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Dynamic imine bond polysaccharide polymer-based injectable hydrogel and preparation method and application thereof

An imine bond and polysaccharide technology, which is applied in the field of polysaccharide polymer injectable hydrogel and its preparation, can solve the problems of poor mechanical strength, inability to meet application requirements for repairing cartilage tissue, long gel time, and the like, Achieve the effect of strong biocompatibility, good biocompatibility and biodegradability, and high repetition rate

Inactive Publication Date: 2019-05-07
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the gels formed by using natural sodium alginate and chitosan often have problems such as long gel time, poor mechanical strength, and gelation in an acidic or alkaline environment, which cannot meet the application requirements for repairing cartilage tissue.

Method used

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  • Dynamic imine bond polysaccharide polymer-based injectable hydrogel and preparation method and application thereof
  • Dynamic imine bond polysaccharide polymer-based injectable hydrogel and preparation method and application thereof
  • Dynamic imine bond polysaccharide polymer-based injectable hydrogel and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Dissolve 10g of sodium alginate in 50mL of absolute ethanol with an analytical balance, dissolve 12g of sodium periodate in 50mL of deionized water, and stir magnetically for 20 minutes. After the solution was uniformly dissolved, the sodium periodate solution was mixed into the sodium alginate solution, protected from light, and reacted at 25°C for 6 hours. After the reaction is complete, measure 20 mL of ethylene glycol into the reaction system with a pipette gun, and stop the reaction after magnetically stirring for 2 h. Purified oxidized sodium alginate was obtained after suction filtration twice, dialysis for 3 days, and freeze-drying for 3 days.

[0048]2. Weigh 2.04g of dimethyl 3,3'-dithiodipropionate in 50mL of anhydrous methanol with an analytical balance, add 2.55ml of hydrazine hydrate, stir magnetically at 10°C for 14 hours, and filter under reduced pressure , The resulting product was washed and filtered with 20ml of methanol, 4ml of water, and 20ml of...

Embodiment 2

[0053] 1. Dissolve 10g of sodium alginate in 50mL of absolute ethanol with an analytical balance, dissolve 6.12g of sodium periodate in 30mL of deionized water, and stir magnetically for 20 minutes. After the solution was uniformly dissolved, the sodium periodate solution was mixed into the sodium alginate solution, protected from light, and reacted at 25°C for 5 hours. After the reaction is complete, measure 12 mL of ethylene glycol into the reaction system with a pipette gun, and stop the reaction after magnetic stirring for 1 h. Purified oxidized sodium alginate was obtained after suction filtration twice, dialysis for 3 days, and freeze-drying for 3 days.

[0054] 2. Weigh 1.02g dimethyl 3,3'-dithiodipropionate in 50mL of anhydrous methanol with an analytical balance, add 1.27g hydrazine hydrate, stir magnetically at room temperature at 25°C for 10 hours, pump under reduced pressure The obtained product was washed and filtered with 15ml of methanol, 3ml of water, and 10ml...

Embodiment 3

[0058] 1. Weigh 10g of sodium alginate and dissolve in 50mL of absolute ethanol with an analytical balance, weigh 7.8g of potassium permanganate and dissolve in 50mL of deionized water, and stir magnetically for 20 minutes. After the solution was uniformly dissolved, the sodium periodate solution was mixed into the sodium alginate solution, protected from light, and reacted at 15°C for 5 hours. After the reaction was complete, 3g of NaCl was added, purified with ethanol, filtered twice with suction, dialyzed for 3 days, and freeze-dried for 3 days to obtain purified oxidized sodium alginate.

[0059] 2. Dissolve 1.95ml of dimethyl 3,3'-dithiodipropionate in 50mL of anhydrous methanol with a pipette gun, add 2.5ml of hydrazine hydrate, stir magnetically at room temperature overnight, and filter under reduced pressure , The resulting product was washed and filtered with 20ml of methanol, 4ml of water, and 10ml of methanol in turn, and dried. The white powder was the product.

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Abstract

The present invention relates to a dynamic imine bond polysaccharide polymer-based injectable hydrogel and a preparation method and an application thereof. The dynamic imine bond polysaccharide polymer-based injectable hydrogel comprises the following raw material components in weight percentages: 5-18% of a component A: oxidized sodium alginate, 0.1-20% of a component B: a mixture of one or two of water-soluble chitosan and 3,3'-dithiobis(propanohydrazide), and the balanced solvent. The materials are prepared into an even solution according to the ratio. Compared with the prior art, "Schiff base", amide bonds and other dynamic imine bonds are used to realize cross-linking and can form a gel under mild conditions of human body temperature and acid and alkali, and no biotoxic initiators areneeded. The hydrogel is fast in gel-forming speed and injectable after forming. The hydrogel is mild in the preparation conditions, and excellent in compression properties, biocompatibility and injectability, and has broad application prospects in the field of cartilage repair.

Description

technical field [0001] The invention relates to the technical field of injectable hydrogel, in particular to a dynamic imine bond-based polysaccharide polymer injectable hydrogel and its preparation method and application. Background technique [0002] Cartilage is a kind of tissue with extremely poor regenerative ability. Since it does not contain blood vessels and lymph, chondrocytes are difficult to migrate, and the tissue self-repair ability is poor. The repair after damage or loss is one of the major problems in clinical medicine. The current treatment methods are: Mainly cartilage transplantation. [0003] Hydrogel is a unique material with a three-dimensional network formed by the interaction between hydrophilic groups of polymers. A large amount of water is wrapped in the hydrogel material network, which is very similar to the structure of natural human tissue. It has attracted extensive attention in the field of tissue repair, such as implantation fillers in tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/52A61L27/58A61L24/08A61L24/00
Inventor 陈玉洁张润婧张楠汤隽劼李华刘河洲
Owner SHANGHAI JIAO TONG UNIV
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