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A serum albumin detecting reagent and a serum albumin detecting method

A serum albumin and detection reagent technology, applied in the field of detection reagents, can solve the problems of low albumin results, lack of specificity, and low sensitivity, and achieve the effect of strong specificity, rapid detection, and guaranteed accuracy

Inactive Publication Date: 2016-05-04
PUREBIO LAB NINGBO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the BCG method lacks specificity, and the results are high in patients with reduced albumin/globulin ratios such as hemodia

Method used

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  • A serum albumin detecting reagent and a serum albumin detecting method
  • A serum albumin detecting reagent and a serum albumin detecting method
  • A serum albumin detecting reagent and a serum albumin detecting method

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0038] Example 1

[0039] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:

[0040] Reagent R1 includes: pH 5.0 buffer 20mmol / L, surfactant 0.01g / L, reaction accelerator 12.00g / L, electrolyte 50mmol / L, preservative 10.00g / L, stabilizer 0.10g / L.

[0041] Reagent R2 includes: pH 6.0 buffer 20 mmol / L, anti-human albumin antibody 2.00 g / L, electrolyte 50 mmol / L, preservative 10.00 g / L, and stabilizer 0.10 g / L.

[0042] Calibrators include: pH 6.0 buffer 20mmol / L, human albumin 3.00g / L, electrolyte 50mmol / L, preservative 10.00g / L, stabilizer 0.10g / L.

[0043] Among them, the buffer is Tris buffer, the surfactant is Tween20, the reaction accelerator is polyethylene glycol 2000, the electrolyte is sodium chloride, the preservative is sodium azide, the stabilizer is bovine serum albumin, anti-human albumin Antibody used goat anti-human albumin antibody.

[0044] The calibrators were diluted with buffer to obtain a series of calibrat...

Example Embodiment

[0050] Example 2

[0051] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:

[0052] Reagent R1 includes: pH 9.0 buffer 500 mmol / L, surfactant 2.00 g / L, reaction accelerator 0.10 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.

[0053] Reagent R2 includes: pH 6.5 buffer 300 mmol / L, anti-human albumin antibody 100.00 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.

[0054] Calibrators include: pH 6.5 buffer 300 mmol / L, human albumin 6.00 g / L, electrolyte 500 mmol / L, preservative 0.10 g / L, and stabilizer 10.00 g / L.

[0055] Among them, the buffer is glycine buffer, the surfactant is Tween40, the reaction accelerator is polyethylene glycol 4000, the electrolyte is potassium chloride, the preservative is lithium azide, the stabilizer is gelatin, and the anti-human albumin antibody is rabbit Anti-Human Albumin Antibody.

[0056] The calibrator was diluted with buffer to obt...

Example Embodiment

[0062] Example 3

[0063] Serum albumin detection reagents, including reagent R1, reagent R2 and calibrators. Specifically:

[0064] Reagent R1 includes: pH 7.0 buffer 300 mmol / L, surfactant 1.00 g / L, reaction accelerator 6.00 g / L, electrolyte 250 mmol / L, preservative 5.00 g / L, and stabilizer 20.00 g / L.

[0065] Reagent R2 includes: pH 9.0 buffer 150 mmol / L, anti-human albumin antibody 50.00 g / L, electrolyte 300 mmol / L, preservative 5.00 g / L, and stabilizer 20.00 g / L.

[0066] Calibrators include: pH 9.0 buffer 150mmol / L, human albumin 5.00g / L, electrolyte 300mmol / L, preservative 5.00g / L, stabilizer 20.00g / L.

[0067] Among them, borate buffer is used as buffer, Span20 is used as surfactant, polyethylene glycol 6000 is used as reaction accelerator, magnesium chloride is used as electrolyte, gentamicin is used as preservative, HEDTA is used as stabilizer, and anti-human albumin antibody is used Goat anti-human albumin antibody.

[0068] The calibrators were diluted with buf...

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Abstract

The invention discloses a serum albumin detecting reagent. The serum albumin detecting reagent comprises a reagent R1, a reagent R2 and a calibration compound. The reagent R1 comprises a buffer liquid, a surfactant, a reaction accelerant, an electrolyte, an aseptic and a stabilizing agent. The reagent R2 comprises a buffer liquid, an anti-human albumin antibody, an electrolyte, an aseptic and a stabilizing agent. The calibration compound comprises a buffer liquid, serum albumin, an electrolyte, an aseptic and a stabilizing agent. The serum albumin detecting reagent is advantaged by high specificity and rapid and sensitive detection. The invention further discloses a serum albumin detecting method.

Description

technical field [0001] The invention relates to the technical field of detection reagents, in particular to a serum albumin detection reagent. The invention also discloses a serum albumin detection method. Background technique [0002] Serum albumin (Albumin, ALB) is the most abundant protein in plasma, accounting for 55% to 65% of total serum protein. It is synthesized by liver parenchymal cells and widely exists in blood, lymph, and muscle tissues of the human body. sex transport protein. Serum albumin is a routine liver function test item in clinical laboratories, and the determination of its content is of great significance to the diagnosis, treatment and prognosis of clinical diseases. The normal value of serum albumin in different age groups is also different, the normal range of newborn is 28-44g / L, the normal range of 14 years old is 38-54g / L, the normal range of adult is 35-50g / L, 60 The normal range after the age is 34-48g / L. Under normal circumstances, the inc...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/765
Inventor 林清菁范华王剑
Owner PUREBIO LAB NINGBO
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