Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel use of dimethyloxalglycine and mesenchymal stem cell separation method

A dimethyloxalylglycine, stem cell technology, applied in animal cells, extracellular fluid diseases, vertebrate cells, etc., can solve the problems of iron chelator loss, lack of MSCs mobilization agent, and inability to become Effects of Cell Contamination

Active Publication Date: 2013-11-27
ZHEJIANG CHINESE MEDICAL UNIVERSITY
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Members of our research group have found that the Fe2+ competitor CoCl2 can mobilize MSCs. However, iron chelators themselves have side effects such as iron loss and cannot be ideal mobilizers for MSCs.
Therefore, there is still a lack of safer and more efficient MSCs mobilization agents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel use of dimethyloxalglycine and mesenchymal stem cell separation method
  • Novel use of dimethyloxalglycine and mesenchymal stem cell separation method
  • Novel use of dimethyloxalglycine and mesenchymal stem cell separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] The present invention will be further described below in conjunction with specific examples. It should be understood that the following examples are only used to illustrate the present invention and are not intended to limit the protection scope of the present invention.

[0040] The methods used in the following examples are conventional methods unless otherwise specified. The materials or reagents required in the following examples are commercially available unless otherwise specified. The ways of obtaining various biological materials described in the examples are only experimental ways to achieve the purpose of specific disclosure, and should not be a limitation on the sources of biological materials in the present invention.

[0041] Unless otherwise specified, the percentage concentrations are mass / volume (W / V) percentage concentrations or volume / volume (V / V) percentage concentrations.

[0042] The specific MSCs mobilization techniques are as follows:

[0043] 1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biology and mainly relates to novel use of dimethyloxalglycine and a mesenchymal stem cell separation method. The invention aims to provide the dimethyloxalglycine; the dimethyloxalglycine is used for preparing medicines expressed by up-regulated myeloid tissue hypoxia-inducible factor-1, used for preparing medicines for increasing quantity of mesenchymal stem cells, used for preparing medicines for mobilizing mesenchymal stem cells, and used for preparing mesenchymal stem cell medicines. The invention further provides a mesenchymal stem cell separation method by adopting the dimethyloxalglycine. The mesenchymal stem cell separation method is simpler and high-efficient, the peripheral blood mesenchymal stem cell obtained has capacity of osteogenesis in vitro and adipogenic differentiation, and cell pollution can be avoided preferably.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to a new application of dimethyloxalylglycine and a method for separating mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are adult stem cells with strong proliferative ability and multi-directional differentiation potential, which can be used to treat bone, joint, tendon and other tissue injuries; promote the regeneration of nerve cells, cardiomyocytes, liver cells, etc. . At the same time, MSCs can support hematopoiesis, regulate immune function, and prevent graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation, and have broad clinical application prospects. [0003] MSCs mobilization (mobilization) refers to the process of MSCs leaving the bone marrow microenvironment and entering the peripheral circulation. Under physiological conditions, the content of MSCs in the peripheral circulation of adults is very small. St...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/198A61P19/08A61P19/02A61P19/04A61P25/00A61P9/00A61P1/16A61P7/06A61P37/02A61P37/06C12N5/0775C12N5/0789
Inventor 余勤刘丽珍刘伟
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com