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Implantable poly(1,8-octanediol-citrate) film modified through click chemistry and a preparing method thereof

A technology of polycitric acid and click chemistry, which is applied in medical science, coatings, prostheses, etc., can solve the problems of difficult application, expensive growth factors, and restrictions on in vitro research and transformation, and achieve the promotion of healing, excellent hydrophilicity, The effect of a wide range of raw material sources

Inactive Publication Date: 2017-11-17
THE UNIV OF HONG KONG SHENZHEN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, studies have shown that growth factor induction can effectively promote the differentiation of stem cells into tenocytes and promote the healing of damaged tendon tissue. However, growth factors are expensive, and most of them are only suitable for in vitro induction. Transformation of in vitro research to in vivo clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The sources of the actual medicines used in Example 1 and other examples are shown in Table 1 below.

[0031] Table 1 The source of some reagents and medicines used in the embodiments of the present invention

[0032] name Manufacturer Polydimethylsiloxane base Dow Corning Sylgard Hardener Dow Corning Sylgard Poly(1,8-octyl citrate) -- type I collagen GIBCO

[0033] The azide group-containing polyoctyl citrate 1,8-octane and the alkynyl-containing polyoctyl citrate 1,8-octyl citrate of the present invention are prepared by referring to published preparation procedures.

[0034] This example utilizes click chemistry to modify polyoctyl-1,8-octyl citrate to prepare a thin film that can be implanted in the human body for constructing the microenvironment of tendon tissue. The specific process is as follows:

[0035] 1. Take the tendon tissue and freeze it into sections with a thickness of 15 μm to obtain tendon tissue slices.

[...

Embodiment 2

[0041] 1. Take the tendon tissue and freeze it into slices with a thickness of 20 μm to obtain tendon tissue slices.

[0042] 2. Mix polydimethylsiloxane and curing agent at a mass ratio of 8:1, and stir. After mixing evenly, pour it on the tendon tissue slices, heat at 48°C, and polydimethylsiloxane Under the action of the curing agent, the siloxane is cured on the tendon tissue slices, and the tendon tissue texture will be formed after curing. After 8 hours of curing, the dimethylsiloxane is removed from the tendon tissue slices, thus forming polydimethicone. Methyl silicone mold.

[0043] 3. Mix poly(1,8-octyl citrate) containing azide group and poly(1,8-octyl citrate) containing alkyne group according to the ratio of 1:6 and pour it into polydimethylsiloxane mold , heated to 115° C. for click chemical cross-linking reaction, and after 72 hours, a click chemically modified polyoctyl citrate 1,8-octyl diester film was formed.

[0044] 4. Remove the poly(1,8-octyl citrate) ...

Embodiment 3

[0047] 1. Take the tendon tissue and freeze it into slices with a thickness of 20 μm to obtain tendon tissue slices.

[0048] 2. Take polydimethylsiloxane and curing agent, the two are mixed according to the mass ratio of 8:1, and stirred. After mixing evenly, pour it on the tendon tissue slice, heat it, and the temperature is 70 ° C. At this time The polydimethylsiloxane is cured on the tendon tissue slice under the action of the curing agent, and the tendon tissue texture will be formed after curing. After 12 hours of curing, the polydimethylsiloxane is removed from the tendon tissue slice, This forms the polydimethylsiloxane mold.

[0049] 3. Mix poly(1,8-octyl citrate) containing azide group and poly(1,8-octyl citrate) containing alkyne group according to the ratio of 1:7 and pour it into polydimethylsiloxane mold , heated to 110°C for coupling reaction, and after 84 hours, a click chemically modified poly-1,8-octyl citrate film was formed.

[0050] 4. Remove the poly-1,...

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Abstract

The invention relates to a preparing method of a biological engineering film material, particularly implantable poly(1,8-octanediol-citrate) film modified through click chemistry and a preparing method thereof. The poly(1,8-octanediol-citrate) film modified through click chemistry is prepared by subjecting azide-group-containing poly(1,8-octanediol-citrate) and alkynyl-containing poly(1,8-octanediol-citrate) to a coupling reaction on a polydimethylsiloxane mould. The poly(1,8-octanediol-citrate) film modified through click chemistry has excellent hydrophilicity so that stem cells have better adhering performance for the film, thus greatly increasing the number of stem cells used for induced differentiation. In addition, a microenvironment of muscle tendon tissues is constructed on the film, thus effectively inducing differentiation of the stem cells into muscle tendon cells.

Description

technical field [0001] The present invention relates to a method for preparing a bioengineering film material, in particular to a method for preparing an implantable film with a tendon tissue microenvironment, specifically, an implantable click chemically modified polyoctyl citrate 1,8 Thin films and methods for their preparation. Background technique [0002] The tear of the osseous tendon junction is a common injury of the sports system. This kind of injury is often caused by a sudden and violent contraction of the muscle caused by an external force, which leads to a complete or partial rupture of the tendon starting and ending points, resulting in secondary trauma. It has long plagued the field of sports medicine. problem. [0003] Stem cells are cells with differentiation potential that can produce highly differentiated functional cells. The differentiation direction of stem cells is determined based on the state of the cells themselves and microenvironmental factors. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/24A61L27/58C08G81/00C08J7/04C08L67/00
CPCA61L27/18A61L27/24A61L27/58C08G81/00C08J7/0427C08J2367/00C08J2389/00C08L89/06C08L67/00
Inventor 王冲杨健邱伟鹏林润华杨伟国
Owner THE UNIV OF HONG KONG SHENZHEN HOSPITAL
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