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Chlopyrifos monoclonal antibody and preparation method and application thereof

A monoclonal antibody, chlorpyrifos technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as rapid detection of difficult and complex matrix samples, and achieve the effect of being easy to popularize, easy to popularize and apply, and to determine the image of results

Active Publication Date: 2019-06-28
SUZHOU KUAIJIEKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity of the reported chlorpyrifos colloidal gold test strips is usually difficult to achieve rapid detection of complex matrix samples

Method used

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  • Chlopyrifos monoclonal antibody and preparation method and application thereof
  • Chlopyrifos monoclonal antibody and preparation method and application thereof
  • Chlopyrifos monoclonal antibody and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Embodiment: a preparation method of chlorpyrifos monoclonal antibody, comprising the following steps:

[0038] 1. Synthesis of Chlorpyrifos Hapten

[0039] Synthesis of O-ethyl-O-(3,5,6-tripyridyl)phosphorylthiochloride. Weigh 4.26 g of 3,5,6-trichloro-2-hydroxypyridine and dissolve it in acetonitrile. At room temperature, the solution was added dropwise to 3.83 g of O-ethylphosphorylthiochloride dissolved in 20 mL of acetonitrile, powdered K 2 CO 3 8.7g of the mixed solution was reacted for 1h. After the reaction was completed, it was vacuum filtered and the solvent was evaporated to obtain a yellow oil. After column chromatography, washing with petroleum ether / dichloromethane (8:1, volume ratio), the solvent was evaporated to obtain 6.35 g of light yellow oily liquid (87% yield).

[0040] Synthesis of O-ethyl-O-(3,5,6 trichloropyridyl)-N-(butyric acid) phosphorothioate. Weigh 0.54g of 4-aminobutyric acid and 0.78g of KOH, dissolve them in 6mL of methanol, cool i...

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Abstract

The invention relates to a chlopyrifos monoclonal antibody and a preparation method and application thereof. The chlopyrifos monoclonal antibody and preparation method and application thereof have theadvantages that the specificity and sensitivity are high, the method is simple and rapid, and a result is displayed vividly, visually and accurately; the cost is reduced, the application range is wide, convenience is provided for popularization; and via the prepared chlopyrifos colloidal gold test paper, the result can be determined within minutes according to strips presented after reaction needless of professionals or auxiliary instruments, operation is simple and rapid, the result is visual and accurate, the cost is low, and detection can be carried out in the outside.

Description

technical field [0001] The invention relates to the field of agricultural product detection, in particular to a chlorpyrifos monoclonal antibody. Background technique [0002] Chlorpyrifos (Chlorpyrifos), also known as chlorpyrifos, belongs to phosphorothioate insecticides. It destroys the normal nerve impulse conduction by inhibiting the activity of acetylcholinesterase AChE or cholinesterase ChE, causing a series of poisoning symptoms: Excitement, convulsions, paralysis, death. Mainly used to control rice planthoppers, rice leaf rollers, cotton aphids, Spodoptera litura, cabbage caterpillars, diamondback moths, aphids, root maggots and other agricultural pests. [0003] At present, the detection methods for pesticide residues are mainly instrumental analysis methods, including high performance liquid chromatography (HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), Liquid-mass tandem method (HPLC-MS) and gas-m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/559G01N33/553G01N33/577
Inventor 盛相国
Owner SUZHOU KUAIJIEKANG BIOTECH CO LTD
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