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Injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and preparation method thereof

A cartilage repair and osteochondral technology, which is applied in the fields of medical formula, medical science, drug delivery, etc., can solve problems such as difficult to meet the distribution of cartilage defect tissue morphology, fast release of growth factors or drugs, and unsatisfactory therapeutic effects, etc., to achieve Long-term drug release, good interface connectivity, and the effect of satisfying the long-term repair process

Pending Publication Date: 2020-12-22
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the rapid degradation of hydrogel materials, the loaded growth factors or drugs are released too quickly, which makes the therapeutic effect unsatisfactory, and most of the existing scaffold materials are used for cartilage repair after in vitro molding, which is difficult to meet the needs of cartilage defects. Irregular morphological distribution of tissues, and clinical operation is also difficult

Method used

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  • Injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and preparation method thereof
  • Injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and preparation method thereof
  • Injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and preparation method thereof

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Effect test

Embodiment 1

[0031] A method for preparing an injectable double-layer drug-loaded osteochondral repair hydrogel scaffold, comprising the following steps:

[0032] (1) Prepare the bone repair layer gel precursor, prepare a PBS solution containing LAP (phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt), mix KGN (kartogenin) with acryloyl ring Dextrin was dissolved in the above PBS solution in equimolar ratio, methacrylylated gelatin and methacrylylated sodium alginate were uniformly dissolved in the above PBS solution, and α-TCP (α-tricalcium phosphate) was added to disperse evenly , add rat bone marrow mesenchymal stem cells and mix evenly and store in the dark for later use. The mass fraction of LAP in the obtained precursor solution is 0.05%, the concentration of KGN and acryloyl cyclodextrin is 1uM, and the concentration of methacrylylated seaweed The mass fraction of sodium nitrate is 2%, the mass fraction of methacryloyl gelatin is 3%, the mass fraction of α-TCP is 0.5%, and the c...

Embodiment 2

[0038] A method for preparing an injectable double-layer drug-loaded osteochondral repair hydrogel scaffold, comprising the following steps:

[0039] (1) Prepare the bone repair layer gel precursor, prepare PBS containing LAP, dissolve KGN and acryloyl cyclodextrin in the above PBS solution in an equimolar ratio, and mix methacrylylated hyaluronic acid with formazan Acrylylated silk fibroin was uniformly dissolved in the above solution, hydroxyapatite was added to disperse evenly, and rat bone marrow mesenchymal stem cells were added to mix evenly and then stored in the dark for later use. The mass fraction of LAP in the obtained precursor solution was 0.01 %, the concentration of KGN and acryloyl cyclodextrin is 1uM, the mass fraction of methacrylylated hyaluronic acid is 4%, the mass fraction of methacrylated silk fibroin is 1%, and the mass fraction of hydroxyapatite 1%, the concentration of rat bone marrow mesenchymal stem cells is 10 8 / ml.

[0040] (2) Prepare the gel ...

Embodiment 3

[0044] A method for preparing an injectable double-layer drug-loaded osteochondral repair hydrogel scaffold, comprising the following steps:

[0045] (1) Prepare the bone repair layer gel precursor, prepare a PBS solution containing Irgacure2959 (2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone), and mix KGN and acryloyl ring Dextrin was dissolved in the above PBS solution at an equimolar ratio, methacrylated chitosan and methacrylated collagen were uniformly dissolved in the above PBS solution, and β-TCP (β-tricalcium phosphate) was added to disperse Uniformly, add rat bone marrow mesenchymal stem cells and mix evenly and store in the dark for later use. In the obtained precursor solution, the mass fraction of Irgacure2959 is 0.5%, the molar concentration of KGN and acryloyl cyclodextrin is 1uM, methyl The mass fraction of acrylylated chitosan is 3%, the mass fraction of methacrylated collagen is 2%, the mass fraction of β-TCP is 2%, and the concentration of rat bone marr...

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Abstract

The invention relates to an injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and a preparation method thereof, and belongs to the field of biomedical materials. The double-layer drug-loaded osteochondral repair hydrogel scaffold comprises a methylacryloyl polysaccharide and protein natural polymer material, an ultraviolet initiator, acryloyl chloride modified cyclodextrin, a bone or cartilage differentiation promoting drug, a metal ion source and seed cells. The osteochondral integrated repair scaffold prepared by the method has the advantages of good biocompatibility, easy degradation, easy shaping and the like; the raw material source is wide, and the double-layer scaffold is connected by chemical bonds to form the integrated scaffold, so that the problem of infirm bonding of an interface layer is avoided; and the bone or cartilage differentiation promoting drug is loaded on the bone-cartilage repair scaffold material, osteogenic differentiation is induced under the adjustment of metal ions contained in a bone repair layer, and cartilage differentiation is induced in a cartilage repair layer, so that the differentiation of the seed cells to target tissues is induced, and the scaffold can be applied to osteochondral tissue repair.

Description

technical field [0001] The invention relates to an injectable double-layer drug-loaded osteochondral repair hydrogel scaffold and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Articular cartilage is a highly differentiated connective tissue that absorbs stress, dampens shock, and provides a low-friction environment for joints. However, tumors, infections, trauma, and degenerative osteoarthritis can all cause defects in articular cartilage. limited. Osteochondral defect is caused by the extension of articular cartilage defect into the subchondral bone. If the subchondral bone is not completely repaired, it will be transformed into a change in the biomechanical properties of the entire osteochondral bone, thereby affecting the effect of cartilage repair. For the treatment of osteochondral defects, arthroscopic debridement and microfracture are palliative clinical treatments, while osteochondral transplantation and...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/44A61L27/46A61L27/52A61L27/54C08J3/28C08J3/075C08J3/24C08L89/00C08L5/04C08L5/08C08L1/10
CPCA61L27/3847A61L27/3852A61L27/446A61L27/46A61L27/52A61L27/54A61L2300/102A61L2300/412A61L2300/602A61L2400/06A61L2430/02A61L2430/06C08J3/075C08J3/246C08J3/28C08J2301/10C08J2305/08C08J2389/00C08J2405/04C08J2489/00
Inventor 戴红莲张向可庄彭真
Owner WUHAN UNIV OF TECH
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