32results about How to "The result is intuitive and clear" patented technology

This invention relates to a method of detection of food-borne pathogenic bacteria using multiple fluorescent PCR and modified molecular beacons. Several pairs of primers and modified molecular beacon probes are designed according to the specific gene sequence of the pathogenic bacteria to be tested, which is amplified using fluorescent PCR and modified molecular beacon. The amplified product is crossbred with molecular beacon, and the fluorescent intensity of the reaction system is tested with the necessary circle times for intended threshold as a judgment for results, that is, negative for Ct of 0 or 40 and positive for Ct less than 38. This invention, which is advanced in high sensibility, significant specificity, simple operation and intuitionistic observation, is applicable in simultaneous detection of any two kinds, three kinds and four kinds of randomly combined bacteria and food-borne pathogenic bacteria as well as specimen in large amounts.

The graphical representation method operate topological structure of network nodes includes arranging son nodes in some circumference in fixed radius and with root node as circle center to divide the circumference into several sectors, each of which corresponds to a son node set in the angular bisector, weight averaging and calculating size of sector angles with the weight value being the leaf node number of son tree with corresponding son node as root, and so on to position sublevel son nodes and to realize annular distribution graph. The graphical representation of the present invention is intuitive, clear, homogeneously distributed and high in system running efficiency.

The invention provides a detection kit for virulence gene of spinal muscular atrophy and application thereof, comprising primer SMN-Ex7-F with such sequences as SEQ ID NO.1; primer SMN-Ex7-R with such sequences as SEQ ID NO.2 and probe SMN-Ex7-P with such sequences as SEQ ID NO.3. The above kit in the invention can be applied to detect the carrier of spinal muscular atrophy virulence gene, comprising the steps of extracting the genome DNA from subject to be detected; precisely quantifying the genome DNA from subject to be detected; and performing primary fluorescent quantitation PCR based on genome DNA from subject to be detected as the template to obtain the primary fluorescent quantitation curve. The kit in the invention can visually, precisely, quickly and succinctly detect the SMN1 gene, so as to realize the distinguishing of SMA virulence gene carrier and normal people.

The invention provides an H.265 ultra-high definition video quality evaluation method. The method comprises the following steps of performing YUV sampling on the original video to obtain an original video sequence; performing video quality evaluation on the original video sequence and the test video sequence, and calculating video quality parameters; and obtaining an evaluation result by the unified video quality evaluation parameters according to the video quality parameters. The method has the advantages that the video quality of the ultra-high-definition video above 4K can be evaluated, andthe limitation of the current video quality evaluation method on the video resolution is broken through.

The present invention relates to a mine pressure signal real-time visual demodulation system and demodulation method based on optical fiber sensing. The demodulation system is mainly formed by a mine pressure signal acquisition subsystem, an optical signal transmission subsystem, a photoelectric signal conversion subsystem and a digital signal visual processing subsystem. A mine pressure signal is collected through arranging an optical fiber sensor in a coal mine, the mine pressure signal is transmitted to an optical fiber grating demodulator for a mine in the form of optical waves and is demodulated into wavelength data, the digital signal visual processing subsystem demodulates the wavelength data into a mine pressure physical quantity and automatically outputs a visual mine pressure chart and a mine pressure monitoring report. The system and the method have the advantages that the demodulation system is comprehensive and diversified, the development performance is high; the system equipment is essentially safe and is especially suitable for a complex underground environment; the demodulation algorithm is professional, and the mine pressure demodulation is more accurate; a demodulation result is clear and visual, and the degree of visualization is high; the system response is timely and effective, the underground mine pressure can be displayed in real time, and the safe and efficient production of a coal mine is ensured.

The invention discloses a substation grounding grid impact diffusion characteristic test device which comprises an impact current generator (10) which is connected to grounding grid (30) impact current injection points by using a conductive wire (20), a plurality of open-close type current sensors (40) which are distributed at the intersection points of a plurality of circles with the impact current injection points as centers and the change lengths of equidifferent change as radii, a measurement system (60) which is connected to the plurality of open-close type current sensors (40) through a signal cable (50), a monitoring system (70) which comprises a laptop computer (701) and a wireless router (702) which are interconnected, and an insulation device (80) which comprises an insulating pad (802) arranged under the measurement system and epoxy tubes (801) which are used as the connection wires between devices and the brackets of the signal cable. According to the device, the error in a measurement process can be effectively reduced, and the efficiency of a field test is improved.

