118 results about "Reduce glutathione" patented technology

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The invention discloses a leukemia targeted therapy preparation as well as a preparation method and application thereof. Comprising a DNA nanoflower carrier targeting leukemia cells through a PTK7 receptor and benzene arsenic oxide loaded on the carrier. The DNA nanoflower carrier is obtained by rolling circle amplification, and a nucleic acid aptamer complementary sequence of targeted binding leukemia cells is introduced into a template chain subjected to rolling circle amplification. The preparation method comprises the following steps: modifying sulfydryl onto a DNA nanoflower by using DNA-Dithiol, then breaking a disulfide bond by using reduced glutathione to expose the sulfydryl, and loading benzene arsenic oxide onto the DNA nanoflower through a reaction between trivalent arsenic and the sulfydryl. The structure of the DNA nanoflower has a high surface area and high porosity, and can load a large amount of PAO, so that the killing performance on cancer cells is enhanced; the aptamer sgc8 sequence is introduced into the DNA nanoflower, so that the targeting of the DNA nanoflower to cancer cells is effectively realized; the preparation also has the advantages of being good in thermodynamic stability, easy to store, excellent in biocompatibility and the like, and has a very good application prospect.

The invention belongs to the technical field of health-care food, and particularly relates to a composition and oral drops with the effects of dispelling the effects of alcohol, protecting the liver and relieving hangover as well as a preparation method and application of the composition and the oral drops. The invention provides a composition taking silybin eutectic crystal, curcumin eutectic crystal, ampelopsis grossedentata extract, pyrroloquinoline quinone disodium salt, reduced glutathione, alpha-ketoglutaric acid, vitamin, hericium erinaceus extract, frankincense extract, licorice extract and the like as main active ingredients, and the composition is subjected to scientific formula screening and functional verification, and has the advantages that the composition has the obvious curative effect, and the curative effect is obvious; the traditional Chinese medicine composition can effectively improve the metabolism of ethanol, protect liver and gastric mucosa, and is helpful for relieving hangover-related symptoms such as fatigue, thirst, sleepiness, headache, dry mouth, stomachache, nausea, hypomnesis, inattention and the like.

The invention discloses a water-based synthesis method of mu-conotoxin KIIIA with an instant wrinkle removal effect, and belongs to the technical field of biological medicines. A solid-phase synthesis method is adopted, H-RINK amide CHEMMATRIX resin is used as a solid-phase synthesis carrier, amino acid residues protected by substituent groups are sequentially condensed in an acetonitrile aqueous solution according to the sequence of amino acids from the C end to the N end of a main chain of mu-conotoxin KIIIA, and reduced crude polypeptide is obtained; and carrying out oxidative cyclization on the reduced crude polypeptide in an aqueous solution containing trihydroxymethyl aminomethane hydrochloride, guanidine hydrochloride, reduced glutathione and oxidized glutathione to obtain the mu-conotoxin KIIIA. The method is simple in process, environment-friendly, low in cost, low in chemical waste yield and high in yield (22-27%).

The invention relates to a purification and renaturation method of a recombinant chimeric protein inclusion body, which comprises the following steps: adding arginine into an inclusion body renaturation solution to improve the stability of renatured protein, adding reduced glutathione and oxidized glutathione at the same time, and adjusting the concentration, proportion and other process parameters of the reduced glutathione, the oxidized glutathione and the reduced glutathione to obtain the recombinant chimeric protein inclusion body. The renaturation effect and the yield of the denatured inclusion body protein are further improved.

The invention discloses iron oxide nanoparticles capable of responding to particle size change in a tumor microenvironment and a preparation method of the iron oxide nanoparticles, and belongs to the technical field of biomedical nano materials. The nano-particle comprises an iron oxide nano-crystal core with the particle size of 3-5nm and a tumor microenvironment response layer coating the surface of the iron oxide nano-crystal core. The response layer contains groups, such as disulfide bonds, enzyme digestion peptide fragments and the like, which can respond to acidic pH, high-concentration reduced glutathione or matrix metalloproteinase and other tumor characteristic stimuli to generate structural changes. In a tumor microenvironment, the hydration particle size of the nanoparticles can be directionally and controllably converted from 3-20 nm to 20-100 nm (or reversely). The preparation method mainly comprises two steps of preparing the core by a thermal decomposition method and modifying the surface response layer. The technical problems of insufficient tumor targeted enrichment capacity, limited imaging contrast and difficulty in balancing permeation and retention caused by fixed particle size of the existing iron oxide nanoparticles are solved, and the accuracy and efficiency of tumor diagnosis and treatment can be remarkably improved.

