Fluorescent quantitation PCR detection method of gyrovirus3 GyV3
A circovirus, fluorescent quantitative technology, applied in the field of molecular biology, to achieve the effect of simple detection, accurate quantification, and rapid detection
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[0036] Example Fluorescence quantitative PCR detection of chicken new circovirus GyV3
[0037] 1. Related test pathogens: IBV, IBDV, ILTV, H5, H9, Reov, FAV, REV, ARV, CAV, all of which were identified and preserved by the Poultry Research Institute of Shandong Academy of Agricultural Sciences.
[0038] 2. Primer design: refer to the existing gene sequence of the chicken new circovirus GyV3, perform comparison, select the conservative region, and design specific primers for the PCR method for real-time fluorescent quantitative detection of chicken new circovirus GyV3. The present invention designs four pairs of primers (9-Gyv3, 10-Gyv3, 11-Gyv3, 12-Gyv3), and performs amplification and sensitivity comparison tests on these four pairs of primers, and finally screens out sequences with high sensitivity and good specificity Used in the establishment of this method.
[0039] The designed specific primer sequence is:
[0040] Upstream primer 9-GyV3 forward: 5'TCCAATAAGTTCGTCGGAGTC 3'(SEQ ...
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