Fluorescent PCR detecting kit for V600E mutation of BRAF gene

A detection kit and mutation detection technology, which is applied in the field of medical detection, can solve the problems of poor tolerance of the kit, achieve fast operation, high detection accuracy, and prevent false negative effects

Inactive Publication Date: 2016-02-03
HUNAN SHENGWEI GENE TECH
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of BRAF gene V600E mutation fluorescent PCR detection kit, to solve the problem that the kit of existing BRAF gene mutation described in the technical background has poor tolerance

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  • Fluorescent PCR detecting kit for V600E mutation of BRAF gene
  • Fluorescent PCR detecting kit for V600E mutation of BRAF gene
  • Fluorescent PCR detecting kit for V600E mutation of BRAF gene

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Embodiment Construction

[0031] The BRAF gene V600E mutation fluorescent PCR detection kit provided in the embodiment of the present invention is to provide a BRAF gene mutation detection kit with rapid operation, simple method, high detection sensitivity and wide detection range.

[0032] In order to enable those skilled in the art to better understand the technical solutions in the embodiments of the present invention, and to make the above-mentioned purposes, features and advantages of the embodiments of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention are described below in conjunction with the accompanying drawings The program is described in further detail.

[0033] The BRAF gene V600E mutation fluorescent PCR detection kit provided in the embodiment of the present invention includes a V600E mutation detection reaction solution, and the V600E mutation detection reaction solution includes 5 μL of 10×PCR buffer, 0.2 mmol / L de...

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Abstract

The invention provides a fluorescent PCR detecting kit for V600E mutation of a BRAF gene. The detecting kit comprises a V600E mutation detection reaction solution, wherein the V600E mutation detection reaction solution comprises a 10*PCR buffering solution, deoxyribonucleoside triphosphate, an upstream primer, a downstream primer and a probe, wherein the upstream primmer and the downstream primer are used for amplifying target polynucleotide, and the probe is used for detecting the target polynucleotide. The fluorescent PCR detecting kit for V600E mutation of the BRAF gene is based on a real-time fluorescent quantitative PCR technology and an ARMS-PCR technology, has favorable specificity and is high in operation speed, simple and convenient in method and high in detection precision; and meanwhile an interior label additionally arranged in a reaction system can be used for effectively avoiding that the detected result is false negative.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a fluorescent PCR detection kit for BRAF gene V600E mutation. Background technique [0002] The BRAF gene is an important member of the EGFR (epidermal growth factor receptor, the receptor for epithelial growth factor cell proliferation and signal transduction) signal transduction pathway, and participates in the regulation of cell proliferation, differentiation, growth and other processes. In colorectal cancer patients, the BRAF gene mutation rate is about 15%. Clinical studies have found that the EGFR-targeting antibody Erbitux or panitumumab is ineffective against KRAS wild-type patients with BRAFV600E mutations, and no BRAF mutations were found in patients with effective treatment. When targeted drugs such as Erbitux or panitumumab are used, the KRAS gene status of the tumor tissue must be detected. If KRAS has no mutation, the BRAF gene status should be considered...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2531/113C12Q2535/137C12Q2545/101C12Q2563/107
Inventor 戴立忠吴康龙凤英高堂杰
Owner HUNAN SHENGWEI GENE TECH
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