The invention belongs to the technical field of plant biology, and particularly relates to a tissue culture medium and a tissue culture method for culturing ginseng stem cells. The invention provides a tissue culture medium and a tissue culture method for culturing ginseng stem cells. The tissue culture medium comprises a germination medium, a growth medium, an induction medium and a proliferation medium. Under the combined action of NAA, 2, 4-D, 6-BA, KT, gibberellin GA3, acid hydrolyzed casein, carbon nanotubes, activated carbon, reduced glutathione and vitamin C, the tissue culture medium provided by the invention is beneficial to induction and proliferation of calluses and further conversion of the calluses into stem cells, so that browning of the calluses is effectively prevented. Results of the embodiment show that the tissue culture medium can induce ginseng seeds to germinate in a short time, the browning rate is 10%, the browning rate is low, a large number of ginseng stem cells can be rapidly obtained, and a foundation is laid for application of the ginseng stem cells.

The invention discloses a tumor treatment molecule with a curative effect self-amplification function. Belongs to the technical field of biotechnology and new medicine, and comprises a peptide sequence Ac-Asp-Glu-Val-Asp-Cys (StBu)-Lys combined to a CBT part and a paclitaxel chemotherapy molecule connected to a lysine side chain. After the probe enters a tumor cell, the probe is specifically recognized by reducing glutathione highly expressed in the tumor cell, and an Ac-DEVDCK (PTX)-CBT structure is generated; then, a weak cell apoptosis phenomenon is initiated through a molecular grafted paclitaxel structure; the Ac-DEVD fragment is used as a high-specificity polypeptide substrate of cysteine-aspartic acid specific protease 3 which is necessary in a cell apoptosis pathway, and can be specifically cut by the cysteine-aspartic acid specific protease 3, so that the Ac-DEVDCK (PTX)-CBT is converted into CK (PTX)-CBT; then, CK (PTX)-CBT molecules are subjected to a CBT-Cys click reaction to form a cyclic dimer, and the cyclic dimer is self-assembled to form particles. According to the method, the affinity of the paclitaxel to the tubulin in the cytoskeleton is utilized, so that the tubulin in the cell is aggregated, the self-amplification effect of the curative effect of the cell is realized, and the cell is triggered to generate a pan-apoptosis effect.

The present invention provides a magnetic metal-polyphenol network material based on β-cyclodextrin and a preparation method and application thereof, belonging to the technical field of magnetic metal-polyphenol network material preparation method. The method obtains dopamine-derived cyclodextrin by substitution reaction; prepares magnetic nanoparticles by solvothermal method; 4+ The coordination between them forms a metal-polyphenol network on the surface of magnetic nanoparticles, i.e., the host; the reduced glutathione is modified on the adamantane to form the guest; finally, the host and the guest are mixed to obtain a magnetic metal-polyphenol network material based on β-CD; the present invention utilizes hydrophilic action to enrich glycosylated peptides, and utilizes Ti 4+ Phosphorylated peptides are enriched by coordination with phosphate ions; the paramagnetism of the internal Fe3O4 magnetic nanoparticles allows the enriched glycosylated peptides and phosphorylated peptides to be easily and quickly separated from biological samples with the assistance of external magnets, thereby improving the efficiency of the entire enrichment process.

