Industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities

A technology of corn oligopeptides, sobering up and protecting the liver, which is applied in the field of deep processing of plant protein, can solve the problems of high F value corn oligopeptide industrial production, complex process, high bitterness value, etc., to achieve improved purity and activity, simple production process, good stability effect

Inactive Publication Date: 2016-06-08
乳山市华隆生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing technology for preparing high F value corn oligopeptides has problems such as high cost, complex process, degree of automation, low product yield, purity and hepatoprotective activity, and high bitterness value.
As a result, the current high F value corn oligopeptides are limited to exploratory experimental research in the laboratory, and the industrial production of high F value corn oligopeptides has not yet been realized.

Method used

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  • Industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities
  • Industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities

Examples

Experimental program
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Effect test

Embodiment example 1

[0023] (1) Emulsification of CGM: Add water to 100kg of CGM according to the ratio of material to liquid ratio of 1:9, and emulsify in an emulsification tank at 8000r / min high-speed shear for 30min.

[0024] (2) Enzymolysis, enzyme inactivation, filtration: transfer the emulsion into an enzymolysis tank, adjust the temperature to 50°C, pH9, add alkaline protease and compound flavor protease according to the enzyme activity ratio of 3:1, and perform enzymolysis for 1-3 hours. After the enzymatic hydrolysis is completed, the temperature of the enzymatic hydrolysis tank is raised to 90°C to inactivate the enzyme for 10 minutes. After inactivating the enzyme, perform frame filtration.

[0025] (3) Activated carbon adsorption: adjust the pH of the above filtrate to 2.5, add activated carbon at a solid-to-liquid ratio of 1:10, and statically adsorb for 2 hours. And perform plate and frame filtration.

[0026] (4) Separation and purification: the above filtrate is subjected to grad...

Embodiment example 2

[0029] (1) Emulsification of CGM: Add water to 100kg of CGM according to the ratio of material to liquid ratio of 1:9, and emulsify in an emulsification tank at 9000r / min high-speed shear for 45min.

[0030] (2) Enzymolysis, enzyme inactivation, filtration: transfer the emulsion into an enzymolysis tank, adjust the temperature to 55°C, pH 10, add alkaline protease and compound flavor protease according to the enzyme activity ratio of 4:1, and enzymatically hydrolyze for 2 hours. After the enzymatic hydrolysis is completed, the temperature of the enzymatic hydrolysis tank was raised to 95°C to inactivate the enzyme for 13 minutes. After inactivating the enzyme, use a frame filter to filter the enzymatic hydrolyzate.

[0031] (3) Activated carbon adsorption: adjust the pH of the above filtrate to 3, add activated carbon at a solid-to-liquid ratio of 1:10, and statically adsorb for 2.5 hours. And perform plate and frame filtration.

[0032] (4) Separation and purification: the ...

Embodiment example 3

[0035] (1) Emulsification of CGM: Add water to 100kg of CGM according to the ratio of material to liquid ratio of 1:9, and emulsify in an emulsification tank at 10000r / min high-speed shear for 60min.

[0036] (2) Enzymolysis, enzyme inactivation, filtration: transfer the emulsion into an enzymolysis tank, adjust the temperature to 60°C, pH 11, add alkaline protease and compound flavor protease according to the enzyme activity ratio of 5:1, and enzymatically hydrolyze for 3 hours. After the enzymatic hydrolysis is completed, the temperature of the enzymatic hydrolysis tank is raised to 100°C to inactivate the enzyme for 15 minutes. After inactivating the enzyme, use a frame filter to filter the enzymatic hydrolyzate.

[0037] (3) Activated carbon adsorption: Adjust the pH of the above filtrate to 3.5, add activated carbon at a solid-to-liquid ratio of 1:10, and statically adsorb for 3 hours. And perform plate and frame filtration.

[0038] (4) Separation and purification: the...

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Abstract

The invention discloses an industrial production method of high-F-value corn oligopeptide with alcohol dispelling and liver protection activities. The production method comprises the following steps: carrying out high-speed shearing emulsification on corn gluten meal (CGM), carrying out enzymolysis by combining basic protein with compound flavored proteinase, carrying out enzyme deactivation and frame filtering, carrying out activated carbon adsorption to remove bitterness, colors and aromatic amino acid, carrying out separation and purification by virtue of ultrafiltration equipment, carrying out desalination by virtue of nanofiltration, and carrying out spray drying, so as to obtain the high-F-value corn oligopeptide. According to the high-F-value corn oligopeptide produced by virtue of the production method, the fatty degeneration index of liver cells of mice with alcoholic hepatic injury can be decreased, meanwhile, the contents of triglyceride (TG) and malondialdehyde (MDA) in the livers can be reduced, and the content of reduced glutathione (GSH) can be increased; and a result shows that the high-F-value corn oligopeptide produced by virtue of the production method has the effect of preventing the alcoholic hepatic injury of the mice.

Description

technical field [0001] The invention relates to an industrial production method of a high F-value corn oligopeptide with the activity of sobering up and protecting the liver, and belongs to the field of deep processing of vegetable protein. Background technique [0002] High F value corn oligopeptide is a mixed small peptide system composed of 2-6 amino acid residues with a molecular weight below 1000Da prepared from corn yellow powder (CGM), and its notable feature is the molar ratio of branched chain amino acids to aromatic amino acids (F value) is relatively high, generally greater than 20. High F value corn oligopeptides have various physiological functions such as anti-fatigue, supplementing essential amino acids for human body, promoting alcohol metabolism, protecting liver and liver, and improving protein nutrition status of postoperative and bedridden patients. my country is rich in CGM powder resources, with an annual output of more than 300,000 tons, which has not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/14A23J3/34
CPCA23J3/14A23J3/34A23V2002/00
Inventor 张文镨王溢张明振
Owner 乳山市华隆生物科技股份有限公司
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