Preparation for mobilizing mesenchymal stem cells and method for separating mesenchymal stem cells

A technology of mesenchymal stem cells and preparations, applied in the field of preparation and separation of mobilized mesenchymal stem cells, can solve the problems of limited cell sources, cumbersome culture steps, pollution, etc., and achieve the effect of high mobilization efficiency
CN103146646AInactive Publication Date: 2013-06-12ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2013-06-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a preparation for mobilizing mesenchymal stem cells and a method for separating the mesenchymal stem cells. The preparation for mobilizing the mesenchymal stem cells comprises CoCl2, wherein the CoCl2 is a hypoxia mimetic agent, and the dosage is in a range of 5-20mg / kg. The CoCl2 and AMD3100 are used in a combined manner. The dosage of the AMD3100 is 5mg / kg. According to the method for separating the mesenchymal stem cells by using the preparation, the preparation is used for actuating the mesenchymal stem cells to be mobilized, enter peripheral blood and then be separated. The method comprises the following separation steps of: sampling the periphery blood, separating mononuclear cells by using a lymphocyte separating medium, performing resuspension by using a DMEM (dulbeccos modified eagle medium) containing 20% of fetal bovine serum, inoculating to a culture flask, culturing for 7 days, and changing a culture solution, thus obtaining the mesenchymal stem cells of the peripheral blood on the 10th day. The mesenchymal stem cells of the peripheral blood highly express CD90, CD29 and CD44, do not express CD45 and CD34, and have the capacities of in vitro bone formation, fat formation and cartilage differentiation formation. The preparation is high in MSCs (mesenchymal stem cells) mobilization efficiency, and an effective preparation and an effective method are provided for tissue engineering and regenerative medicine.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and mainly relates to a preparation and separation method for mobilizing mesenchymal stem cells. Background technique

[0002] Mesenchymal stem cells (MSCs) are adult stem cells with strong proliferation ability and multilineage differentiation potential. Since MSCs can differentiate into bone, cartilage, fat, muscle, tendon, nerve and other tissues under certain conditions, MSCs have become very potential seed cells for tissue engineering and regenerative medicine. MSCs have broad clinical application prospects: treating bone, joint, and tendon defects and injuries; promoting regeneration of nerve cells, cardiomyocytes, and liver cells; supporting hematopoiesis and promoting hematopoietic stem cell implantation; preventing and treating graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation ); Immunomodulatory treatment of autoimmune disorders, etc.

[0003] Bone marrow is t...

Claims

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