The invention provides a high-entropy nano enzyme. The chemical formula of the high-entropy nano enzyme is Mn < 0.4 > Co < 0.4 > Ni < 0.4 > Cu < 0.4 > Zn < 0.4 > Fe < 2.0 >) O. The invention also provides a preparation method of the high-entropy nano-enzyme, which comprises the following steps: dissolving manganese nitrate tetrahydrate, cobalt nitrate hexahydrate, nickel nitrate hexahydrate, copper nitrate trihydrate, zinc nitrate hexahydrate and sodium citrate in deionized water to prepare a clear solution A; dissolving potassium ferricyanide in deionized water to prepare a clear solution B; directly pouring the solution B into the solution A, stirring, standing and aging the obtained mixed solution at room temperature, and centrifugally collecting a product; and calcining the sample powder in a muffle furnace to obtain the high-entropy nano-enzyme. The invention further provides a catalyst which is composed of the high-entropy nano enzyme, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2. The invention also provides a method for detecting reduced glutathione by using the microfluidic paper chip analysis device. The invention provides a new normal form for constructing a multifunctional biosensing and environment monitoring platform.

The invention discloses ultralow-temperature preservation liquid for Sinilabeo decorus tungting sperms as well as a preparation method, a preservation method and application of the ultralow-temperature preservation liquid and belongs to the technical field of artificial breeding of Sinilabeo decorus tungting. The ultralow-temperature preservation liquid comprises a freezing diluent, an antifreeze agent glycol and an antioxidant, the antifreeze agent accounts for 8% of the volume of the ultralow-temperature preservation liquid, the antioxidant comprises reduced glutathione and alpha-lipoic acid, and the balance is the freezing diluent. According to the present invention, the formula compounding is different from the simple superposition of the traditional formula, the ratio and the combination mode of the components are precisely optimized, the combination has the synergistic effect, the resuscitation sperm activity is improved, the good oxidation resistance is provided, and the effect far better than the effect of other schemes is achieved. The method has remarkable advantages in efficient preservation of the sinilabeo decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus decorus

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

The present invention relates to the field of antioxidant peptides, and in particular to cottonseed antioxidant peptide E5R and its application. The present invention separates a cottonseed antioxidant peptide with high antioxidant activity from cottonseed protein, and determines its sequence to be ETEDACR. The antioxidant effect of the cottonseed antioxidant peptide is stronger than that of reduced glutathione at the same concentration; the cottonseed antioxidant peptide of the present invention can alleviate cellular oxidative stress. As a natural antioxidant, it is used in food, medicine, cosmetics and feed additives.

The invention belongs to the technical field of leavening agents, and specifically relates to a sourdough leavening agent and its preparation and application. The sourdough leavening agent, in parts by weight, comprises: 50-80 parts of lactic acid bacteria powder, 1-5 parts of yeast powder, 100-1000 parts of bran, 300-500 parts of yeast extract, 0.1-0.3 parts of plant extract, 0.1-0.2 parts of reduced glutathione, 500-1000 parts of flour and 500-1000 parts of water. The sourdough leavening agent is not only controllable in fermentation, but also does not require activation and expansion culture when used, is easy to use, convenient for transportation and storage, and is suitable for large-scale industrial production; and it utilizes lactic acid bacteria + yeast for synergistic fermentation, has strong acid production ability and fast fermentation speed, while retaining the flavor of traditional fermented foods, reduces the contamination of miscellaneous bacteria, and improves food safety; the sourdough leavening agent can also be applied to Chinese pastries, Western pastries, and traditional rice and flour fermented products to increase flavor, improve taste, improve texture, and extend the shelf life of products.

A method of measuring oxidative stress in a cell or tissue sample, the method comprising the steps of: (i) treating the cell or tissue sample in a culture medium; (ii) collecting a portion of the culture medium; (iii) measuring the amount of reduced glutathione (GSH) and oxidized glutathione (GSSG) in the cell or tissue sample; and (iv) measuring the amount of reactive oxygen species (ROS) in the culture medium portion collected in step (ii); wherein step (iii) is performed immediately after steps (i) and (ii); and wherein step (iv) is performed concurrently with step (iii), or within 6 hours after step (iii) is completed if the collected medium portion is stored at about 4 DEG C prior to performing step (iv), including the determination time.

The present invention discloses a formulation for alleviating plasticizer stress in crops and its application, belonging to the technical field of safe agricultural product production. This invention, for the first time, reveals the beneficial effects of reduced glutathione in alleviating plasticizer stress on crops. The use of reduced glutathione can significantly reduce the accumulation of plasticizers in crops, alleviating the inhibitory effects of plasticizers on crop growth. Furthermore, it proposes the application of reduced glutathione in crop production to effectively control and reduce plasticizer contamination of crops and avoid the adverse effects of plasticizers on the quality and safety of agricultural products.

