Preserving fluid of hepatic cells for biological artificial liver and preparation method thereof

Abstract

The invention provides preserving fluid of hepatic cells for a biological artificial liver and a preparation method thereof. The preserving fluid is a solution compounded by ultrapure water. The solution contains the following components within the concentration range: 15-25mmol/L of disodium hydrogen phosphate, 1-10mmol/L of sodium hydrogen phosphate dehydrate, 4-6mmol/L of potassium citrate monohydrate, 10-30mmol/L of sodium chloride, 5-10mmol/L of magnesium chloride hexahydrate, 3-10mmol/L of disodium adenosine triphosphate, 1-5mmol/L of reducing glutathione, 0.1-0.5mmol/L of alpha-lipoic acid, 100-150mmol/L of trehalose (C6H12O5), 200/0.510-50g/L of hydroxyethyl starch and 2-10mg/L of matrine. The preparation method of the preserving fluid comprises the following steps of: accurately weighing all components according to the concentration requirements of the components, wherein the alpha-lipoic acid is weighed in a dark place; completely dissolving the other components except the alpha-lipoic acid by using the right amount of ultrapure water; sufficiently dissolving the alpha-lipoic acid in the dark place; and adding the ultrapure water to full dose. The preserving fluid can well protect the cell activity of the hepatic cells for the biological artificial liver and the special functions of the hepatic cells at low temperature so as to satisfy the short-term low temperature preservation of a large-scale hepatic cell bank for the biological artificial liver and/or the hepatic cell protection in the long-distance transportation process.

Description

Technical field [0001] The present invention relates to the field of in vitro preservation liquid biotechnology of human or animal organs, tissues or cells, and in particular to a preservation liquid of hepatocytes for bioartificial liver and a preparation method thereof. Background technique [0002] my country is an area with a high incidence of liver disease. Approximately 1.2 million cases of acute viral hepatitis occur every year, of which severe hepatitis accounts for about 0.2% to 0.4%, and the case fatality rate can be as high as 70%. About 1% of hepatitis B patients can develop acute or subacute liver failure. About 20% of patients with acute hepatitis B can become chronic, and about 3% of patients can develop post-hepatitis cirrhosis. About 60% to 80% of hepatitis C patients become chronic, 20% can develop cirrhosis, and 12% develop end-stage liver disease. Approximately 300,000 cases of liver disease die every year, of which 50% are primary hepatocellular carcinoma, ...

AI Technical Summary

Problems solved by technology

UW solution is currently the most widely used multi-organ perfusion preservation solution in the world. It can effectively preserve kidney for 72 hours, pancreas for 72 hours, liver for 30 hours, and heart for 24 hours. However, it still has many shortcomings. Its hypertonicity causes cell wrinkles Shrinkage, high viscosity affect in situ perfusion, high potassium is not in line with physiology, lack of drugs to protect liver cryopreservation, expensive

HTK solution, like UW solution, is an ideal organ preservation solution. The biggest advantage of HTK solution is its low viscosity, which can improve the microcirculation of preserved organs and increase tissue oxidation. It still has obvious shortcomings mainly reflected in In terms of the limitation of organ preservation time, the incidence of graft non-function will increase significantly as time goes on

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