The invention relates to a B-raf
gene mutation detection kit. The B-raf
gene mutation detection kit comprises quality-control primer probe internal-standard mixed liquor and detection primer probe internal-standard mixed liquor, wherein the quality-control primer probe internal-standard mixed liquor comprises a quality-control primer pair, a B-raf
gene specific probe, an internal-standard primer pair, an internal-standard specific probe and an internal-standard template, and the detection primer probe internal-standard mixed liquor comprises a B-raf gene V600E
mutation detection specific primer pair, a B-raf gene specific probe, an amplification blocking
nucleic acid sequence, an internal-standard primer pair, an internal-standard specific probe and an internal-standard template. The B-raf
gene mutation detection kit has the advantages that an amplification
refractory mutation
system is combined with a
wild type amplification blocking
nucleic acid sequence with a phosphorylated terminal, deoxyinosine is introduced into detection of a B-raf gene V600E
mutation detection specific ARMS (amplification
refractory mutation
system)
forward primer to enable quality-control PCR (
polymerase chain reaction) and detection PCR to be performed for detection of samples parallelly, and each
reaction system can have an internal-
standard system capable of effectively avoiding false negative and intra-
assay variation; the B-raf
gene mutation detection kit is low in cost, high in sensitivity and more capable of controlling intra-
assay and inter-
assay variation.