9 results about "Braf genes" patented technology

1. The name of the design product: kit (BRAF intestinal cancer gene detection typing kit). 2. The use of the design product: the design product is used for packaging reagents. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

The application belongs to the field of biological medicine, and relates to a composition, a kit and application for thyroid cancer detection. The primer composition can be used for simultaneously detecting mutation or fusion of five gene sites of two genes, BRAF gene and RET-PTC gene, and can be used for detecting thyroid cancer and has high sensitivity and specificity. Meanwhile, the fluorescence PCR technology is adopted to improve detection efficiency, shorten detection time and realize precise diagnosis of thyroid cancer.

The invention discloses a noninvasive exosome mRNA BRAF V600E mutation Tag-PCR (Tag-Polymerase Chain Reaction) detection method, which comprises the following steps: separating an exosome sample from a body fluid; the method comprises the following steps: by taking mRNA as a template, adding an exogenous reference gene and a primer pair for detecting the exogenous reference gene, and meanwhile, carrying out a first round of PCR amplification reaction by using a Tag-ARMS primer pair for specifically enriching exosome mRNA BRAF gene V600E mutation so as to form a PCR product; a PCR product is used as a template, a system without UNG enzyme is used for amplification, a primer pair for detecting a reference gene is added, a Tag primer pair for specifically enriching and detecting V600E mutation of the human exosome mRNA BRAF gene and a probe sequence are used at the same time, and then a second round of PCR amplification reaction is carried out; and judging the mutation state of the V600E site of the BRAF gene of the mRNA of the exosome sample.

The present invention relates to a compound for regulating the expression level and / or activity of the BRAF gene, and a composition comprising same. The compound and the composition are useful for preventing, treating, or alleviating BRAF-related diseases, disorders, and conditions.

Provided are methods for detecting urogenital malignancies in dogs. In some embodiments, the methods include identifying a deletion or single nucleotide substitution within a BRAF gene and / or within a MAP2K1 gene present in or isolated from a biological sample from a. dog, wherein the presence of the deletion or single nucleotide substitution within the BRAF gene and / or within the MAP2K1 gene detects a urogenital malignancy, optionally transitional cell carcinoma / urothelial carcinoma, in the dog. In some embodiments, the deletion is within exon 12 of BRAF gene, optionally within the amino acid sequence KMLNVTAPTPQQL (SEQ ID NO: 3), and / or in within exon 2 or 3 of a M.AP2K1 gene, optionally within the amino acid sequence FLTQKQKVGE (SEQ ID NO: 4).

The present application belongs to the technical field of intelligent medical treatment, and particularly relates to a RAS, BRAF gene wild type colorectal cancer liver metastasis patient curative effect prediction model based on image group characteristics and application thereof. The present application successfully constructs a model for realizing curative effect prediction of RAS, BRAF gene wild type colorectal cancer liver metastasis patients receiving bevacizumab combined with chemotherapy treatment in the late first line based on CT image group characteristics before treatment: the enhanced CT image group data before initial treatment of patients with advanced colorectal cancer liver metastasis at the time of initial diagnosis is collected, 7 image group characteristics are obtained by using 1000 times Lasso-Logistic analysis, and an image group prediction model is constructed by using a multi-factor logistic regression method. The model constructed by the present application has important clinical application and popularization value from the clinical actual problem.

This invention, entitled "A Biomarker Detection Reagent, Kit, and Application for Colorectal Cancer Gene Mutation Detection," belongs to the field of biomedical technology. The technical problem to be solved is to provide a biomarker detection reagent capable of comprehensively detecting colorectal cancer gene mutations while ensuring high sensitivity and high specificity, thus addressing the difficulty in simultaneously achieving comprehensive site coverage, high sensitivity, high specificity, and high accuracy in existing technologies. The key technical solution is a biomarker detection reagent for colorectal cancer gene mutation detection, comprising a combination of amplification primers for detecting mutation sites in KRAS, NRAS, PIK3CA, and BRAF genes. The amplification primer combination includes primers for amplifying blocked mutation systems and ordinary primers.

Provided is a methylation marker for gastric cancer detection. Provided are 28 differentially methylated regions (DMRs) for diagnosing or assisting with the diagnosis of cancer, and an ELMO1 gene and a KCNA3 gene with a better performance are selected from these 28 DMRs as preferred methylation markers for detecting gastric cancer. Provided is an accurate, simple and economical means for the early screening of gastric cancer, which can improve the detection rate of gastric cancer, particularly early gastric cancer, in gastric cancer high-risk populations and populations undergoing routine physical examinations, thereby improving the survival rate of gastric cancer patients, while saving on substantial medical expenses and alleviating the medical burden.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to a primer, a probe, a DNA template, a kit and a detection method for detecting human BRAF gene mutation. According to the invention, a real-time fluorescent PCR amplification reaction system is established to realize rapid detection of BRAF gene mutation; the operation is simple and the result is easy to read. The amplification of the internal standard substance can monitor the accuracy and reliability of the whole detection process by synchronously amplifying a known amount of internal standard substance, so that the stability of experimental data is ensured; the device plays an irreplaceable role in the aspects of guaranteeing the detection quality and improving the detection efficiency.