Low temperature conservation method for Chinese mushroom strain
A technology of cryopreservation and strains, which is applied in the preservation of microbial resources and in the field of microorganisms, and can solve the problems that straw mushroom strains cannot be preserved in low temperature
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Embodiment 1
[0096] 1. Cultivation of straw mushroom strains:
[0097] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.
[0098] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)
[0099] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, cultivate it for 10 days, and set it aside.
[0100] 2. Preparation of trehalose solution: prepare a 30% trehalose solution, weigh 30 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.
[01...
Embodiment 2
[0103] 1. Cultivation of straw mushroom strains:
[0104] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.
[0105] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)
[0106] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, cultivate it for 8 days, and set it aside.
[0107]2. Preparation of trehalose solution: prepare a 40% trehalose solution, weigh 40 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.
[0108...
Embodiment 3
[0110] 1. Cultivation of straw mushroom strains:
[0111] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.
[0112] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)
[0113] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, culture for 14 days and set aside.
[0114] 2. Preparation of trehalose solution: prepare a 15% trehalose solution, weigh 15 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.
[0115] 3. Lo...
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