Fluorescent polymer based on aggregation-induced emission effect as well as preparation method and application thereof

A technology of aggregation-induced luminescence and fluorescent polymers, applied in the fields of botanical equipment and methods, applications, luminescent materials, etc., can solve the problems of poor particle uniformity, low content of fluorescent molecules, and limited wide application, and achieve the effect of surface cleanliness

Active Publication Date: 2020-09-04
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method can prepare uniform and stable nanoparticles, but the surface of the prepared nanoparticles contains a large amount of emulsifier, which limits the wide application of this method.
The co-precipitation method is currently the main way to prepare polymer nanoparticles. The specific implementation method is to dissolve the fluorescent molecules in a water-soluble organic solvent, and then add the resulting solution dropwise to a water-soluble polymer solution. Fluorescent nanoparticles can be obtained under the action of
[0003] On the other hand, most of the fluorescent agent molecules used in the preparation process of traditional polymer fluorescent nanoparticles have the phenomenon of aggregation-caused quenching (Aggregation-caused quenching). The existence of this phenomenon makes the fluorescent molecules in the prepared fluorescent nanoparticles The content is very low, and it is impossible to obtain a polymer fluorescent molecule with a higher fluorescent molecule loading rate

Method used

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  • Fluorescent polymer based on aggregation-induced emission effect as well as preparation method and application thereof
  • Fluorescent polymer based on aggregation-induced emission effect as well as preparation method and application thereof
  • Fluorescent polymer based on aggregation-induced emission effect as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of polymer particles containing AIE properties with controllable particle size: five sets of polymerization reaction solutions were respectively prepared, and the composition of each reaction solution was consistent. Among them, 0.05g of maleic anhydride, 0.04g of styrene, 0.005g of divinylbenzene, 0.01g of TPE-VBC, and 0.001g of azobisisobutyronitrile were added to a 50mL single-necked flask, and then 10mL of butyl acetate was added to The complete dissolution of the above reagents constitutes a set of polymerization reaction solutions. After the above five groups were passed through nitrogen and deoxygenated for 10 minutes, they were placed in a water bath at 50°C for polymerization. Take out a group of polymerization reaction solution at intervals of 10 minutes, centrifuge and wash three times with a mixed solution of ethyl acetate and petroleum ether, then place the product in a vacuum oven at 50°C, and vacuum-dry to obtain fluorescent polymer particles ...

Embodiment 2

[0056] Preparation of polymer fluorescent nanoparticles with a particle size of 500 nm: Take 0.05 g of maleic anhydride, 0.04 g of styrene, 0.005 g of divinylbenzene, 0.01 g of TPE-VBC, and 0.001 g of azobisisobutyronitrile, and add 50 mL of single port In the flask, 10 mL of butyl acetate was then added to completely dissolve the above reagents. After passing nitrogen to remove oxygen for 10 minutes, place it in a 60°C water bath for polymerization. Polymer particles with a particle diameter of about 500 nm were obtained after polymerization for 120 minutes.

[0057] protein coupling

[0058] 0.1 g of the obtained fluorescent polymer particles were re-dispersed into 10 mL of 0.1 M phosphate buffer solution (pH 7.4) by ultrasound, and then left for 3 hours to completely disperse the polymer particles in the buffer solution. Subsequently, 0.05 g of powdered lipase was added to the mixed solution, placed in a shaker at 37° C. for 60 minutes to react, and then the polymer parti...

Embodiment 3

[0060] Preparation of polymer fluorescent nanoparticles with a particle size of 200 nm: Take 0.05 g of maleic anhydride, 0.04 g of styrene, 0.005 g of divinylbenzene, 0.087 g of TPE-V, and 0.001 g of azobisisoheptanonitrile, and add 50 mL of single-port In the flask, 10 mL of amyl acetate was then added to completely dissolve the above reagents. After passing nitrogen to remove oxygen for 10 minutes, place it in a 60°C water bath for polymerization. After 20 minutes of polymerization, polymer particles with a particle diameter of about 200 nm were obtained.

[0061] Cell labeling experiments

[0062] Take 0.1 g of the resulting fluorescent polymer particles and re-disperse them into 10 mL of 0.1 M phosphate buffer solution (pH 7.4) by ultrasound, and then leave it for 3 hours to completely disperse the polymer particles into the buffer solution, and then use PBS to buffer The solution was diluted to obtain a 10 g / mL dispersion of polymeric particles. Place natural killer ce...

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Abstract

The invention discloses an aggregation-induced emission effect-based multifunctional fluorescent polymer which is controllable in particle size and convenient to modify as well as a preparation methodand an application of the aggregation-induced emission effect-based multifunctional fluorescent polymer. The fluorescent polymer contains a structural formula unit as shown in a formula (I) which isdescribed in the specification, n is equal to 10-10000, and R is a structural group with an aggregation-induced emission function. A precipitation polymerization reaction is carried out on an AIE polymerizable unit, maleic anhydride, styrene and an optional cross-linking agent in the presence of an initiator to obtain the fluorescent polymer. The fluorescent polymer can be applied to immunoregulation, cell labeling, material antibiosis and roughness detection.

Description

technical field [0001] The invention relates to the field of new materials, in particular to a multifunctional fluorescent polymer based on aggregation-induced luminescent effect with controllable particle size and easy modification, and its preparation method and application. Background technique [0002] In recent years, polymer fluorescent nanoparticles have shown more and more important roles and values ​​in the fields of biomedicine and optoelectronic devices. Especially in the field of biomedicine, such as cancer diagnosis and treatment and cell imaging, higher requirements are put forward for polymer fluorescent nanoparticles, such as particle size uniformity, surface modification and stability of particle structure, etc. At present, the methods for preparing polymer fluorescent nanoparticles mainly include the following four (Li K, LiuB. Polymer-encapsulated organic nanoparticles for fluorescence and photoacoustic imaging [J]. Chemical Society Reviews, 2014, 43(18): ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/08C08F212/08C08F212/36C08F212/00C09K11/06A01N43/08A01N43/10A01P1/00A01P3/00G01B11/30G01N21/64
CPCA01N43/08A01N43/10C08F222/08C09K11/06C09K2211/145C09K2211/1458G01B11/30G01N21/6428G01N21/6486C08F212/08C08F212/36C08F212/32
Inventor 顾星桂周良宇王冠杨万泰
Owner BEIJING UNIV OF CHEM TECH
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