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37results about How to "Simple detection" patented technology

Method for monitoring the functioning of a NOx sensor arranged in an exhaust gas channel of an internal combustion engine

The invention relates to a method for monitoring the functioning of an NOx sensor, which is arranged in an exhaust gas channel of an internal combustion engine and is located downstream from an NOx storage catalytic converter. The aim of the invention is to detect the faulty functioning of the NOx sensor in a simple manner in order to be able to take appropriate measures if necessary. To this end, the mass of NOx absorbed by the NOx storage catalytic converter is determined within a diagnostic period using a measurement signal of the NOx sensor. At the same time, an absorbed NOx target mass is calculated using a model for the NOx storage catalytic converter. A ratio of the NOx mass to the NOx target mass is then compared to predetermined limit values to determine the functioning of the NOx sensor. The aim of the invention is alternatively accomplished by measuring a duration time for a complete NOx regeneration of the NOx storage catalytic converter. A specified duration time is also calculated for the NOx regeneration using a model for the NOx storage catalytic converter and using a measured or calculated NOx load state. A ratio of the measured duration time to the specified duration time is then compared to predetermined limit values to determine the functioning of the NOx sensor.
Owner:VOLKSWAGEN AG

PCR (polymerase chain reaction) primer for detecting tobacco soil-borne fungal pathogens as well as application and method of PCR primer

The invention discloses a PCR (polymerase chain reaction) primer for detecting tobacco soil-borne fungal pathogens as well as application and a method of the PCR primer. The PCR primer comprises a tobacco rhizoctonia solani primer, a phytophthora nicotianae primer, a thielaviopsis basicola primer and a pythium aphanidermatum primer; the application is application of the PCR primer to simultaneous detection of four tobacco soil-borne fungal pathogens, and four soil-borne fungi are tobacco rhizoctonia solani, phytophthora nicotianae, thielaviopsis basicola and pythium aphanidermatum; the method comprises the steps of pre-treating, PCR reaction and electrophoretic detection; the detection operation is simple, convenient, quick, high in sensitivity and accurate; a sample is prepared and detected within 4 hours; the four pathogens of tobacco rhizoctonia solani, phytophthora nicotianae, thielaviopsis basicola and pythium aphanidermatum are simultaneously detected; a DNA (deoxyribonucleic acid) sample of the pathogen of 0.01 ng can be detected; the accuracy of a detection result is higher than that in a conventional separation identification and symptom identification method.
Owner:YUNNAN ACAD OF TOBACCO AGRI SCI

Quality detection device for shipbuilding steel plate butt-jointed seams

The invention provides a quality detection device for shipbuilding steel plate butt-jointed seams, which comprises a detection tank, a detection tank hold-down mechanism, an alcohol jar, an alcohol detector, and an alarm. A seal rubber ring is arranged at the top of the detection tank connected with the alcohol jar through an air pipe. The detection tank hold-down mechanism comprises a support base board, a jack and clamping boards arranged at two ends of the support base board. The detection tank is arranged at the bottom of the shipbuilding steel plate butt-jointed seams, the support base board is arranged below the detection tank, the clamping boards at the two ends of the support base board are clamped on the shipbuilding steel plates, the jack is arranged between the support base board and the detection tank, the seal rubber ring is pressed on the bottom face of the shipbuilding steel plates which are located on two sides of one butt-jointed seam, the alcohol detector directly faces the top of the welding seam and is connected with the alarm through a signal line. By mean of the quality detection device for the shipbuilding steel plate butt-jointed seams, welding seam detection can be achieved before assembling and after butt welding of the shipbuilding steel plates, the detection is more simple and energy-saving, and the increased rework cost caused by welding seam defects after assembling can be effectively reduced.
Owner:CHONGQING SHENGZHU SCI&TECH DEVCO

Toxoplasma IgM antibody immunoblotting kit and preparation method thereof

The invention which relates to a kit concretely relates to a toxoplasma IgM antibody immunoblotting kit and a preparation method thereof. The kit is characterized in that: the kit is provided with a vector plate, a nitrocellulose film, a toxoplasma IgM antibody detection line and a contrast line; the toxoplasma IgM antibody detection line and the contrast line are sequentially arranged on the nitrocellulose film; the toxoplasma IgM antibody detection line is coated with a toxoplasma recombinant antigen, and the contrast line is coated with a human IgM antibody; and an anti-human mum-chain antibody is labeled with horseradish peroxidase. The preparation method comprises the following steps: 1, preparing the toxoplasma antigen; 2, carrying out nitrocellulose film spotting; 3, preparing an anti-human IgM specific fragment mum-chain monoclonal antibody; 4, labeling the anti-human IgM specific fragment mum-chain monoclonal antibody with the horseradish peroxidase; and 5, preparing the immunoblotting kit. The kit of the invention, which allows the needed sample amount to be extremely small and no special instrument to be needed during detection and allows the result to be directly interpreted by naked eyes, has the advantages of simple and rapid detection, strong specificity, high sensitivity, accuracy and reliability, low cost, and wide application.
Owner:厦门市仙岳医院

