Cylinder plate method-based leather material bacteriostasis effect test method

A detection method, the cup and saucer method, is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of lack of antibacterial effect detection methods and less research on antibacterial performance, so as to achieve difficult observation and recording, Simple operation and accurate results

Inactive Publication Date: 2012-07-18
中华人民共和国吴江出入境检验检疫局
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the antibacterial properties of leather products, and there is a lack of better antibacterial effect detection methods

Method used

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  • Cylinder plate method-based leather material bacteriostasis effect test method
  • Cylinder plate method-based leather material bacteriostasis effect test method
  • Cylinder plate method-based leather material bacteriostasis effect test method

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Experimental program
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Embodiment Construction

[0034] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0035] 1. Materials

[0036] 1.1 Sample: Leather from Fuhua Leather Co., Ltd., Quzhou, Zhejiang (anti-mold treatment, no anti-mold treatment)

[0037] 1.2 Culture medium: beef extract peptone solid medium, beef extract peptone semi-solid medium, beef extract peptone liquid medium

[0038] 1.3 strains

[0039] Escherichia coli 1.358; Staphylococcus aureus 1.801; Bacillus subtilis 1.108.

[0040] 1.4 Drugs and reagents

[0041] 2,3,5-Triphenyltetrazolium chloride (TTC), NaCl, etc. are domestic analytical grade

[0042] 1.5 Vessels and Instruments

[0043] Petri dishes, test tubes, Erlenmeyer flasks, pipettes, pipette guns, suction balls, inoculation loops;

[0044] Bacteria counting plate; electronic analytical balance; vacuum drying oven; binocular microscope; biochemical incubator; ultra-clean workbench; vertical pressure steam...

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Abstract

The invention discloses a cylinder plate method-based leather material bacteriostasis effect test method. The test method includes the following steps: (1) sample preparation; (2) 2,3,5-triphenyltetrazolium chloride solid medium preparation; (3) 2,3,5-triphenyltetrazolium chloride semisolid medium preparation; (4) inoculant suspension preparation; (5) testing operation; (6) evaluation and reporting. The cylinder plate method-based leather material bacteriostasis effect test method solves the defects of small bacterial colony, nontransparent sample and uneasy observation and recording; the test method is easy to operate, the result is accurate, the repeatability is good, and the test method has a high application value.

Description

technical field [0001] The invention relates to a test method, in particular to a test method for antibacterial effect of leather materials based on the cup and saucer method. Background technique [0002] There are a large number of invisible microorganisms in the human living environment, including various bacteria and fungi. Under normal circumstances, some resident bacteria on people's skin play a role in protecting the skin from pathogenic bacteria, but once the body's immunity is weakened, the exfoliated dander, sweat secreted and suitable temperature and humidity can also protect the skin. Provide a good environment for the reproduction of bacteria, and a small amount of pathogenic bacteria in them will multiply in large numbers, causing the imbalance of the ecological balance of the flora on the skin, and invading the skin to cause diseases. [0003] Leather products, as the most consumed leather products, are more conducive to the growth of fungi due to sweat and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
Inventor 任信朱小卫朱建华
Owner 中华人民共和国吴江出入境检验检疫局
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