The invention discloses a high-stability zero-rigidity micro-vibration isolation device based on a magneto-rheological technology. The vibration isolation device comprises a horizontal vibration isolation supporting frame and a vertical vibration isolation mechanism, wherein the vertical vibration isolation mechanism is fixed on a horizontal vibration isolation table of the horizontal vibration isolation support frame, a workbench is arranged over the vertical vibration isolation mechanism and is connected with the vertical vibration isolation mechanism through a screw rod, and magneto-rheological fluid is poured into the positions below the horizontal vibration isolation table of the horizontal vibration isolation platform, in the center of the vertical vibration isolation mechanism, andin a center circular ring of the vertical vibration isolation structure below the workbench, so that liquid damping is changed by changing the current intensity. According to the vibration isolation device, an ultra-low horizontal and vertical direction inherent frequency is achieved, a good vibration isolation effect is achieved, the contradiction between high bearing capability and low inherentfrequency is well solved, the whole mechanism has the characteristics of high stability and zero rigidity, the extremely low vibration transmission rate is achieved, and the vibration isolation devicehas the advantages of being small in size, simple and compact in structure, stable in performance and the like.