A serum albumin detecting reagent and a serum albumin detecting method
A serum albumin and detection reagent technology, applied in the field of detection reagents, can solve the problems of low albumin results, lack of specificity, and low sensitivity, and achieve the effect of strong specificity, rapid detection, and guaranteed accuracy
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[0038] Example 1
[0039] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:
[0040] Reagent R1 includes: pH 5.0 buffer 20mmol / L, surfactant 0.01g / L, reaction accelerator 12.00g / L, electrolyte 50mmol / L, preservative 10.00g / L, stabilizer 0.10g / L.
[0041] Reagent R2 includes: pH 6.0 buffer 20 mmol / L, anti-human albumin antibody 2.00 g / L, electrolyte 50 mmol / L, preservative 10.00 g / L, and stabilizer 0.10 g / L.
[0042] Calibrators include: pH 6.0 buffer 20mmol / L, human albumin 3.00g / L, electrolyte 50mmol / L, preservative 10.00g / L, stabilizer 0.10g / L.
[0043] Among them, the buffer is Tris buffer, the surfactant is Tween20, the reaction accelerator is polyethylene glycol 2000, the electrolyte is sodium chloride, the preservative is sodium azide, the stabilizer is bovine serum albumin, anti-human albumin Antibody used goat anti-human albumin antibody.
[0044] The calibrators were diluted with buffer to obtain a series of calibrat...
Example Embodiment
[0050] Example 2
[0051] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:
[0052] Reagent R1 includes: pH 9.0 buffer 500 mmol / L, surfactant 2.00 g / L, reaction accelerator 0.10 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.
[0053] Reagent R2 includes: pH 6.5 buffer 300 mmol / L, anti-human albumin antibody 100.00 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.
[0054] Calibrators include: pH 6.5 buffer 300 mmol / L, human albumin 6.00 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.
[0055] Among them, the buffer is glycine buffer, the surfactant is Tween40, the reaction accelerator is polyethylene glycol 4000, the electrolyte is potassium chloride, the preservative is lithium azide, the stabilizer is gelatin, and the anti-human albumin antibody is rabbit Anti-Human Albumin Antibody.
[0056] The calibrator was diluted with buffer to obt...
Example Embodiment
[0062] Example 3
[0063] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:
[0064] Reagent R1 includes: pH 7.0 buffer 300 mmol / L, surfactant 1.00 g / L, reaction accelerator 6.00 g / L, electrolyte 250 mmol / L, preservative 5.00 g / L, and stabilizer 20.00 g / L.
[0065] Reagent R2 includes: pH 9.0 buffer 150 mmol / L, anti-human albumin antibody 50.00 g / L, electrolyte 300 mmol / L, preservative 5.00 g / L, and stabilizer 20.00 g / L.
[0066] Calibrators include: pH 9.0 buffer 150mmol / L, human albumin 5.00g / L, electrolyte 300mmol / L, preservative 5.00g / L, stabilizer 20.00g / L.
[0067] Among them, borate buffer is used as buffer, Span20 is used as surfactant, polyethylene glycol 6000 is used as reaction accelerator, magnesium chloride is used as electrolyte, gentamicin is used as preservative, HEDTA is used as stabilizer, and anti-human albumin antibody is used Goat anti-human albumin antibody.
[0068] The calibrators were diluted with buf...
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