3 results about "V600E" patented technology

The invention provides application of an intracellular lactic acid level increasing agent in preparation of a drug for enhancing sensitivity of BRAF V600E mutant tumors to a BRAF inhibitor or in preparation of a drug for treating BRAF V600E mutant tumors resistant to the BRAF inhibitor. According to the invention, the intracellular lactic acid concentration is improved by using an intracellular lactic acid level improver, SUMOylation of BRAFV600E protein is promoted, and the BRAFV600E protein is locked in an open drug sensitive conformation; in-vivo and in-vitro experiments prove that the combination of the MCT4 inhibitor and the BRAF inhibitor (such as vimofenib) has a remarkable synergistic anti-tumor effect, drug resistance can be effectively relieved, and a new treatment strategy is provided for refractory colorectal cancer and the like.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-BRAF-V600E recombinant rabbit monoclonal antibody and application thereof, which comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application also relates to a nucleotide sequence, a recombinant plasmid or an expression vector, a preparation method and application of the anti-BRAF-V600E recombinant rabbit monoclonal antibody in a BRAF-V600E protein detection method or device. The BRAF-V600E recombinant rabbit monoclonal antibody of the 135K5D1 clone of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining, and is more accurate in detection and distinction of cancer.

The application discloses a primer probe composition for detecting a BRAF V600 gene mutation, a kit and application thereof. The application discloses a primer probe composition for detecting a BRAF V600 gene mutation, which comprises forward primers V600M-F and V600E-F shown in SEQ ID NO. 1 and SEQ ID NO. 2, a reverse primer V600R shown in SEQ ID NO. 3 and a detection probe V600P shown in SEQ ID NO. 4. Further disclosed are a kit containing the primer probe composition and application thereof. The primer probe composition is used for detecting a sample to be detected, has high sensitivity and specificity, can detect multiple BRAF V600 gene mutations, and can directly determine whether there is a mutation according to an amplification curve, without the need of calculating ΔCt, and is simple and direct.