Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer pair and probe combined product, composition, and kit used for detecting BRAF mutation, and applications thereof

A technology of combining products and primer pairs, applied in the field of molecular biology, can solve the problem of no LNA-MB detection method, etc., and achieve the effects of high accuracy, good reliability and high detection efficiency

Inactive Publication Date: 2018-08-07
求臻医学科技(浙江)有限公司
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no LNA-MB detection method for BRAF gene mutation in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer pair and probe combined product, composition, and kit used for detecting BRAF mutation, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] 1. Design of primers and probes

[0046] The wild-type and mutant gene sequences of human BRAF gene were obtained according to Genbank query, and allele-specific amplification primers and hydrolysis probes were designed with the software PrimerPremier5. The 3' end of the allele-specific upstream primer is a mutant base and uses a locked nucleic acid. The hydrolysis probe is labeled with FAM at the 5' end and BHQ1 at the 3' end.

[0047] (1) BRAF sequence

[0048] The partial sequence around the normal human BRAF V600E mutation is:

[0049] SEQ ID NO: 7

[0050] (2) Primers and probes

[0051] The common gene mutation of human BRAF is V600E, and the corresponding forward and reverse primers are shown in the table below.

[0052]

[0053] Wherein, the first nucleotide at the 3' end of SEQ ID NO: 3 / 4 is a locked nucleic acid;

[0054] The 13th and 20th nucleotides at the 5' end of SEQ ID NO: 1 are locked nucleic acids.

[0055] 2. Extraction of samples

[0056] 1....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of molecular biology, and more specifically relates to a primer pair and probe combined product, a composition, and a kit used for detecting BRAF mutation, and applications thereof. The combined product comprises a LNA fluorescence probe and primers used for detecting BRAF gene V600E mutation; wherein the forward primer sequence of the primer pair comprises at least one locked nucleic acid, and / or, the LNA fluorescence probe sequence comprises at least one locked nucleic acid. The primer pair and the probe composition is rapid and convenient in detection, ishigh in specificity, sensitivity, reliability, detection efficiency, and accuracy, low in detection cost, short in detection time, and low in false positive rate. The kit can be used for detecting BRAF mutation, is capable of providing guidance for clinical diagnosis and treatment of BRAF mutation cancer, and is promising in application prospect.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a combined product, composition, kit and application of a primer pair and a probe for detecting BRAF mutation. Background technique [0002] Molecular targeted therapy is one of the hot spots in the field of cancer treatment research, and epidermal growth factor receptor inhibitors are a class of targeted therapy drugs. Studies have shown that there is a clear correlation between the mutation status of the human BRAF gene and the efficacy of epidermal growth factor receptor inhibitors. [0003] Circulating tumor DNA (ctDNA) is a new type of tumor molecular marker, which has the characteristics of high detection rate, specificity, and high consistency with tissue detection results. ctDNA can be used to detect tumors in a non-invasive manner, which is helpful for clinical judgment of KRAS gene mutation status in tumor tissues. However, due to the low content of circulating free D...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6858
CPCC12Q1/6858C12Q2525/117C12Q2563/185C12Q2563/107
Inventor 段小红承康平杨春燕丁蕊王东亮周启明
Owner 求臻医学科技(浙江)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com