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V600E is a mutation of the BRAF gene in which valine (V) is substituted by glutamic acid (E) at amino acid 600. It is a driver mutation in a proportion of certain diagnoses, including melanoma, hairy cell leukemia, papillary thyroid carcinoma, colorectal cancer, non-small-cell lung cancer, Langerhans cell histiocytosis, and ameloblastoma.
Primer probe composition, kit for detecting braf v600 gene mutation and application thereof
The application discloses a primer probe composition for detecting a BRAF V600 gene mutation, a kit and application thereof. The application discloses a primer probe composition for detecting a BRAF V600 gene mutation, which comprises forward primers V600M-F and V600E-F shown in SEQ ID NO. 1 and SEQ ID NO. 2, a reverse primer V600R shown in SEQ ID NO. 3 and a detection probe V600P shown in SEQ ID NO. 4. Further disclosed are a kit containing the primer probe composition and application thereof. The primer probe composition is used for detecting a sample to be detected, has high sensitivity and specificity, can detect multiple BRAF V600 gene mutations, and can directly determine whether there is a mutation according to an amplification curve, without the need of calculating ΔCt, and is simple and direct.
Owner:梅州市人民医院