Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof

A technology of inhibitory concentration and detection method, applied in the field of microbial detection, can solve the problems of high price, insufficient reproducibility and quantification, cumbersome experimental process, etc. Accurate experimental results

Pending Publication Date: 2019-12-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The agar dilution method is the standard method of WHO, which has a long cultivation period and cumbersome experimental process
Disc diffusion method is expensive and lacks reproducibility and quantitation
The drug res

Method used

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  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof
  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof
  • Device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae, preparation method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1

[0043] A device for rapidly detecting the minimum inhibitory concentration of Neisseria gonorrhoeae, including a test platform, such as image 3 As shown, the test platform includes a base layer 1, a test layer 2 and a feeding layer 3. The test layer 2 is compounded between the base layer 1 and the feeding layer 3. The feeding layer 3 is provided with feeding holes 4, and the test layer 2 corresponds to each feeding layer. The position of the hole 4 is provided with a test area, the test area is separated from the blank position on the test layer by the first partition structure 5, and a second partition structure 6 is provided between each test area and other adjacent test areas, such as figure 2 Shown; the first partition structure 5 and the second partition structure 6 are provided with partitions, such as image 3 Shown.

[0044] The setting of the partition structure can isolate each part into a separate area without interfering with each other, ensuring the...

Example Embodiment

[0055] Example 2

[0056] The present invention also provides a preparation method of a device for rapidly detecting the minimum inhibitory concentration of Neisseria gonorrhoeae. The preparation method is used to prepare the above-mentioned device and includes the following steps:

[0057] (1) Use CorelDRAW to draw and design the shapes and patterns of the base layer, the test layer and the feed layer, and use the printer to print out the designed shapes and patterns, cut them, and divide them into the first partition structure, the second partition structure and the third partition structure. The tank is filled with partitions;

[0058] (2) Wrap the test platform with tin foil, place it on a hot plate, and heat it at 160°C for 3 minutes. After the wax melts, place it under an ultraviolet lamp and irradiate the front and back for 30 minutes;

[0059] (3) Configure ceftriaxone standard solutions with different concentration gradients; the concentrations of ceftriaxone standard solutio...

Example Embodiment

[0065] Example 3

[0066] The present invention also provides a detection method for rapidly detecting the minimum inhibitory concentration of Neisseria gonorrhoeae. The detection method adopts the device prepared by the above preparation method, and includes the following steps:

[0067] 1) Configure Neisseria gonorrhoeae suspension;

[0068] ① Scrape an appropriate amount of Neisseria gonorrhoeae with a bacterial ring. This Neisseria gonorrhoeae was purchased from the American Type Culture Collection ATCC with the preservation number ATCC49226;

[0069] ②Put Neisseria gonorrhoeae in a 1.5mL centrifuge tube, add 1mL PBS buffer and mix well for testing.

[0070] ③Use a pipette to take 200ul of the Neisseria gonorrhoeae suspension and PBS buffer solution obtained in step ② in a 96-well plate, measure the optical density values ​​of both with a spectrophotometer, and dilute the Neisseria gonorrhoeae suspension to a concentration of 10 -8 cfu / mL;

[0071] 2) Place the test platform in a pet...

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Abstract

The invention discloses a device for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The device comprises a test platform, the test platform comprises a base layer, a test layer and a feed layer, the test layer is composited between that base layer and the feed layer, a feeding hole is arranged on the feed layer, a testing area is arranged on the testing layercorresponding to the position of each feeding hole, the testing area is isolated from the blank position on the testing layer through a first partition structure, a second partition structure is arranged between each testing area and other adjacent testing areas, and partitions are arranged in the first partition structure and the second partition structure. The invention also discloses a preparation method of the device, and also discloses a detection method for rapidly detecting the minimum bacteriostatic concentration of Neisseria gonorrhoeae. The detection device of the invention has simple structural design and convenient carrying; the preparation method of the detection device is simple and the cost is low; the detection method of the invention can simply test the minimum inhibitoryconcentration of antibiotics, and the result is accurate.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, and in particular relates to a device, preparation and detection method for rapidly detecting the minimum inhibitory concentration of gonorrhoeae. Background technique [0002] Neisseria gonorrhoeae is a relatively common sexually transmitted pathogenic microorganism in clinical practice, which is highly infectious to humans. Neisseria gonorrhoeae mainly causes purulent infection of the genitourinary system, and can also cause combined or disseminated infections such as gonococcal ophthalmia, pharyngitis, proctitis, and pelvic inflammatory disease. An estimated 78 million new cases of N. gonorrhoeae occur each year in people aged 15-49 years. Up to now, gonorrhea caused by Neisseria gonorrhoeae has neither effective vaccine nor long-lasting immunity after being cured. As an infectious disease, it has been endangering human health. In the treatment of gonorrhea, the drug resistance o...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00C12Q1/18
CPCC12Q1/18
Inventor 王书崎王怡敏梁利国
Owner ZHEJIANG UNIV
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