The invention relates to a test paper for quickly determining peracetic acid disinfection liquid and a preparation method, and belongs to the field of sanitary disinfection medicines. The test paper is characterized in that the test paper is treated by a solution containing potassium iodide and a stabilizer, and the mass ratio of sodium thiosulfate to sodium carbonate to polyvinyl alcohol in the solution is 1:(0.5 to 5):(0.25 to 2.5). The preparation method comprises the following steps of completely soaking the test paper into mother liquid, soaking for 3 to 5min, fetching out, drying at the temperature of 50 to 70 DEG C, and cutting into proper sizes after the test paper is completely dried, so as to obtain the test paper. The operation method has the advantages that the operation is simple and convenient, the result is clear and visual, the clinical requirement on the concentration and accuracy of the peracetic acid disinfection liquid is completely met, any special technical requirement on an operator is avoided, and the disinfection result of the used peracetic acid disinfection liquid can be visually and quickly evaluated.

The invention discloses a kit and a method for simultaneous detection of a Staphylococcus aureus gene and an Escherichia coli gene by using a dual molecular beacon-LAMP process, belonging to the technical field of molecular biological detection. The kit comprises an LAMP reaction system, wherein the LAMP reaction system comprises a primer group used for detecting the Staphylococcus aureus gene nuc and an rfbE gene of the Escherichia coli O157: H7, the primer group includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the nuc gene and further includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the rfbE gene. The method provided by the invention has the advantages of high sensitivity, strong specificity, avoidance of cross reaction with a dozen of bacteria of a control group, simple operation, realization of visual and clear observation of results and a fast detection speed.

The invention relates to a partial discharge monitoring and positioning device and method, computer equipment and a storage medium. The partial discharge monitoring and positioning device comprises ameasuring unit and a control unit, the measuring unit comprises an image acquisition sensor and a sonic sensor array, and the image acquisition sensor and the sonic sensor array are both in communication connection with the control unit; and the control unit is used for receiving the measurement data, acquired by the image acquisition sensor and the sonic sensor array, of to-be-measured equipment,and calculating and determining the partial discharge position of the to-be-measured equipment according to the measurement data. The image data of the to-be-measured equipment is acquired through the image acquisition sensor, the sound wave data generated by the to-be-measured equipment is received through the sonic sensor array, the control unit can accurately position the partial discharge position according to the image data and the sonic wave data and display the partial discharge position in combination with the image, and the result is more intuitive and clearer compared with infrareddetection.

The invention discloses a comprehensive evaluation method for urban traffic sustainable development, which belongs to the field of urban traffic transportation system development evaluation, and comprises the following steps: (1) constructing an evaluation index system according to a TISP framework, and grading evaluation results; (2) determining a grading standard of the index according to the related national standard and the city scale; (3) calculating digital features of the cloud model by combining a multi-dimensional cloud model theory according to each index grading standard; (3) inputting digital characteristics of the cloud model, and generating a normal random number corresponding to the evaluation level by a multi-dimensional forward normal cloud generator; (4) calculating an index weight by adopting an AHP method; And (5) inputting actual measurement values of urban traffic system indexes, calculating the certainty degree belonging to each level by utilizing a comprehensivecertainty degree formula, and judging the comprehensive evaluation level according to a maximum certainty degree principle. The method is simple in step, efficient in calculation and clear in result,and has important reference significance for sustainable development evaluation of the urban transportation system.

The invention discloses a bus temperature grading warning device. The bus temperature grading warning device comprises a temperature detection module, a temperature sensor is disposed in the temperature detection module, an output end of the temperature detection module is connected with a comparison circuit, an output end of the comparison circuit is connected with an indicating lamp module via an amplification circuit, and an output end of the amplification circuit is connected with an alarm device; and an output end of the temperature detection module is further connected with a signal emitter, and the signal emitter is in communication with an intelligent terminal through a Zigbee data transmission unit. The device can visually reflect a bus temperature condition on a bus cabinet through signal lamps and the alarm device and also can check a temperature condition of a bus through the intelligent terminal, a result is visual and clear, the device helps on-site operation personal rapidly find a bus at over-high temperature, and a corresponding measure is taken; and the structure is simple, wiring and installation are convenient, and overall cost and power consumption are reduced.

