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76results about How to "The result is intuitive and accurate" patented technology

Optical rotation angle field detection device based on polarized beam splitting imaging and measurement method thereof

The invention discloses an optical rotation angle field detection device based on polarized beam splitting imaging and a measurement method thereof. The device comprises a polarized light generating device, a polarized beam splitting device, a light receiving and imaging device, an image acquisition device and a data processing system, wherein the polarized light generating device emits a bundle of linearly polarized light; the linearly polarized light is vertically incident to the polarized beam splitting device and forms two bundles of linearly polarized light after emergence from the polarized beam splitting device; the two bundles of light are respectively received by the light receiving and imaging device; and then, the light intensity information of the two bundles of light is transferred to the data processing system through the image acquisition device and is processed by the data processing system to obtain an optical rotation angle field. In the invention, the detection process is free from manual adjustment, the operation is simple, and errors caused by manual adjustment can be avoided; a two-dimensional field in a polarization state can be obtained directly by adoptingan imaging and image processing algorithm, the detection result is more visual and accurate and is free from the effect of light source fluctuation.
Owner:NANJING UNIV OF AERONAUTICS & ASTRONAUTICS

Method for determining non-linear HIFU (High Intensity Focused Ultrasound) sound filed of biological tissues

The invention discloses a method for determining the non-linear HIFU (High Intensity Focused Ultrasound) sound field of biological tissues, which belongs to the technical field of sound field measurement. The method mainly comprises the following steps of: (a) transmitting a signal through a pulse receiving-transmitting instrument; (b) calibrating an energy transducer; (c) exciting a dual-frequency ultrasonic signal in a sample to be tested; (d) receiving the signal, sampling at equal intervals on different space and time domains, and performing FFT (Fast Fourier Transform) processing on the signal; (e) calculating the attenuation and dispersion of a sample material, smoothing a signal windowed function, and processing the signal by using two-dimensional Fourier transform to obtain the dispersion and attenuation spectrum of an ultrasonic signal; and (f) simulating the sound field in the sample by using a computer through a dispersion and an attenuation value obtained in the step (e). Due to the adoption of the method, the sound wave condition of sound filed transmitted in the biological tissues can be determined really and accurately; and the method has the characteristics of simple measuring method and high result precision.
Owner:NANJING GUANGCI MEDICAL TECH

Genetic relationship identification method with SNP as genetic marker

The invention relates to the technical field of genetic relationship identification methods, and discloses a genetic relationship identification method with SNP as a genetic marker. The method comprises the following three steps of: establishing a genetic relationship judgment model, carrying out typing detection on SNP sites, and confirming the genetic relationship among individuals according toa typing result and the judgment model. According to the genetic relationship identification method with the SNP as the genetic marker, the SNP is used as the genetic marker in replacement of STR in the detection mode, so the defects of poor stability, high typing difficulty, high sample quality requirements and high cost when the STR is used as the genetic marker for detection are fully avoided;whether two samples are of a direct line, or are in a first-level genetic relationship, a second-level genetic relationship or a third-level genetic relationship, or are strangers can be directly judged through a new model algorithm; a probability density model is introduced, so the influence caused by various errors is fully avoided; programmed judgment is adopted, so operation is easy and convenient; an SNP typing detection technology is adopted, so typing results are accurate and visual; and a great number of SNP sites are used by the algorithm, the number of repetitions is high, and the result is more accurate.
Owner:北京奇云诺德信息科技有限公司

Evaluation method of in-vitro natural killer cell immunocompetence and application thereof

