B-raf gene mutation detection kit

Abstract

The invention relates to a B-raf gene mutation detection kit. The B-raf gene mutation detection kit comprises quality-control primer probe internal-standard mixed liquor and detection primer probe internal-standard mixed liquor, wherein the quality-control primer probe internal-standard mixed liquor comprises a quality-control primer pair, a B-raf gene specific probe, an internal-standard primer pair, an internal-standard specific probe and an internal-standard template, and the detection primer probe internal-standard mixed liquor comprises a B-raf gene V600E mutation detection specific primer pair, a B-raf gene specific probe, an amplification blocking nucleic acid sequence, an internal-standard primer pair, an internal-standard specific probe and an internal-standard template. The B-raf gene mutation detection kit has the advantages that an amplification refractory mutation system is combined with a wild type amplification blocking nucleic acid sequence with a phosphorylated terminal, deoxyinosine is introduced into detection of a B-raf gene V600E mutation detection specific ARMS (amplification refractory mutation system) forward primer to enable quality-control PCR (polymerase chain reaction) and detection PCR to be performed for detection of samples parallelly, and each reaction system can have an internal-standard system capable of effectively avoiding false negative and intra-assay variation; the B-raf gene mutation detection kit is low in cost, high in sensitivity and more capable of controlling intra-assay and inter-assay variation.

Description

technical field [0001] The invention relates to a gene mutation detection product, which belongs to the field of biotechnology. Background technique [0002] B-raf is an oncogene, which encodes a serine / threonine-specific kinase, an important transduction factor of the RAS / RAF / MEK / ERK / MAPK pathway, and participates in the regulation of various biological events in cells, such as Cell growth, differentiation and apoptosis, etc. B-raf mutation status is associated with the occurrence and development of various tumors. In a variety of human malignant tumors, such as malignant melanoma, colorectal cancer, lung cancer, thyroid cancer, liver cancer and pancreatic cancer, there are different proportions of B-raf gene mutations, about 66% of malignant melanoma and 15% of colon cancer There is a somatic missense mutation in the B-raf gene. About 80-90% of B-raf gene mutations occur at 1799 nucleotides of exon15, T is mutated to A, resulting in the substitution of glutamic acid enc...

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Problems solved by technology

However, the detection cost of this kit is too high, and each specimen needs 3000-5000 yuan, which is not suitable for widespread promotion

2) AmoyDx? BRAF V600E Gene Mutation Detection Kit is produced by Xiamen Aide Biomedical Co.,

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