Low temperature conservation method for Chinese mushroom strain

A technology of cryopreservation and strains, which is applied in the preservation of microbial resources and in the field of microorganisms, and can solve the problems that straw mushroom strains cannot be preserved in low temperature

Active Publication Date: 2008-02-27
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for long-term cryopreservation of straw mushroom strains to solve the problem that straw mushroom strains cannot be stored at low temperature. and colleges and universities

Method used

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  • Low temperature conservation method for Chinese mushroom strain
  • Low temperature conservation method for Chinese mushroom strain
  • Low temperature conservation method for Chinese mushroom strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] 1. Cultivation of straw mushroom strains:

[0097] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.

[0098] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)

[0099] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, cultivate it for 10 days, and set it aside.

[0100] 2. Preparation of trehalose solution: prepare a 30% trehalose solution, weigh 30 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.

[01...

Embodiment 2

[0103] 1. Cultivation of straw mushroom strains:

[0104] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.

[0105] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)

[0106] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, cultivate it for 8 days, and set it aside.

[0107]2. Preparation of trehalose solution: prepare a 40% trehalose solution, weigh 40 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.

[0108...

Embodiment 3

[0110] 1. Cultivation of straw mushroom strains:

[0111] 1) The preparation of comprehensive potato culture medium: 20% Potato extract (potato juice) 1L, Glucose (glucose) 20g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g, Vitanib B1 (thiamine) Trace (trace amount), Agar (agar) 15g, pH6, aliquoted into 10mL / tube, sterilized at 15 lbs for 30 minutes and placed on an inclined plane for later use.

[0112] (Note: Take 200 grams of peeled potatoes, cut into small pieces, add 1000 ml of water and boil for 30 minutes, filter out the potato pieces, and make up the filtrate to 1000 ml, which is 20% potato juice.)

[0113] 2) Inoculate the Strain mushroom strain on fresh comprehensive potato slant medium, culture for 14 days and set aside.

[0114] 2. Preparation of trehalose solution: prepare a 15% trehalose solution, weigh 15 grams of trehalose, add 100 mL of pure water, dissolve and filter to sterilize. Trehalose was purchased from Guangzhou Huabai Food Additive Co., Ltd.

[0115] 3. Lo...

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Abstract

The invention discloses a long-time low-temperature preserving method of dried straw mushroom bacterial, which is characterized by the following: adding 10-40 percent mycose solution in the test tube of seeded dried straw mushroom bacterial to cover the culture medium in the slope of test tube; putting the tube slope in the cooling store at 4-6 deg.c; simplifying the operation; reducing cost; fitting for dried straw mushroom bacterial manufacturing enterprises, scientific unit and colleges.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to the technical field of preservation of microbial resources. Background technique [0002] Straw mushroom (Volvariella volvacea (Bull ex Fr) Sing) belongs to Basidiomycetes Agaricaceae Ophthalmaceae. Fresh straw mushrooms are tender and tender, and dried straw mushrooms are rich in fragrance and rich in nutrition, and also contain substances that inhibit the growth of cancer cells. Commonly eaten straw mushrooms are unopened fruiting bodies. The artificial cultivation of straw mushrooms began in China's Guangdong, Hunan and other provinces. In 1981, the world produced nearly 60,000 tons of fresh straw mushrooms, of which China (including Taiwan Province) produced more than 40,000 tons, ranking first in the world. Other producing countries include Thailand, Indonesia, the Philippines, and India. Straw mushroom is the most cultivated edible fungus in Guangdong. Ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 朱红惠郭俊王永红李燕旋孙晓棠陈美标羊宋贞
Owner GUANGDONG INST OF MICROORGANISM
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