The invention provides an evaluation method of in-vitro natural killer cell immunocompetence and application thereof. The evaluation method comprises the following steps of: co-culturing a target cellcarrying a fluorescence label A and a natural killer cell, dyeing and labelling dead cells generated after co-culture by using a fluorescence label B, and carrying out microscopic imaging on the dyedand labelled cells by using a bright field, a fluorescence channel matched with the fluorescence label A and a fluorescence channel matched with the fluorescence label B respectively so as to obtaina microscopic image, recognizing cells in the obtained microscopic image through image recognition, performing superposition synthesis analysis on recognition results of the same region, and evaluating the immunocompetence of the natural killer cells according to an analysis result. According to the invention, a direct reading method for directly obtaining a detection result through an image is adopted, so that the detection result is more accurate and visual; and thus, the method is suitable for the fields of medical diagnosis and biomedical industrialization with higher standardization requirements.
Owner:SHANGHAI RUIYU BIOTECH

Real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for detection of foot and mouth disease virus and seneca valley virus and application

The invention discloses a real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for detection of foot and mouth disease virus and seneca valley virus and application. The kit comprises primers and probes for detecting the foot and mouth disease virus and the seneca valley virus and preferably further comprises nucleic acid extract liquid, 2*Direct qRT-PCRMix, enzyme mixed liquid, negative control and positive control. By adoption of the kit for detecting the foot and mouth disease virus and the seneca valley virus, high specificity, high sensitivity,high stability, simplicity and convenience in operation and the like are achieved. Without extra extraction of virus RNA and reverse transcription, a user only needs to add a to-be-tested sample intoa reaction tube, then performs quantitative analysis on a start template according to fluorescence signal changes and a Ct value and standard curve relation and finally calculates a copy number of the to-be-tested sample. The kit is not only applicable to quantitative analysis in research and development institutions but also suitable for pathogen detection and analysis in all levels of prevention and control institutions, basic veterinary stations, large and medium sized farms and the like, thereby having a promising application prospect.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI

PCR primer set, site, method and kit for minimally invasive and rapid identification of ZW sex chromosome type of pelodiscus sinensis

The invention discloses a PCR primer set, site, method and kit for minimally invasive and rapid identification of the ZW sex chromosome type of pelodiscus sinensis. The sequence of the primer set is shown as SEQ ID No.1 and SEQ ID No.2. With a trace amount (0.5-1 microliter) of pelodiscus sinensis blood as a starter template, the primer set is used for carrying out PCR amplification, an amplification product is subjected to electrophoresis detection, and when 2031 bp bands appear in the amplification product, it is determined that the pelodiscus sinensis is in a ZZ sex chromosome type; when 2031 bp and 1624 bp bands appear in the amplification product, it is determined that the pelodiscus sinensis is in a ZW sex chromosome type. With the trace amount of blood obtained by minimally invasivecollection as a starter material, the sex chromosome type of the pelodiscus sinensis is rapidly detected; the detection hardly damages tested individuals, it can be ensured that pelodiscus sinensis in each growth and development stage can survive healthily after sampling, and an important tool is provided for identifying the sex hromosome type in the pelodiscus sinensis monosex culture technologyand sex-controlled breeding.
Owner:ZHEJIANG FISHERIES TECH EXTENSION STATION

Paper chip for rapidly detecting organophosphorus pesticide and production method thereof

The invention discloses a paper chip for rapidly detecting organophosphorus pesticide. The paper chip comprises test paper, an electrode, an immobilized reaction enzyme and a gold finger interface connected with the electrode, wherein a hydrophobic layer is selectively coated on the surface of the test paper to reserve a reaction area uncoated by the hydrophobic layer and a sample feeding channel on the surface of the test paper; a cofferdam formed by the hydrophobic layer is arranged around the reaction area; the sample feeding channel is led to the reaction area from the edge of the test paper; the detection end of the electrode is arranged in the reaction area; the other end of the electrode is connected with the gold finger interface formed in the end of the test paper; the immobilized reaction enzyme is coated on a junction of the reaction area and the sample feeding channel. When the paper chip is matched with a special detection instrument, the paper chip is capable of reflecting whether residues in a detected solution exceed the standard through direct degrees of the detection instrument according to the potential change generated by a reaction of the reaction enzyme and the detected solution; the paper chip is simple, convenient and rapid to operate, intuitive and accurate in the results and low in the cost; the test paper can be used without the influence of light and environment; the test paper can be taken together with a handheld detection device to carry out rapid detection outdoors.
Owner:SUZHOU GST INFOMATION TECH CO LTD

