3results about How to "Change function" patented technology

This invention discloses a strain of Aspergillus sp., DLF18, and its applications, belonging to the field of microbial technology, with accession number CGMCC NO.41737. This strain can effectively utilize rice straw and / or sugarcane bagasse to produce humic acid, with a humic acid content of 16.5% per gram of fermentation supernatant after rice straw fermentation. Applying the fermentation product of DLF18 to crops can significantly improve crop growth and increase the humic content in the soil. Simultaneously, metagenomic analysis results show that, compared with the control group, the application of DLF18 to the soil significantly altered species β-diversity, functional gene abundance, and inter-group differences. These findings indicate that DLF18 has broad application prospects in agricultural straw utilization and soil remediation.

The invention discloses an organophosphate hydrolase mutant and application of the organophosphate hydrolase mutant in degradation of triphenyl phosphate. The mutant is obtained by mutating valine at the 275th site of an amino acid sequence shown in SEQ ID NO.2 into aspartic acid V275D, leucine at the 139th site of the amino acid sequence shown in SEQ ID NO.2 into phenylalanine L139F and proline at the 256th site of the amino acid sequence shown in SEQ ID NO.2 into serine P256S. The invention also relates to a gene for coding the mutant, a recombinant vector, a recombinant genetically engineered bacterium and application thereof. Experiments show that the mutant has efficient degradation capacity on triphenyl phosphate, can degrade triphenyl phosphate in a water phase at normal temperature, and has high degradation efficiency and good application prospects.

The present application relates to a kind of deaminase mutant and its application, the deaminase mutant is obtained by the mutation of the amino acid sequence shown in SEQ ID NO.1, the mutation at least includes one in following mutation site: the 60th by L mutation A or V, the 100th by S mutation A or T, the 170th by G mutation S or C, the 200th by E mutation K or H.The deaminase mutant is applied to nucleoside compound, catalytic activity is high, stability is good, compared with wild type deaminase, there is different degree of improvement.The catalyzed reaction compared with chemical synthesis method is simple and mild, reaction selectivity is high, preparation cost is low, with good application prospect.