Application of AhR inhibitor to induction of chondrogenic differentiation of mesenchymal stem cells and treatment of related diseases

A mesenchymal stem cell and inhibitor technology, applied in the field of stem cell therapy, can solve the problems that there are no reports on the proliferation and cartilage differentiation of umbilical cord mesenchymal stem cells, and achieve the effect of overcoming the weak proliferation and differentiation ability

Inactive Publication Date: 2022-03-29
宁夏厚泽生物医药科技有限公司
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AI Technical Summary

Problems solved by technology

[0004] Ginseng epoxy acetylenic alcohol is a natural organic compound with various activities, but there is no report on the effect on AhR and the regulation of umbilical cord mesenchymal stem cell proliferation and cartilage differentiation

Method used

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  • Application of AhR inhibitor to induction of chondrogenic differentiation of mesenchymal stem cells and treatment of related diseases
  • Application of AhR inhibitor to induction of chondrogenic differentiation of mesenchymal stem cells and treatment of related diseases
  • Application of AhR inhibitor to induction of chondrogenic differentiation of mesenchymal stem cells and treatment of related diseases

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Embodiment Construction

[0017] 1. Materials

[0018] Human umbilical cord mesenchymal stem cells and chondrogenic differentiation medium of umbilical cord mesenchymal stem cells were purchased from Saiye (Guangzhou) Biotechnology Co., Ltd. DMEM / F12 culture medium and fetal bovine serum were purchased from Gibco, USA. CCK-8 detection reagents, double antibodies, and BCA protein concentration detection kits were purchased from Shanghai Biyuntian Biotechnology. Ginseng epoxy acetylenic alcohol was purchased from Yuanye Biology, with a purity of ≥95%.

[0019] 2. Method

[0020] 1. Recovery and culture of umbilical cord mesenchymal stem cells

[0021] Take out the human umbilical cord mesenchymal stem cell cryopreservation tube, immediately put it into a constant temperature water bath at 37°C, wash it with DMEM / F12 culture medium after thawing and dissolving, fully pipette, and centrifuge at 1000rpm for 10min at room temperature, discard the supernatant, and use 10% fetus Cells were resuspended in D...

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Abstract

The invention discloses application of an AhR inhibitor in inducing chondrogenic differentiation of mesenchymal stem cells and treating related diseases. The invention finds that ginseng epoxy alkynol can promote proliferation and cartilage differentiation of umbilical cord mesenchymal stem cells by inhibiting AhR; therefore, the application of the culture medium to the in-vitro induced differentiation culture of the umbilical cord mesenchymal stem cells is helpful for overcoming the defects of weak proliferation capacity and differentiation capacity when the umbilical cord mesenchymal stem cells are induced to be differentiated into cartilage cells by a conventional method.

Description

technical field [0001] The invention belongs to the field of stem cell therapy, and specifically relates to the use of an AhR inhibitor to induce chondrogenic differentiation of mesenchymal stem cells and treat related diseases. Background technique [0002] Articular cartilage is mainly composed of chondrocytes and extracellular matrix, without blood vessels and nervous system, and chondrocytes have extremely weak proliferation ability and lack of regenerative ability, which makes it difficult for cartilage to repair itself after injury. The current clinical treatment methods for articular cartilage injury include bone marrow stimulation repair and chondrocyte transplantation repair, but the cartilage repair effect obtained is not satisfactory. With the development of science and technology, related therapies based on stem cells provide a new therapeutic direction for the treatment of cartilage injuries. Umbilical cord mesenchymal stem cells can be induced to differentiate...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K31/336A61P19/08
CPCC12N5/0668A61K31/336A61P19/08C12N2500/30
Inventor 不公告发明人
Owner 宁夏厚泽生物医药科技有限公司
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