High-precision detection method for seismic signal coherence

The invention discloses a high-precision detection method for seismic signal coherence, wherein the high-precision detection method is improved on the basis of a Gaussian kernel function and a higherderivative. On the basis of an eigenvalue coherence algorithm, also called as a third generation coherence algorithm, a third generation coherent data volume is convoluted with the Gaussian kernel function, a second-order partial derivative of the obtained new data volume on a main line and a crossline and in time direction is solved, maximum eigenvalue of data after derivation is solved, and themaximum eigenvalue is taken as a final result of an improved algorithm, so that a new coherent result is obtained. The high-precision detection method disclosed by the invention is used for detectingsimilarity, namely coherence, of a seismic signal, so that the high-precision detection method is applied to identification and prediction of a subsurface fault, a fracture-developed zone and some sedimentary characteristics; meanwhile, the high-precision detection method disclosed by the invention can more clearly show spatial distribution of a fault and reflect detail characteristics of a subsurface structure and a sedimentary environment, so that a new promoting effect is produced to scientific research.
Owner:CHENGDU UNIVERSITY OF TECHNOLOGY

Primers and method for detecting related gene SNP (single nucleotide polymorphism) sites of metformin-individualized medications

The present invention relates to primers and a method for detecting related gene SNP (single nucleotide polymorphism) sites of metformin-individualized medications. The primers can detect a rs2289669SNP site of a SLC47A1 gene. The primer sequences comprise a wild-type upstream primer, a mutant-type upstream primer and a shared downstream primer. Improvement is made on the basis of ARMS-PCR, a specific primer and the shared downstream primer are designed for SNP sequences, mismatched bases are introduced at a second position of a 3' end of the specific primerto widen a gap between amplification efficiency of complementation and non-complementation of the bases of the 3' end of the specific primer and SNP site bases on a DNA template, thereby greatly improving specificity and accuracy of detection. A real-time PCR technology is introduced, identification can be made according to a [delta]Ct value, that is, the [delta]Ct value is equal to mutant Ct-wild Ct. In general, when [delta]Ct islarger than 7, the SNP site is a wild type, when [delta]Ct is less than -7, the SNP site is a mutant type, and when the [delta]Ct is larger than -2 and less than 2, the SNP site is a heterozygous type. Advantages are that the method is fast, simple, economical, accurate and reliable identification of results, and high in specificity.
Owner:西安医臻生物医药科技有限公司

Method for identifying quality difference of thin-skinned melon fruits after application of forchlorfenuron

The invention relates to the technical field of analysis and detection, and provides a method for identifying the quality difference of thin-skinned melon fruits after application of forchlorfenuron.The method comprises the following steps of detecting and analyzing volatile organic compounds in a thin-skinned melon fruit sample after application of forchlorfenuron based on a headspace-gas chromatography-ion mobility spectrometry technology, and determining a characteristic peak comparison diagram corresponding to the volatile organic compounds through gas chromatography retention time and ion mobility time; and screening symbolic differences from the measured volatile organic compounds by adopting a partial least squares discriminant analysis method, and carrying out semi-quantitative comparative analysis, thereby carrying out rapid and accurate difference analysis on the quality of the thin-skinned melon fruits after application of forchlorfenuron. The method is advantaged in that gas chromatography (GC) and ion mobility spectrometry (IMS) are combined, the outstanding separation characteristic of GC and advantages of quick response and high sensitivity of IMS are utilized, andthe identification method further has properties of low detection cost, high sensitivity, simple and convenient process, simple sample pretreatment, high analysis speed and visual result.
Owner:INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS

Triazophos monoclonal antibody and application thereof to colloidal gold detection test paper

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.
Owner:SUZHOU KUAIJIEKANG BIOTECH CO LTD
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