The invention belongs to the technical field of concrete durability monitoring. The invention particularly relates to an ECT sensor structure optimization and electromagnetic field analysis method. The technological process comprises eight steps including parameter setting, geometric setting, material setting, mesh generation, physical field setting, solving, sensor structure optimization and electromagnetic field distribution calculation. A new idea is provided for solving the positive problem of an ECT system based on software COMSOL. After modeling is completed, the uniformity degree of thesensitive field of an ECT sensor is analyzed through a calculation result, structure parameter values of all parts of the ECT sensor are adjusted, an optimal design scheme is searched for, meanwhile,change of the internal electromagnetic field when a concrete structure contains water can be visually and clearly observed, the relation between the internal moisture content of the concrete structure and the relative dielectric constant is obtained, the accuracy of imaging the internal moisture content of the concrete structure through the ECT technology is improved, and a new verification method is provided for the durability test of the concrete structure.

The invention discloses a cylinder plate method-based leather material bacteriostasis effect test method. The test method includes the following steps: (1) sample preparation; (2) 2,3,5-triphenyltetrazolium chloride solid medium preparation; (3) 2,3,5-triphenyltetrazolium chloride semisolid medium preparation; (4) inoculant suspension preparation; (5) testing operation; (6) evaluation and reporting. The cylinder plate method-based leather material bacteriostasis effect test method solves the defects of small bacterial colony, nontransparent sample and uneasy observation and recording; the test method is easy to operate, the result is accurate, the repeatability is good, and the test method has a high application value.

The graphical representation method operate topological structure of network nodes includes arranging son nodes in some circumference in fixed radius and with root node as circle center to divide the circumference into several sectors, each of which corresponds to a son node set in the angular bisector, weight averaging and calculating size of sector angles with the weight value being the leaf node number of son tree with corresponding son node as root, and so on to position sublevel son nodes and to realize annular distribution graph. The graphical representation of the present invention is intuitive, clear, homogeneously distributed and high in system running efficiency.

The invention discloses a self-adaptive target detection method and device based on an event camera. The method comprises the steps of: selecting initial time and event reconstruction interval time, enabling all events in the event reconstruction interval time range to form an event window, obtaining a mean value time graph corresponding to the event window, and initializing the center point position and side length of a self-adaptive target frame; and drawing an initial target frame in the mean value time graph, iteratively calculating the mean value and the standard deviation of all event point positions in the current target frame, and updating the target frame until an iteration termination condition is met. The method is simple in implementation process and low in computing power requirement, and an ideal effect can be achieved on a lightweight platform.

The invention belongs to the field of oil and natural gas industry oil casing thread parameter detection, and particularly relates to a method for judging the angle of an oil casing knuckle thread form. The method comprises the following steps that (1) after casing thread machining is completed, a thread form height deviation value h is measured; (2) a judging criteria is set to be h<k(H-Ss-(p / 2) / tan(A / 2)); and (3) when the measured thread form height deviation value h meets the judging criteria, the angle of the thread form is judged to be qualified, otherwise, the angle of the thread form isjudged to be unqualified. According to the method for judging the angle of the oil casing knuckle thread form, the judging range is obtained through mathematic model calculation, judging is conductedby comparing the measurement value with the judging range, the result is intuitive and clear, and the detection efficiency is high.

The invention provides an evaluation method and device for the boiler shutdown protection anti-corrosion effect of a thermal power generating unit. Operation is easy, implementation is easy, the anti-corrosion condition in a huge coal-fired boiler water vapor system pipeline in the boiler shutdown protection state can be effectively mastered, and whether the currently-adopted coal-fired boiler shutdown protection method is reliable and effective or not is judged. The evaluation method for the boiler shutdown protection anti-corrosion effect of the thermal power generating unit comprises the steps that the accumulated flow of a condensate pump outlet in the unit starting stage is measured; the mass content of iron in the water vapor related to the boiler heating surface in the unit starting stage is measured, and calculation is performed to obtain the average value of the iron content in the water vapor related to the boiler heating surface; according to the accumulated flow and the iron content average value, the total iron content of the unit in the starting stage is calculated through a preset total iron amount fitting formula; and the total iron content is used as an evaluation index to evaluate the blowing-out protection anti-corrosion effect of the unit before the unit is started at the time.

