A carbendazim-degrading bacterium mbc-6f and its application

A carbendazim and bacterial technology, applied in the field of microbiology, can solve the problems of increasing the stillbirth rate, destroying the development of mammalian uterus, interfering with the development of adult mouse sperm and testis

Inactive Publication Date: 2017-09-19
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Reproductive toxicity: Carbendazim is an endocrine disruptor that can disrupt the development of mammalian uterus and interfere with the development of sperm and testis in adult mice
(3) Embryo toxicity: Male mice fed carbendazim for 60 days were mated with normal female mice, and it was found that although the

Method used

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  • A carbendazim-degrading bacterium mbc-6f and its application
  • A carbendazim-degrading bacterium mbc-6f and its application
  • A carbendazim-degrading bacterium mbc-6f and its application

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Embodiment Construction

[0025] Further illustrate below in conjunction with accompanying drawing and specific test example that the inventor provides

[0026] 1. Screening, isolation and purification of carbendazim-degrading bacteria:

[0027] (1) Test sample: Greenhouse soil that was sampled in May 2013 and continuously applied carbendazim to the College of Horticulture, Northwest A&F University.

[0028] (2) culture medium

[0029] LB liquid medium: NaCl 10.0g, peptone 10.0g, yeast extract powder 5.0g, add distilled water 1000mL, adjust the pH value to 7.0.

[0030] Basic salt medium: NaCl 1.0g, K2HPO4 1.5g, KH2PO4 0.5g, (NH4)2SO4 2.0g, MgSO4·7H2O 0.2g, distilled water 1000mL, pH=7.0.

[0031] LB solid medium: same as LB liquid medium, add 1% to 2% agar.

[0032] Enrichment (degradation) test medium: same as basic salt medium, add carbendazim as needed.

[0033] Separation and purification medium: the same as the enrichment (degradation) test medium, adding 1% to 2% agar.

[0034] All the abov...

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Abstract

The invention discloses a carbendazim-degrading bacterium MBC‑6F, which was preserved in the China Center for Type Culture Collection on December 31, 2014, with the preservation number CCTCC NO: M 2014680. The carbendazim-degrading bacteria MBC‑6F was isolated and screened in the soil. It was identified as Microbacterium sp.MBC‑6F by 16S rDNA, which can directly produce specific degradation effect on carbendazim. According to HPLC analysis, MBC‑6F can degrade carbendazim within 72 hours. Completely degrade 40µM carbendazim, the optimum degradation temperature is 37℃, and the optimum degradation acidity is pH 7.0. The main degradation products determined by HPLC-MS are 2-aminobenzimidazole and 2-hydroxybenzimidazole. The 16S rDNA sequence of the strain was disclosed for the first time, as well as the degradation efficiency and product analysis of carbendazim.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, relates to a new genus Microbacterium (Microbacterium), in particular to a bacterium of the genus Microbacterium MBC-6F capable of degrading carbendazim (carbendazim, MBC) and the degradation of carbendazim by the bacterium Determination. Background technique [0002] Carbendazim (carbendazim), also known as cotton wilting, befenti, benzimidazole No. 44, English trade name MBC, relative molecular mass is 191.2, molecular formula is: C 9 h 9 N 3 0 2 , Carbendazim is a broad-spectrum fungicide. Since 2011, the annual usage has been about 40,000 tons. In 2012, the number of its registered products reached 857. There are nearly ten kinds of emulsions and seed coatings. In my country, carbendazim is widely used in the prevention and control of diseases of wheat, fruit trees, tea leaves, vegetables, etc., and is also used for post-harvest preservation of fruits. In agriculture, it is mainl...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/205C12R2001/01
Inventor 呼世斌雷霁陈鹏
Owner NORTHWEST A & F UNIV
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