Process for producing reduced glutathione

一种氧化型谷胱甘肽、谷胱甘肽的技术，应用在肽的制备方法、化学仪器和方法、肽等方向，能够解决谷胱甘肽晶体阻塞电解槽等问题

Active Publication Date: 2013-12-18
KYOWA HAKKO BIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when oxidized glutathione is electrolytically reduced in the supersaturated region, glutathione rapidly crystallizes and precipitates due to voltage application, stirring, spray drying shock, etc., and the precipitated glutathione crystals may block the electrolysis groove

Method used

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  • Process for producing reduced glutathione
  • Process for producing reduced glutathione

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Electrolytic Reduction of Oxidized Glutathione (1)

[0051] Sodium hydroxide was added to the oxidized glutathione aqueous solution to adjust the pH to 6.5, thereby preparing a 400 g / L oxidized glutathione aqueous solution. As the electrolytic cell, an anode cell (15L) and a cathode cell (15L) are used, and the effective membrane area is 1.8dm 2 A cation exchange membrane SELEMION CMT (manufactured by Asahi Glass Co., Ltd.) separates the two tanks. A platinized titanium plate was used as the anode, and a zinc plate was used as the cathode. The distance between the electrodes and the cation exchange membrane was set to 1.9 mm, and the circulation flow rate was set to 240 L / hour. The anode tank contained a 0.50 mol / L sulfuric acid solution (5 L), and the cathode tank contained the aqueous oxidized glutathione solution (400 g / L, 5 L) prepared above.

[0052] At electrolysis voltage 6V, electrolysis current 6.6-8.3A / dm 2 And the electrolytic reduction reaction is carrie...

Embodiment 2

[0055] Electrolytic Reduction of Oxidized Glutathione (2)

[0056] Sodium hydroxide was added to adjust the pH of the solution to 4.5, thereby preparing a 400 g / L oxidized glutathione aqueous solution. Electrolytic reduction was performed under the same conditions as in Example 1, whereby it was confirmed that 1.73 kg of reduced glutathione was produced within 52 hours (conversion rate 86.7%).

[0057] The amount of zinc ions in the cathode tank solution after completion of electrolysis was confirmed in the same manner, thereby confirming the dissolution of 430 mg of zinc.

Embodiment 3

[0059] Electrolytic Reduction of Oxidized Glutathione (3)

[0060] Use graphite electrode as cathode and use the oxidized glutathione solution under the same conditions as in Example 1, at electrolysis voltage 6V, electrolysis current 6.6-8.3A / dm 2 electrolytic reduction reaction at room temperature. The resulting product in the cathode cell was quantified by HPLC under the same conditions as in Example 1, and it was confirmed that 1.82 kg of reduced glutathione was produced within 70 hours (conversion rate 90.7%).

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Abstract

A process for producing reduced glutathione by performing the electrolytic reduction of oxidized glutathione using a cathode vessel and an anode vessel which are separated from each other by means of a separating membrane, wherein an aqueous oxidized glutathione solution of which the pH value is adjusted to a value higher than 3.0 and not higher than 7.0 by adding a base is used as an aqueous solution for the cathode vessel. In this manner, reduced glutathione can be produced using an aqueous solution containing oxidized glutathione that can also act as a conducting agent.

Description

technical field [0001] The present invention relates to a method for producing reduced glutathione by electrolytically reducing oxidized glutathione. Background technique [0002] As a method of electrolytically reducing a disulfide compound, a method using an alloy composed of two or more specific metals as a cathode is known (Patent Document 1). In addition, as a method of producing L-cysteine ​​by electrolytic reduction of L-cystine which is a kind of disulfide compound, it is known to use a cation exchange membrane as a diaphragm and use A method of acidifying an L-cystine solution with hydrochloric acid or the like (Patent Document 2). As a method of producing reduced glutathione by electrolytic reduction of oxidized glutathione, a method using an aqueous solution of oxidized glutathione acidified by adding an inorganic acid such as concentrated hydrochloric acid to the electrolytic cell on the cathode side is also known. method (Patent Document 3). [0003] However,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/08C07K1/02C25B3/04C25B3/25
CPCC07K5/0215C25B3/25
Inventor 福元健太城野光隆
Owner KYOWA HAKKO BIO CO LTD
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