The invention provides a power distribution area electric vehicle load identification method based on blind signal separation, and belongs to the technical field of power distribution network management and control. The problem that the access of the charging equipment cannot be identified due to unknown topology and information loss in the power distribution area is solved. According to the technical scheme, the method comprises the following steps: 1) establishing an electric vehicle load model; 2) a blind signal separation model; and 3) constructing a membership distance and membership degree function. The method has the beneficial effects that the electric vehicle load model and the blind signal separation model are established, the non-linear load of the electric vehicle is processed in a separation and non-linear mapping mode, a non-linear signal is converted into a linear signal, the separated result is more accurate, the speed is higher, and the blind signal is more accurate. And the precision of the separation result of blind signal separation is high.

The invention discloses a device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The device comprises a test platform, the test platform comprises a base layer, a test layer and a feed layer, the test layer is composited between that base layer and the feed layer, a feeding hole is arranged on the feed layer, a testing area is arranged on the testing layercorresponding to the position of each feeding hole, the testing area is isolated from the blank position on the testing layer through a first partition structure, a second partition structure is arranged between each testing area and other adjacent testing areas, and partitions are arranged in the first partition structure and the second partition structure. The invention also discloses a preparation method of the device, and also discloses a detection method for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The detection device of the invention has simple structural design and convenient carrying; the preparation method of the detection device is simple and the cost is low; the detection method of the invention can simply test the minimum inhibitoryconcentration of antibiotics, and the result is accurate.

The invention relates to the technical field of information processing, in particular to a standard analysis method and system. The method comprises the following steps that: storing standard information data according to a standard attribute, and updating the standard information data in real time; according to the standard attribute, retrieving and downloading the standard information data; analyzing the retrieved and downloaded standard information data according to standard characteristics to obtain an analysis result, and carrying out error correction on the analysis result; and displaying and storing the analysis result subjected to error correction. By use of the method, on the basis of existing standard data, analysis is carried out to find a standard formulation blank point to assist an enterprise in formulating a standard layout strategy.

The invention provides an H.265 ultra-high definition video quality evaluation method. The method comprises the following steps of performing YUV sampling on the original video to obtain an original video sequence; performing video quality evaluation on the original video sequence and the test video sequence, and calculating video quality parameters; and obtaining an evaluation result by the unified video quality evaluation parameters according to the video quality parameters. The method has the advantages that the video quality of the ultra-high-definition video above 4K can be evaluated, andthe limitation of the current video quality evaluation method on the video resolution is broken through.

This invention relates to a method of detection of food-borne pathogenic bacteria using multiple fluorescent PCR and modified molecular beacons. Several pairs of primers and modified molecular beacon probes are designed according to the specific gene sequence of the pathogenic bacteria to be tested, which is amplified using fluorescent PCR and modified molecular beacon. The amplified product is crossbred with molecular beacon, and the fluorescent intensity of the reaction system is tested with the necessary circle times for intended threshold as a judgment for results, that is, negative for Ct of 0 or 40 and positive for Ct less than 38. This invention, which is advanced in high sensibility, significant specificity, simple operation and intuitionistic observation, is applicable in simultaneous detection of any two kinds, three kinds and four kinds of randomly combined bacteria and food-borne pathogenic bacteria as well as specimen in large amounts.

The invention discloses application of a protease graph electrophoresis detection method to rapid identification of extracellaluar protease variety of bacteria. The application is implemented by the following steps: a, preparing SDS (sodium dodecyl sulfate) polyacrylamide gel containing no a protease substrate; b, evenly mixing a protease sample with a loading buffer solution containing no beta-mercaptoethanol and then loading the sample without heating in boiling water bath; c, carrying out electrophoresis; d, washing the gel; e, soaking the gel in a substrate solution containing different protease inhibitors; and f, reacting and developing. The protease graph electrophoresis detection method is capable of determining the natures of different proteases and obviously shortened in time in contrast with a traditional method; complex steps of separating and purifying the proteases are omitted; the method is good in repeatability, high in sensitivity and accurate in active stripe measurement, thus being highly advantageous for subsequent protease identification and purposeful purification operation; the method can be applied to detection of various types of proteases and has wide use space.