The invention relates to hair-blacking essence and a preparation method thereof. Screening a symbiotic bacterium metabolism extract with small molecule peptide metabolism regulation capability; by combining low-concentration azelaic acid and zinc lactate components, scalp grease oxidation and abnormal proliferation of propionibacterium are inhibited; the expression of IL-10 anti-inflammatory factors is induced, the expression of TNF-alpha and IL-6 is reduced, and the hair follicle microinflammation state is reduced; a Wnt / beta-catenin pathway activator is used for inducing the melanin stem cells to be in a static state; the FGF-2 and the EGF are combined in a micro-dose manner to promote the amplification into precursor cells; mITF regulation peptides and a small amount of trace copper ions are supplemented to induce directional differentiation into mature melanocytes; a microcapsule carrier system with a three-layer structure; the penetration efficiency is improved; antioxidative peptide rich in reduced glutathione, NAD and a promoting factor are used, so that melanocytes are protected from oxidative damage; a coenzyme Q10 type mitochondrial homeostasis regulator is introduced, so that the energy metabolism is improved, the cell aging is delayed, the targeting property is strong, and the effect is accurate.

The invention relates to the technical field of feed additives, in particular to a Chinese herbal medicine ultrafine powder feed additive and a preparation method thereof, the following raw materials are respectively weighed: codonopsis pilosula, acanthopanax, hawthorn, andrographis paniculata, citric acid, catechin, L-ascorbyl palmitate and reduced glutathione; immersing the codonopsis pilosula sections, the acanthopanax sections and the haw slices into enzymatic hydrolysate to carry out enzymatic hydrolysis reaction; crushing the codonopsis pilosula, the acanthopanax, the hawthorn and the andrographis paniculata sections subjected to enzymolysis by using a hammer crusher to obtain mixed powder; adding citric acid, catechin, L-ascorbyl palmitate and reduced glutathione, and mixing; the mixture is subjected to superfine grinding through a jet mill, and superfine powder is obtained; and mixing the superfine powder with hydroxypropyl-beta-cyclodextrin, adding water to prepare slurry, stirring and clathrating, and then carrying out spray drying to obtain the Chinese herbal medicine superfine powder feed additive.

The present disclosure describes a human umbilical cord mesenchymal stem cell culture medium and a preparation method thereof, wherein the human umbilical cord mesenchymal stem cell culture medium includes a basal culture medium, reduced glutathione, epidermal growth factor, basic fibroblast growth factor, recombinant human insulin, hyaluronic acid and fibronectin, and the human umbilical cord mesenchymal stem cell culture medium does not include animal serum, and the reduced glutathione concentration is 1 mg / L to 3 mg / L, the epidermal growth factor concentration is 30 μg / L to 80 μg / L, the basic fibroblast growth factor concentration is 10 μg / L to 30 μg / L, the recombinant human insulin concentration is 1 mg / L to 5 mg / L, the hyaluronic acid mass fraction is 0.1% to 0.6%, and the fibronectin concentration is 10 mg / L to 50 mg / L. According to the present disclosure, a human umbilical cord mesenchymal stem cell culture medium and a preparation method thereof that does not contain animal serum, can support primary cell culture, is conducive to cell proliferation in vitro, adheres to the wall and maintains stemness can be provided.

The present invention belongs to the field of biomedicine and materials, and specifically relates to a protein-coupled imidazoquinoline compound and a synthesis method and application thereof. The present invention aims at the problem that imidazoquinoline agonists have large toxic side effects and are prone to cause cytokine storms when used in vivo. By coupling the active amino group of the imidazoquinoline agonist with albumin, which has low human immunogenicity, good biosafety and accumulation advantage at tumor sites, through a reduced glutathione-responsive linker, the targeting and responsive release at the tumor site are achieved, thereby reducing systemic nonspecific toxicity, reducing the possibility of causing cytokine storms, improving the immunosuppressive microenvironment of the tumor, activating anti-tumor immunity, and achieving better therapeutic effects and biosafety.

