Kit for identifying benign and malignant human thyroid nodules and use method and application thereof

A technology for thyroid nodules and kits, which is applied in biochemical equipment and methods, and the determination/examination of microorganisms, can solve the problems of high positive rate, low probability of negative missed diagnosis, unclear diagnosis, etc., and achieves simple detection and easy operation. , reduce the effect of misjudgment

Pending Publication Date: 2019-04-19
上海安甲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, in order to simultaneously detect somatic mutations of multiple thyroid cancer-specific pathogenic genes in thyroid nodule samples, it is necessary to break through the following scientific and technical difficulties: First, although a large number of thyroid Cancer-causing gene mutations, but amon

Method used

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  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof
  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof
  • Kit for identifying benign and malignant human thyroid nodules and use method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0096] 1.2. Example 2

[0097] The difference from Example 1 is that the PCR reaction system is shown in Table 7 below, and the components and contents of PCR buffer 1-PCR buffer 7 are shown in Table 8 below. The sample DNA of thyroid nodules is specifically analyzed by pathology. It was later judged to be a fresh pathological tissue of a benign thyroid nodule, with a DNA content of 40ng, and the subject was the same patient. The electropherogram is as follows image 3 As shown, the running tape of each gene fragment in the electrophoresis diagram is bright and clear, but a little blurry than that of Example 1. The electrophoresis diagram is basically the same as that of Example 1, and the measured gene sequence is also the same as that of Example 1.

[0098] Table 7 PCR reaction system

[0099]

[0100] Table 8: Components and contents of PCR buffer 1-PCR buffer 7

[0101]

Example Embodiment

[0102] 1.3. Example 3

[0103] The difference from Example 1 is that the PCR reaction system is shown in Table 9 below, and the components and contents of PCR buffer 1-PCR buffer 7 are shown in Table 10 below. The sample DNA of thyroid nodules is specifically analyzed by pathology. The postoperative frozen tissue, which was later judged to be benign thyroid nodules, had a DNA content of 100ng, and the subject was the same patient. The electropherogram is as follows Figure 4 As shown, the running tape of each gene fragment in the electrophoresis diagram is bright and clear, and the electrophoresis diagram is basically the same as that of Example 1, and the measured gene sequence is also the same as that of Example 1.

[0104] Table 9 PCR reaction system

[0105]

[0106] Table 10: Components and content of PCR buffer 1-PCR buffer 7

[0107]

[0108]

[0109] 1.4. Example 4

[0110] The difference from Example 1 is that the PCR reaction system is shown in Table 11 below, and the compone...

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Abstract

The invention belongs to the technical field of kits, and in particular relates to a kit for identifying benign and malignant human thyroid nodules and a use method and application thereof. The kit comprises a sequencing primer system, a PCR buffer system, Taq enzyme, UNG enzyme and a negative quality control substance. The sequencing primer system comprises a primer pair BRAF-2, a primer pair KRAS-2, a primer pair NRAS-2, a primer pair HRAS-3, a primer pair HRAS-4 and a primer pair TERT-2, which are used for measuring 19 mutation sites on a BRAF gene, a KRAS gene, a NRAS gene, a HRAS gene anda TERT gene. The kit can be used for simultaneously detecting a plurality of gene variations, the detection result can be taken as an intermediate result to provide assistance for clinically diagnosing benign and malignant thyroid nodules and judging prognosis, and the kit can be further combined with other results to verify whether the thyroid cancer is suffered or not, so that probability of misjudgment is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of kits, and in particular relates to a kit for distinguishing benign from malignant human thyroid nodules and its use and application. Background technique [0002] Thyroid nodules are a common thyroid disease in the population. Most nodules are benign and can be treated conservatively, but 5%-10% of thyroid nodules are malignant and require early surgical treatment. to obtain a good prognosis. If a large number of patients with benign thyroid nodules who do not need surgery are given surgical treatment, a huge medical insurance burden will be generated; on the contrary, if the real malignant tumor cannot be identified from a large number of benign thyroid nodules, the treatment of these patients will be delayed, endangering patient's life and health. Therefore, timely and accurate detection of benign and malignant thyroid nodules is very important. [0003] With the development of molecular biology tech...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686
CPCC12Q1/686C12Q1/6883C12Q2600/118C12Q2600/16C12Q2600/156C12Q2565/125C12Q2535/122C12Q2537/143
Inventor 刘磊
Owner 上海安甲生物科技有限公司
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