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A kind of Dabry's sturgeon sperm cryopreservation solution and its preparation method and application

A cryopreservation solution, the technology of Dabry's sturgeon, which is applied in the preparation of Dabry's sturgeon semen cryopreservation solution, the application field of Dabry's sturgeon semen cryopreservation solution, can solve the problem of sperm osmotic pressure change, sperm viability reduction, change of antioxidant Enzyme activity and other issues, to achieve the effect of protecting sperm, reducing DNA molecular damage, and preventing protein denaturation

Inactive Publication Date: 2015-10-28
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have proved that sperm will produce oxygen free radicals or change the activity of antioxidant enzymes during cryopreservation, thereby causing membrane lipid peroxidation, resulting in changes in membrane lipid structure and sperm osmotic pressure, or directly attacking sperm DNA bases etc., eventually leading to a reduction in sperm viability

Method used

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  • A kind of Dabry's sturgeon sperm cryopreservation solution and its preparation method and application
  • A kind of Dabry's sturgeon sperm cryopreservation solution and its preparation method and application
  • A kind of Dabry's sturgeon sperm cryopreservation solution and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-9

[0055] Sucrose, Trehalose, Tris, Potassium Chloride, Reduced Glutathione, Absolute Ethanol, and Deionized Water. The raw materials of the above reagents were purchased from Sigma Company.

[0056] Preparation of storage solution:

[0057] 1) Sucrose stock solution with a concentration of 1M: the molecular weight of sucrose is 342, and the weight of sucrose required to prepare a solution with a volume of 100ml is 34.2g. Put the weighed sucrose into a beaker, first add 60ml of distilled water, and stir it with a glass rod to fully dissolve it , and then dilute the solution to 100ml.

[0058] 2) Trehalose stock solution with a concentration of 1M: the molecular weight of trehalose is 342, and the weight of trehalose required to prepare a solution with a volume of 10ml is 3.42g. Put the weighed trehalose into a 10ml centrifuge tube, first add 6ml of distilled water, and spin Tighten the cap of the centrifuge tube, shake the centrifuge tube up and down to fully dissolve the treha...

Embodiment 10

[0068] A kind of Dabry's sturgeon sperm cryopreservation liquid is used in the application of Dabry's sturgeon sperm cryopreservation, and its application process is as follows:

[0069] The formula and preparation method of the Dabry's sturgeon sperm cryopreservation solution are as shown in the above example 5, and the cryopreservation solution is prepared and used immediately.

[0070] Taking ultra-low temperature (-196°C) cryopreservation of 100ml Dabry's sturgeon semen as an example, the method of using Dabry's sturgeon sperm cryopreservation solution is illustrated.

[0071] 1), semen collection and testing

[0072] During the breeding season of Dabry's sturgeon (mid-March to early April), B-ultrasound examination of the gonad development status of the male broodstock was carried out, and artificial induction of labor was performed on the sexually mature broodstock. Squeeze the abdomen, collect clean semen without feces and sundries in a plastic bag, seal it after oxyge...

Embodiment 11

[0082] Utilize example 2-4, the Dabry's sturgeon sperm cryopreservation liquid prepared by the formula described in example 6-8, carry out the preservation and thawing and fertilization of seminal fluid according to the method described in embodiment 10, its result is as follows:

[0083]

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Abstract

The invention discloses acipenser dabryanus sperm preserving fluid liquid and a preparing method and application. The acipenser dabryanus sperm preserving fluid liquid comprises saccharose, mycose, trihydroxy aminomethane, potassium chloride, reduced glutathione and carbinol. The acipenser dabryanus sperm preserving fluid liquid has simple components and is low in cryopreservation cost. The added reduced glutathione plays a good role in antioxygenation, well protects sperm plasma membranes and reduces damage to DNA molecules. The added mycose has the effects of preventing protein denaturation and effectively protecting sperms. By means of the acipenser dabryanus sperm preserving fluid liquid, motility of unfreezed sperms is higher than 70%, and the fertility rate reaches 45%.

Description

technical field [0001] The invention belongs to the field of low-temperature cryobiology, and in particular relates to a Dabry's sturgeon semen cryopreservation solution, a method for preparing the Dabry's sturgeon semen cryopreservation solution, and an application of the Dabry's sturgeon semen cryopreservation solution. This semen cryopreservation solution is suitable for Acipenser dabryi. Background technique [0002] The ultra-low temperature cryopreservation technology of fish semen has important application value in fish genetic breeding research, fish breeding and germplasm resource protection. The research on ultra-low temperature cryopreservation technology of fish semen began in the 1950s. Scholars at home and abroad have done a lot of work on the semen cryopreservation technology of freshwater and seawater economic fish and endangered fish. The research on the cryopreservation technology of sturgeon semen started late, and at first it was mainly the cryopreservat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 厉萍危起伟席萌丹柳凌郭威乔新美
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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