Preparation method and application of cartilage-inducing matrix material

A matrix material, inductive technology, applied in medical science, prosthesis and other directions, can solve the problems of loss of biological characteristics, loss of tissue inductive ability, changes in physical and chemical properties of collagen products, etc., to achieve the effect of repair and regeneration

Active Publication Date: 2019-05-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Irradiation sterilization will not only lead to the breakage of some collagen molecular chains and loss of structure, but also may produce new cross-links and change its microstructure, which will eventually lead to changes in physical and chemical properties of collagen products and loss of biological properties, and loss of tissue induction ability
Moreover, the preparation of collagen sponges by freeze-drying and then irradiation sterilization will make it difficult to redissolve or gel the prepared collagen sponges, and cannot be used to prepare collagen-based biomedical materials with chondrogenic ability.

Method used

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  • Preparation method and application of cartilage-inducing matrix material
  • Preparation method and application of cartilage-inducing matrix material
  • Preparation method and application of cartilage-inducing matrix material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of a kind of cartilage inductive matrix material

[0044] In S1, the calfskin is subjected to depilation, segmentation, degreasing and heavy metal pretreatment. The specific operations are as follows:

[0045] A hair removal: use mechanical methods to scrape hair and skin and other debris and then fully clean;

[0046] B division: the depilated calfskin is cut into 1mm by cutting and pulverizing machine 3 small pieces;

[0047] C Degreasing: Add the cut calfskin to the degreasing solution of chloroform and ethanol with a volume ratio of 1:1 to degrease, the weight ratio of the degreasing solution to the cut calfskin is 6:1, and place it on a shaker at 4°C After shaking for 12 hours, replace the degreasing solution; after repeating the degreasing 4 times, add the degreased calfskin to clean in absolute ethanol, the weight ratio of the degreasing ethanol to the degreased calfskin is 10:1, and shake at 4°C After shaking on the bed for 12 ho...

Embodiment 2

[0058] Embodiment 2, the preparation of a kind of cartilage inductive matrix material

[0059] In S1, the calfskin is subjected to depilation, segmentation, degreasing and heavy metal pretreatment. The specific operations are as follows:

[0060] A hair removal: use mechanical methods to scrape hair and skin and other debris and then fully clean;

[0061] B division: the depilated calfskin is cut into 1mm by cutting and pulverizing machine 3 small pieces;

[0062] C Degreasing: Add the cut calfskin to the degreasing solution of chloroform and ethanol with a volume ratio of 1:1 to degrease, the weight ratio of the degreasing solution to the cut calfskin is 8:1, and place it on a shaker at 4°C After shaking for 10 hours, replace the degreasing solution; after repeating the degreasing 3 times, add the degreased calfskin to clean in absolute ethanol, the weight ratio of the degreasing ethanol to the degreased calfskin is 15:1, shake at 4°C After shaking on the bed for 10 hours,...

Embodiment 3

[0073] Embodiment 3, the preparation of a kind of cartilage inductive matrix material

[0074] In S1, the calfskin is subjected to depilation, segmentation, degreasing and heavy metal pretreatment. The specific operations are as follows:

[0075] A hair removal: use mechanical methods to scrape hair and skin and other debris and then fully clean;

[0076] B division: the depilated calfskin is cut into 1mm by cutting and pulverizing machine 3 small pieces;

[0077] C Degreasing: Add the cut calfskin to the degreasing solution of chloroform and ethanol with a volume ratio of 1:1, and the weight ratio of the degreasing solution to the cut calfskin is 10:1, and put it on a shaker at 4°C After shaking for 8 hours, replace the degreasing solution; after repeating degreasing 2 times, add the degreased calfskin to clean in absolute ethanol, the weight ratio of the degreasing ethanol to the degreased calfskin is 20:1, shake at 4°C After shaking on the bed for 8 hours, replace with a...

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Abstract

The invention belongs to the technical field of tissue-inducing biological material preparation, and particularly relates to a preparation method and application of a cartilage-inducing matrix material. The preparation method of the cartilage-inducing matrix material comprises the steps that calfskin is subjected to depilation, cutting, degreasing and heavy metal removal, and then sterilization, acid soaking and enzyme digestion treatment are carried out to obtain collagen liquid; finally, salting-out, purification, dialysis and neutralization are carried out on the collagen liquid to obtain the material. The matrix material prepared through the preparation method of the cartilage-inducing matrix material has the advantages of being high in bioactivity, good in biocompatibility and biodegradability and low in antigenicity, and the matrix material is used as a stem cell carrier and is induced for cartilage differentiation so that cartilage defects can be repaired and regenerated; the matrix material can also be used as a tissue engineering scaffold material, a soft tissue filling material and the like.

Description

technical field [0001] The invention belongs to the technical field of tissue-inductive biomaterial preparation, and in particular relates to a preparation method and application of a cartilage-inductive matrix material. Background technique [0002] Tissue-inducing material is a term for biomaterials proposed by Chinese scientists and recognized by the international community. The research direction and research content created by this has become a hot spot and focus in the field of international biomedical materials in the 21st century. Tissue inductive materials represented by osteoinductive artificial bone have been widely studied and achieved important results, and a large number of clinical practices have proved their good curative effect. The repair of cartilage defects is a long-standing clinical problem, and cartilage-inductive materials provide new ideas and approaches for the repair of cartilage defects. [0003] Based on the principles and methods of bionics, na...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/50A61L27/54A61L27/58
Inventor 林海郭立坤樊渝江张兴栋
Owner SICHUAN UNIV
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