Hemicyanine compound based on benzothiazole biheterocycle, and preparation method and application thereof

The invention discloses a hemicyanine compound based on benzothiazole biheterocycle, and a preparation method and an application thereof. The structural formula of the compound is represented by formula I. The preparation method of the compound comprises the following steps: carrying out an alkylation reaction on a benzothiazole compound represented by formula II and alkyl halide RbX to obtain a substituted benzothiazole compound, and then carrying out a Knoevenagel condensation reaction on the substituted benzothiazole compound and N-ethylcarbazole-3-formaldehyde to obtain the target compound. The synthesis and preparation steps are simple and easy to implement, raw materials are cheap and easy to obtain, and the product serving as a probe is stable in structure and high in specificity and can be used for specifically detecting G-quadruplex and double-helix secondary structures of nucleic acid. A detection method of the probe for the nucleic acid secondary structure has the advantagesof low price, simplicity and convenience in operation, good selectivity and quickness, and overcomes the defects of high price, high equipment requirement and relatively complex technical operation of other detection methods.
Owner:INST OF CHEM CHINESE ACAD OF SCI

Non-invasion-type detection instrument and method for cerebral blood oxygen of fetus in abdomen

The invention discloses a non-invasion-type detection instrument and method for the cerebral blood oxygen of a fetus in the abdomen. The detection instrument comprises a probe and a control device, the probe completely fits the abdomen of a pregnant woman and is provided with a luminous source and multiple light sensing points, when light emitted by the luminous source penetrates through the front-wall tissue of the abdomen of the pregnant woman, the shallow-layer tissue of the head of the fetus and the cerebral tissue of the fetus, corresponding emergent light is formed, and the light sensingpoints detect the emergent light corresponding to the light sensing points; the control device is internally provided with a central processing module, a power module, an optical source driving module, a light sensing point signal receiving module and a data displaying module, and the power module, the optical source driving module, the light sensing point signal receiving module and the data displaying module are electrically connected with the central processing module; the optical source driving module controls the luminous source to give out light, the light sensing point signal receivingmodule receives light sensing signals of the light sensing points, and after the central processing module conducts data processing, a cerebral blood oxygen saturation degree value is obtained and displayed by the data displaying module. By adopting a noninvasive detection mode, the detection instrument can be applied to timely evaluation on the cerebral oxygen condition of the fetus in the abdomen, and the situation is avoided that due to events such as the cord around neck, the encephalic hypoxic-ischemic state is caused.
Owner:中科搏锐(北京)科技有限公司

Device for pressurizing and pressure maintaining anti-leak detection after corn harvester brake pump assembly

The invention discloses a device for pressurizing and pressure maintaining anti-leak detection after corn harvester brake pump assembly. The device comprises a base. A fuel tank bracket is fixedly arranged at one end on the base, and a pump fixing fixture is arranged at the other end. A hydraulic fuel recycling tank is arranged below the pump fixing fixture. A primary return fuel filter and a secondary return fuel filter are arranged at the bottom of the hydraulic fuel recycling tank. A multi-way valve group is arranged below the fuel tank bracket, and the multi-way valve group is connected with a main pump operating handle and a branch pump operating handle. A pressure gauge is mounted on a side of the fuel tank bracket. A hydraulic cylinder is arranged at the inner bottom of the fuel tank bracket. A telescopic rod at the front end of the hydraulic cylinder is provided with a bolt. A hydraulic fuel tank is fixedly mounted on the fuel tank bracket. The hydraulic fuel tank is provided with a fine return fuel filter and a hydraulic fuel tank cover. A power switch is mounted on one side of the base. The device is provided with the hydraulic cylinder, so that pressurizing detection of a main pump can be realized. Fuel leaked from a brake pump is filtered and recovered by the hydraulic fuel recycling tank so as to be recycled. The operating handles are connected with the multi-way valve group, which makes detection simple and quick.
Owner:YONGMENG MACHINERY CO LTD

Fluorescent quantitation PCR detection method of gyrovirus3 GyV3

The invention discloses a specific primer for detecting gyrovirus3 GyV3. The specific primer comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQID NO.2. A fluorescent quantitation PCR detection method of the gyrovirus3 GyV3 comprises the stepsof (1) performing a real-time fluorescent quantitation PCR reaction: extracting genome DNA of substances to be detected, performing PCR amplification through the specific primer, and deducing a standard linear regression equation (standard curve); and (2) performing result judgment: judging samples free from Ct value and free from an amplification curve to be feminine, and judging samples of whichthe Ct value is smaller than or equal to 36 and in which a typical amplification curve appears to be positive. According to the established method disclosed by the invention, safe, specific, quick, acute and simple quick detection of GyV3 can be realized, so that a method is provided for epidemiological investigation and early diagnosis of the gyrovirus3 GyV3, the specific primer has important significance, and the blank in relevant field at home and abroad is filled.
Owner:POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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