The invention discloses a kit and a method for simultaneous detection of a Staphylococcus aureus gene and an Escherichia coli gene by using a dual molecular beacon-LAMP process, belonging to the technical field of molecular biological detection. The kit comprises an LAMP reaction system, wherein the LAMP reaction system comprises a primer group used for detecting the Staphylococcus aureus gene nuc and an rfbE gene of the Escherichia coli O157: H7, the primer group includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the nuc gene and further includes a pair of outer primers F3 and B3, a pair of inner primers FIP and BIP and a molecular beacon probe of the rfbE gene. The method provided by the invention has the advantages of high sensitivity, strong specificity, avoidance of cross reaction with a dozen of bacteria of a control group, simple operation, realization of visual and clear observation of results and a fast detection speed.

The invention relates to a special detection device for detecting the double-hole concentricity of a sewing machine positioning block. The special detection device comprises a base plate, a supporting base, a workpiece positioning rod and a workpiece detection concentric rod, and is characterized in that the bottom of the supporting base is hinged on the base plate through a rotating shaft, the upper end of the supporting base is provided with a groove used for placing a workpiece, the workpiece positioning rod is inserted at one side of the supporting base, the front end of the workpiece positioning rod extends into the groove, the workpiece detection concentric rod is inserted at the other side of the supporting base, and the workpiece detection concentric rod and the workpiece positioning rod are coaxially arranged. The special detection device for detecting the double-hole concentricity of the sewing machine positioning block is ingenious in design, simple in structure and convenient to use, and application of the device can simplify the detection process, accelerate the detection speed and improve the accuracy of a detection result.

The invention discloses medical rapid detection electrocardiogram equipment. The medical rapid detection electrocardiogram equipment comprises a detector body, a power supply, an electrode, a lead wire, a display screen, a switch, an enter key, a return key, a navigation key and a testing key, wherein the electrode is connected with the detector body through the lead wire; and the power supply, the display screen, the switch, the enter key, the return key, the navigation key and the testing key are arranged on the detector body. A data line slot is formed in the top end of the detector body of the medical rapid detection electrocardiogram equipment; the detector body is made of a plastic material; and the power supply can be rechargeable battery. The circumstance that a detection mode in the prior art is troublesome and complicated is changed, so that electrocardiograph detection is simple, and the result is clear and visual; the integral structure is simple and small, so that the medical rapid detection electrocardiogram equipment is convenient to carry when family members and doctors go out; and the medical rapid detection electrocardiogram equipment is high in practicality and easy to popularize and use.

This invention relates to a method of detection of food-borne pathogenic bacteria using double fluorescent PCR and modified molecular beacons. Corresponding pair of primers 1 and a modified molecular beacon probe 1 and another pair of primers 2 and another modified molecular beacon probe are designed according to the specific gene sequence of the two pathogenic bacteria to be tested, which is amplified using fluorescent PCR and modified molecular beacon. The amplified product is crossbred with molecular beacon, and the fluorescent intensity of the reaction system is tested with the necessary circle times for intended threshold as a judgment for results, that is, negative for Ct of 0 or 40 and positive for Ct less than 38. This invention, which is advanced in high sensibility, significant specificity, simple operation and intuitionistic observation, is applicable in simultaneous detection of any two kinds of randomly combined bacteria and food-borne pathogenic bacteria as well as specimen in large amounts.

The invention relates to a method for optimization of activated carbon and establishes a comprehensive evaluation model of granular activated carbon. The comprehensive evaluation model has an expression formula shown in the description, wherein Ii represents the comprehensive score of alternative granular activated carbon i, Wj represents a weight coefficient of the j parameter, Aij represents themembership degree of the j parameter of the alternative granular activated carbon i, and j=1, j=2, j=3, j=4 , j=5 and j=6 respectively correspond to the iodine adsorption value, methylene blue adsorption value, UV254 average removal rate, DOC average removal rate, strength and unit volume (or unit mass) price of the alternative granular activated carbon. The granular activated carbon with the largest Ii value is selected. Compared with the prior art, the method comprehensively considers the static adsorption performance, dynamic adsorption performance, strength and cost of the granular activated carbon, comprehensively evaluates various alternative granular activated carbon, selects according to the comprehensive evaluation score and produces clear and intuitive results.