The invention discloses a preparation method of an antistatic silica gel protective film. The preparation method comprises following steps: 1, a substrate layer is prepared; 2, methyl vinyl silicone rubber, polyisoprene rubber, silicone resin, vinyl silicone oil, hydrogen-containing silicone oil, 3-thiocyanopropyltriethoxysilane, 2-aminobenzimidazole, nanometer graphite powder, lauryl alcohol phosphoric acid ester potassium, toluene, and ethyl acetate are uniformly mixed and stirred, methylbutynol, platinum catalyst, and cyclohexanone peroxide are stirred uniformly to prepare an organic silicagel adhesive; 3, the surface of the substrate layer is uniformly coated with the organic silica gel adhesive, and curing is carried out so as to form an organic silica gel layer and obtain a semi-finished protective film; and 4, the semi-finished protective film is cut into strips based on need so as to obtain the antistatic silica gel protective film. The antistatic silica gel protective film islow in surface resistivity; and in using process of the antistatic silica gel protective film, no static discharging is caused, so that a problem in the prior art that automatic continuous product isinfluenced by static discharging is solved.