Mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles as well as preparation method and application thereof

A nanoparticle and drug-loaded nanotechnology, which is applied in nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of localized damage and inability to guarantee the effective function of drugs.

Active Publication Date: 2020-08-28
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cartilage defect is not the same as extensive cartilage lesions in osteoarthritis. Its main damaged part is relatively limited. Even if the therapeutic drug is injected into the joint cavity, it cannot guarantee that the drug can effectively play a role in the damaged part. Therefore, how to make the drug stable and efficient Transport to the lesion site is the key to effective drug efficacy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles as well as preparation method and application thereof
  • Mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles as well as preparation method and application thereof
  • Mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] The present invention provides a method for preparing mesenchymal stem cell membrane-camouflaged magnetic drug-loaded nanoparticles described in the above technical solution, comprising the following steps:

[0044] The drug-containing solution and Fe 3 o 4 The nanoparticles were co-incubated at 20-30°C for 22-26 hours to obtain the drug-loaded Fe 3 o 4 Nanoparticle dispersion; the drug is selected from drugs that can promote cartilage differentiation and repair;

[0045] Mesenchymal stem cell membrane (BMSC) and drug-loaded Fe 3 o 4 The nanoparticle dispersion liquid is mixed and then incubated with ultrasound for coating, so as to obtain mesenchymal stem cell membrane-disguised magnetic drug-loaded nanoparticles.

[0046] The present invention will contain the solution of medicine and Fe 3 o 4 The nanoparticles were co-incubated at 20-30°C for 22-26 hours to obtain the drug-loaded Fe 3 o 4 nanoparticle dispersion. In the present invention, the co-incubation ...

Embodiment 1

[0067] Preparation of drug-loaded nanoparticles: Take 1.08g FeCl 3 ·6H 2 O, 0.24g NaCit 2H 2 O, 1.20g NaAc was dissolved in 20ml ethylene glycol, ultrasonically dissolved and magnetically stirred for 0.5h. Transfer to a hydrothermal kettle and react at 200° C. for 10 h. After the reaction, rinse the reactor with cold water to room temperature. The product was washed 3 times with water and 3 times with alcohol, and placed in ethanol for later use. Weigh 3mg KGN into a 5ml EP tube, add 100μL DMSO to dissolve, weigh 15mg Fe 3 o 4 Nanoparticles, mix the two into a 15mL centrifuge tube, make up to 15mL with double distilled water, put it in a rotary mixer and rotate for 24h, and obtain the drug-loaded Fe 3 o 4 nanoparticles;

[0068]Membrane extraction: Stem cells cultured in a culture dish to a confluence of 70%-80% are washed with 1×PBS, digested with 0.05% trypsin-EDTA, terminated, centrifuged at 1000rpm for 10min, and the supernatant is discarded. Add 0.25mmol / L EDTA·2N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
depthaaaaaaaaaa
Login to view more

Abstract

The invention provides mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles as well as a preparation method and application thereof. The drug-loaded nanoparticles comprise drug-loaded Fe3O4 nanoparticles and a mesenchymal stem cell membrane compounded on the surfaces of the drug-loaded Fe3O4 nanoparticles, wherein the mass ratio of a drug to Fe3O4 nanoparticles in the drug-loaded Fe3O4 nanoparticles is 1:(4.5-5.5); the drug is selected from drugs capable of promoting cartilage differentiation and repair; and the ratio of the mass of the drug-loaded Fe3O4 nanoparticlesto the number of cell sources of the mesenchymal stem cell membrane is (3.4-3.9) mg: 5x10<6>. According to the drug-loaded nanoparticles, the mesenchymal stem cell membrane is taken as a carrier, andthe Fe3O4 nanoparticles loaded with the drug capable of promoting cartilage differentiation and repair are enveloped, so that the drug can be stably and efficiently transported to a diseased region, and the repair of cartilage tissues can be promoted. The mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles are non-toxic, degradable and good in biocompatibility; and the effect of promoting cartilage repair is superior to that of pure local medication.

Description

technical field [0001] The invention belongs to the technical field of cell membrane-disguised nanoparticles, and in particular relates to a mesenchymal stem cell membrane-disguised magnetic drug-loaded nanoparticle and a preparation method and application thereof. Background technique [0002] Articular cartilage defects caused by trauma or overuse may cause joint pain, affect joint mobility, and advance the progression of osteoarthritis. At present, the treatment of cartilage defects has limitations, and the main treatment methods are limited to microfracture and cartilage transplantation. However, for larger defects, microfracture treatment may not play a big role, and it is difficult for patients to recover quickly. Therefore, , most doctors will choose cartilage transplantation to repair larger defects. [0003] The advantage of cartilage transplantation is that the operation is simple and the risk is low. The main component of transplanted cartilage is hyaline cartila...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/46A61K31/192A61P19/04B82Y5/00
CPCA61K9/5115A61K9/5184A61K31/192A61P19/04B82Y5/00
Inventor 陈俊陈世益张兴宇李云霞
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap