Test strip for rapid detection for transgene product

Abstract

The invention discloses a test strip for rapid detection for a transgene product. The test strip comprises a sample pad, a colloidal-gold combination pad, a nitrocellulose membrane detection zone andan absorbent pad; the colloidal-gold combination pad is coated with a colloidal gold-labeled digoxin antibody; the nitrocellulose membrane detection zone is provided with a detection line and a quality control line; the detection line is formed by coating streptavidin on the nitrocellulose membrane detection zone; the quality control line is formed by coating goat anti-mouse IgG on the nitrocellulose membrane detection zone; a reaction product of the product to-be-detected is dropped onto a sample pad, and then is chromatographed in the direction of the absorbent pad through capillary action.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to a test strip for rapidly detecting transgenic products. Background technique [0002] General transgenic detection is divided into nucleic acid level detection and protein level detection. [0003] Protein level detection (existing colloidal gold protein test paper technology) is convenient for detection, but because some transgenic sample proteins are not expressed and the protein is easily degraded, it is possible that the protein expression in the sample to be tested may not be expressed or the storage conditions of the sample to be tested may cause degradation. Therefore, false negative results are caused (positives are not detected). The protein test strip method has the advantages of rapidity, simplicity, and economy, and does not require any special equipment. However, due to its low detection sensitivity and narrow application range, it is main...

AI Technical Summary

Problems solved by technology

However, the PCR method requires high experimental facilities and conditions, and is time-consuming.

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