phosphatase enzyme label reagent. The alkaline phosphatase enzyme label buffer in the technical scheme of the application comprises a first chelate formed by nitrilotriacetic acid (NTA), diethylene triamine pentaacetic acid (DTPA) and magnesium ions and a second chelate formed by methionine, threonine and zinc ions, and further comprises the following components in percentage by mass: 0.2-2.0% reduced glutathione, 1.0-2.5% corn peptide, 0.5-10.0% protein, 0.1-2.0% ginsenoside, 0.1-1.0% surfactant, 1.0-5.0% saccharide compound, 1.0-10.0% polyhydric alcohol, 0.05-0.1% preservative and 0.01-0.05% antibiotic, and the rest is non-ionic amphiphilic buffer. The first chelate formed by nitrilotriacetic acid, diethylene triamine pentaacetic acid and magnesium ions and the second chelate formed by methionine, threonine and zinc ions are used in combination, and glutathione, protein and other substances are combined to obtain the alkaline phosphatase enzyme label buffer with long-term stability.