Acipenser dabryanus sperm preserving fluid liquid and preparing method and application

A cryopreservation solution, the technology of sturgeon, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of changing the activity of antioxidant enzymes, reducing the viability of sperm, changing the osmotic pressure of sperm, etc., to prevent The effect of protein denaturation, reduction of DNA molecular damage, and sperm protection
CN104054697AInactive Publication Date: 2014-09-24YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Publication Date
2014-09-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses acipenser dabryanus sperm preserving fluid liquid and a preparing method and application. The acipenser dabryanus sperm preserving fluid liquid comprises saccharose, mycose, trihydroxy aminomethane, potassium chloride, reduced glutathione and carbinol. The acipenser dabryanus sperm preserving fluid liquid has simple components and is low in cryopreservation cost. The added reduced glutathione plays a good role in antioxygenation, well protects sperm plasma membranes and reduces damage to DNA molecules. The added mycose has the effects of preventing protein denaturation and effectively protecting sperms. By means of the acipenser dabryanus sperm preserving fluid liquid, motility of unfreezed sperms is higher than 70%, and the fertility rate reaches 45%.
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Description

technical field

[0001] The invention belongs to the field of low-temperature cryobiology, and in particular relates to a Dabry's sturgeon semen cryopreservation solution, a method for preparing the Dabry's sturgeon semen cryopreservation solution, and an application of the Dabry's sturgeon semen cryopreservation solution. This semen cryopreservation solution is suitable for Acipenser dabryi. Background technique

[0002] The ultra-low temperature cryopreservation technology of fish semen has important application value in fish genetic breeding research, fish breeding and germplasm resource protection. The research on ultra-low temperature cryopreservation technology of fish semen began in the 1950s. Scholars at home and abroad have done a lot of work on the semen cryopreservation technology of freshwater and seawater economic fish and endangered fish. The research on the cryopreservation technology of sturgeon semen started late, and at first it was mainly the cryopreservat...

Claims

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