The invention provides application of hydroxypropyl chitosan oligosaccharide in preparation of a plant growth regulator, and belongs to the technical field of plant growth. The hydroxypropyl chitosan oligosaccharide is used as a raw material to prepare the plant growth regulator, new application of the hydroxypropyl chitosan oligosaccharide is provided, the seedling height of plants can be effectively increased, the root length can be effectively increased, the number and / or weight of root nodules can be effectively increased, the number of the root nodules of the plants and / or the activity of nitrogenase can be effectively increased, and the yield of the plants is increased. According to the present invention, the activity of glutathione reductase (GR) and / or glutathione transferase (GST) is improved so as to increase the reduced glutathione content, enhance the oxidation resistance of plants and / or enhance the salt resistance of crops, and after the compound fertilizer is compounded with the calcium-magnesium element fertilizer, the use amount of the compound fertilizer is reduced so as to provide effects of application reducing and effect increasing. The method for preparing the plant growth regulator is simple and easy to implement, environmentally friendly and convenient to popularize and apply.

The present invention provides an improved method for culturing mesenchymal stem cells and a culture medium used therefor, belonging to the technical field of cell culture. The improved mesenchymal stem cell culture medium provided by the present invention comprises the following exogenous additive components: recombinant human serum albumin, recombinant transferrin, recombinant human epidermal growth factor, basic fibroblast growth factor, platelet-derived growth factor, insulin, L-glutamine, reduced glutathione, β-mercaptoethanol, sodium selenite, penicillin-streptomycin, allicin, heparin and a proliferation promoter; the proliferation promoter comprises paeoniflorin, total saponins of Panax notoginseng, wolfberry polysaccharide and alcohol extract of Saussurea involucrata. By using the above culture medium for culturing mesenchymal stem cells, the cell activity and proliferation ability of mesenchymal stem cells can be significantly increased, the cell viability is increased by more than 50%, and the cell proliferation ability is increased by more than 40%, having great application potential in the culture of mesenchymal stem cells.

The invention provides a cascade response self-assembly polypeptide for remodeling tumor cell antigen composition, a bioactive solution of the cascade response self-assembly polypeptide and application of the cascade response self-assembly polypeptide. The polypeptide sequentially comprises a hydrophobic end-capping group, an alkaline phosphatase response self-assembly polypeptide sequence, a reduced glutathione response sequence and a T cell epitope peptide sequence. The polypeptide can respond to high-expression alkaline phosphatase in a tumor microenvironment to generate self-assembly and promote efficient internalization of cells; then, the antigen peptide is released under the action of reductive glutathione in tumor cells, and the antigen complex is given to the tumor cells through a main histocompatibility complex I-type molecular antigen presentation pathway. In addition, the specific hydrophobic end-capping group can up-regulate expression of I-type molecules of main histocompatibility complexes of tumor cells, enhance antigen presentation and remarkably enhance the recognition and killing efficiency of antigen-specific T cells on the tumor cells. Combined adoptive immunity and immune checkpoint inhibitor therapy is suitable for combined immunotherapy of solid tumors.

The application discloses a molecular probe and a preparation method and application thereof, and relates to the technical field of fluorescent probes.The structural formula of the molecular probe is as follows: the application takes two identical corrole units covalently connected as a parent body, and simultaneously introduces a reduced glutathione response group into the two corrole units, so that a molecular probe, namely a double-corrole molecular probe, which can remove reduced glutathione and realize NIR-II fluorescent signal opening, is constructed.The molecular probe can induce cell ferroptosis by consuming reduced glutathione in tumor cells.In addition, the molecular probe itself has a high photothermal conversion efficiency, and can perform photothermal treatment on tumor cells.Therefore, the molecular probe provided by the application is a companion diagnostic reagent integrating NIR-II fluorescent response, ferroptosis induction and photothermal treatment, has excellent antitumor effect, and has a wide application prospect.

