A thermosensitive collagen-based hydrogel loaded with bioactive polypeptide and its preparation method

Abstract

The invention discloses a temperature-sensitive collagen-based hydrogel loaded with biologically active polypeptides for repairing osteoarticular cartilage defects and a preparation method thereof. The thermosensitive collagen-based hydrogel is made of N-vinylcaprolactam, methacrylic acid, recombinant human type III collagen, bioactive peptides and PLGA, etc. It has good biocompatibility, strong cell attachment ability, It is non-toxic, degradable and non-immunogenic, and has a good repairing effect on animal cartilage defects. It utilizes the injectability of thermosensitive collagen-based hydrogel, the elasticity-enhancing potential of type III collagen and the potential of promoting cartilage differentiation, combined with the promotion effect of bioactive peptides on the repair of articular cartilage damage, making it a new product that meets the current needs. It is a tissue regeneration material that is excellently suitable for the repair and treatment of clinical cartilage defects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a thermosensitive collagen-based hydrogel loaded with biologically active polypeptides for repairing osteoarticular cartilage defects and a preparation method thereof. Background technique [0002] The self-repair ability of articular cartilage is low. If long-term lack of treatment, the condition of the injured part will gradually deteriorate, and eventually it may develop into knee osteoarthritis (KOA), which seriously affects the quality of life of patients. Nowadays, the traditional therapies for the treatment of cartilage defects mainly include: articular cartilage grinding and plastic surgery, joint debridement, subchondral bone drilling, microfracture, autologous osteochondral transplantation, etc. Although these traditional therapies can to a certain extent Repairing damaged cartilage requires surgery, which is often intimidating to many patients. Therefore, a ther...

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Problems solved by technology

However, this patent does not involve research on cytotoxicity, nor does it carry out research on the repair of cartilage tissue, and the effects of different subtypes of recombinant collagen have not been carried out in depth

Chinese patent CN 103751102A discloses a collagenase temperature-sensitive hydrogel and its preparation method and application, which contains hydrogel and collagenase polysaccharide particles. However, this patent is used for the treatment of palmar aponeurosis contracture and does not involve the articular cartilage Repair, and its in vitro release time is only 7 days

Chinese patent CN 105664250A discloses an injectable and degradable temperature-sensitive hydrogel and its preparation method. By adding a variety of natural polymers (sodium alginate, chitosan, collagen and hyaluronic acid) to make the hydrogel The hydrogel is biodegradable, and the main body of the hydrogel is poly-N-isopropylacrylamide, but studies by Zhang Wenjing and others have shown (Zhang Wenjing, Mao Zhengwei, Gao Changyou. Preparation and characterization of PNIPAM microgel particles and their interaction with cells Interaction. Proceedings of the Ninth National Symposium on Polymer Molecular and Structural Characterization. Hangzhou, 2014: 343-344): Precipitation polymerization of NIPAM, polyethylene glycol diacrylate and acrylic acid Swellability of cross-linked polyisopropylacrylamide (PNIPAM) microgel particles, the microgel particles have some cytotoxicity to cells at 25 °C, and the patent does not conduct cytotoxicity studies on hybrid hydrogels , without mentioning specific areas of tissue repair

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