Primers, probes, detection system and kit for one time detection of intestinal cancer multiple gene mutation

Abstract

The present invention discloses primers, probes, a detection system and a kit for one time detection of intestinal cancer multiple gene mutation, wherein the primers, the probes, and the distribution way for detecting gene mutations of intestinal cancer genes such as KRAS, NRAS, PIK3CA, and BRAF are provided. According to the present invention, the detection kit adopts the 12 linking PCR reaction strip design, each 12 linking PCR strip detects multiple genes of a sample, the corresponding detection reagents for the 37 mutations of KRAS / NRAS / PIK3CA / BRAF and the internal control reagents are filled in the pipes 1-11 of the 12 linking PCR strip, the mutation is indicated by the FAM signal, and the internal control is indicated by the HEX (or VIC) signal; the pipe 12 is adopted as the DNA extraction quality external control detection pipe and is indicated by the FAM; and with the primers, the probes, the detection system and the kit, the one-time detection of the 37 mutations of the KRAS / NRAS / PIK3CA / BRAF gene can be achieved, such that the detection time is substantially shortened, the sensitivity is high, the specificity is strong, the operation is simple and rapid, and the reference for selection of tumor targeting drug therapy on intestinal cancer patients can be provided for clinician.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to primers, probes, detection kits and systems for lung cancer multiple gene mutations. Background technique [0002] Colorectal cancer is the third most common malignant tumor in the world, and its mortality rate is the second highest, with about 1.2 million new patients every year. In China, among all malignant tumors, colorectal cancer has become the third most fatal malignant tumor. The treatment of colorectal cancer is an active comprehensive treatment based on surgery, which can remove the primary tumor and relieve the obstructive symptoms caused by the tumor. Postoperative chemotherapy has developed rapidly in recent years, supplemented by immunotherapy, traditional Chinese medicine therapy, gene-targeted therapy, especially targeted therapy is increasingly used in clinical practice. The occurrence of colorectal cancer is multi-stage, multi-step, involving complex processes suc...

AI Technical Summary

Problems solved by technology

Therefore, it is very important to detect the genetic variation of KRAS / NRAS / PIK3CA / BRAF gene in colorectal cancer. However, most of the current clinical practice can only detect one mutation or a mutation of a gene at a time, and the detection throughput is low and time-consuming. A kit capable of simultaneously and one-time detection of the four gene mutations

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