The present invention provides nitrogen-, silicon-, and sulfur-codoped graphene quantum dots (GQDs) prepared by hydrothermal synthesis from waste carbon powder and reduced glutathione, wherein the mass ratio of waste carbon powder to reduced glutathione is 2:(4.5-5.5). The prepared GQDs exhibit high quantum yield, excellent stability, good water solubility, and good fluorescence properties. A N,Si,S-GQDs / OPD ratiometric fluorescent probe for autocatalytic rapid visual detection of Ag(I) and Fe(III) has been established. OPD is rapidly oxidized by Ag(I) / Fe(Ⅲ) to generate 2,3-diaminophenazine (oxOPD). Due to the inner filter effect between oxOPD and N,Si,S-GQDs, the fluorescence of N,Si,S-GQDs is significantly quenched. With the increase of Ag(I) / Fe(Ⅲ) concentration, the fluorescence of N,Si,S-GQDs at 440nm gradually weakens, while the fluorescence of oxOPD at 560nm gradually increases. Under ultraviolet light, the color of the solution gradually changes from blue to orange-yellow, enabling semi-quantitative visual detection.

The invention relates to oral probiotic hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing inulin-modified thiolated polysaccharide with a probiotic liquid, and quickly crosslinking to form probiotic hydrogel, so as to construct a colon-targeted probiotic delivery system; the system can effectively protect the probiotics from passing through the upper digestive tract, and realizes targeted release of the probiotics at the colon part by using a dual mechanism that the inulin is specifically ingested by the probiotics in the colon and the disulfide bond is easily reduced and disassembled by reduced glutathione (GSH) in the colon. The oral probiotic hydrogel disclosed by the invention is applied to preparation of medicines for preventing and treating irritable bowel syndrome, diarrhea, constipation, dyspepsia, inflammatory bowel disease, colon cancer, diabetes mellitus, obesity and hypercholesteremia.

The application discloses a kind of photonic nitric oxide donor with fluorescent self-reporting function and its preparation method and application, belong to the field of biomedical materials.The nitric oxide donor has the structure as shown in formula 1.The donor itself weak fluorescence, but in the presence of reduced glutathione (GSH), after irradiation by ultraviolet light (such as 405 nm), it can efficiently and controllably release nitric oxide (NO), and it is accompanied by strong fluorescence enhancement signal (fluorescence intensity can be increased by about 100 times), so as to realize the real-time, high-resolution fluorescence monitoring of NO release process and site.The donor has good light stability and biocompatibility.Experiments show that it can realize the light-controlled release and imaging of NO in living cells, and has application potential in the field of biomedical research and related drug development.

A perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na+; a source of 10 to 20 mM K+; a source of 5 to 10 mM Mg2+; a source of 0.25 to 0.75 mM Ca2+; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (IviES), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or N / -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO3; 1 to 30 mM glucose; 1 to 20 U / L insulin; 1 to 10 mM fructose diphosphate or a salt thereof, 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and, optionally, a diluent. The present disclosure also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

The invention provides application of an environmental response type anti-oxidation system to regulation and control of mitochondrial homeostasis and heart regeneration. The present invention relates to a reduced glutathione-modified polydopamine melanin nanoparticle, and particularly provides a reduced glutathione-modified polydopamine melanin nanoparticle, a method for promoting transformation of M1 macrophages to M2 macrophages by using the reduced glutathione-modified polydopamine melanin nanoparticle, a method for promoting damage repair of target cells, and a pharmaceutical composition.

The invention belongs to the technical field of antioxidant drugs, and particularly relates to application of a glutathione dry powder inhaler in preparation of drugs for treating radiation acute lung injury, the glutathione dry powder inhaler comprises the following active ingredients by mass percentage: 50%-60% of reduced glutathione, 10%-20% of a carrier and 20%-30% of a dispersant, totaling 100%, the carrier is mannitol, and the dispersing agent is L-leucine. According to the invention, the glutathione dry powder inhalant accurately delivers high-concentration GSH to an alveolar injury area through a lung targeting delivery system, synchronously activates endogenous antioxidant enzymes, inhibits lipid peroxidation and an inflammation source, and blocks release of proinflammatory factors, so that radiation-induced oxidation-inflammation cascade reaction is directly inhibited, alveolar structure damage is relieved, and the anti-inflammatory effect is achieved. And an efficient new treatment strategy is provided for RILI.