10 results about "Tendon cell" patented technology

The invention provides a high-porosity and anti-adhesion tendon repair material, and relates to the field of medical biomaterials.The high-porosity and anti-adhesion tendon repair material is prepared through the following steps that animal tendons are obtained, degreased and rinsed; decellularization and rinsing are carried out; performing disinfection; performing rinsing; stretching and freeze-drying: clamping two ends by using a clamp, stretching and fixing, and freeze-drying to form pores in the tendon; soaking adsorption: taking out the freeze-dried tendon, putting the tendon into a tubular mold, pouring the anti-adhesion hydrogel precursor solution into the tubular mold, and performing soaking treatment for 12-24 hours; and performing irradiation crosslinking: performing irradiation treatment on the tubular mold filled with the tendon and the anti-adhesion hydrogel precursor solution to obtain the tendon repair material. The tendon repair material not only can effectively prevent the tendon from adhering to normal tissues, but also can control the pore size of the tendon, is beneficial to infiltration and proliferation of fibroblasts and tendons, promotes blood vessel ingrowth and metabolite exchange, and provides a good basis for tissue repair and regeneration.

The invention belongs to the technical field of material chemistry and biology, and particularly relates to a composite membrane as well as a preparation method and application thereof. According to the HOFilms material, a hydrogen bond organic framework film with high quality and uniform distribution is prepared by using a mild and simple preparation method, and the biological function of the material is effectively reserved; the preparation process is simple, raw materials are easy to obtain, and factory-like large-scale production can be realized; the active achilles tendon regeneration system can be used as a physical barrier or a stent, regulates and repairs a microenvironment at a molecular level, and further forms an integrated active achilles tendon regeneration platform from mechanical support to biological treatment; the HOFilms material has the intelligent characteristic of'wet response ': the HOFilms material not only can stably exist in an in-vivo wet environment, but also can execute specific functions by utilizing or responding to moisture (such as swelling, shrinking and releasing factors) in the environment, so that the HOFilms material interacts with a dynamic physiological process; the HOFilms material has an active biological function of improving an inflammatory microenvironment and directly promoting regeneration of tendon cells, so that healing obstacles are overcome fundamentally.

The invention provides an application of miR-494-3p in preparation of a medicine for treating and repairing achilles tendon injury, relates to the technical field of biomedicine, and is technically characterized in that the application provides the application of miR-494-3p in preparation of the medicine for treating and repairing achilles tendon injury. The invention also provides an application of the miR-494-3p nanosphere hydrogel sustained-release system prepared from the miR-494-3p in preparation of a medicine for repairing achilles tendon injury, wherein the miR-494-3p nanosphere hydrogel sustained-release system is loaded with the miR-494-3p. According to the present invention, the specific embodiment verifies that the miRNA nanosphere hydrogel delivery system can effectively release the miR-494-3p, and can meet the requirement of tendon injury repair. Research finds that miR-494-3p improves tendon healing and increases the maximum tension strength of tendons by promoting tendon cell proliferation, inhibiting inflammation and apoptosis and enhancing synthesis of extracellular matrixes (especially I-type collagen) at the same time.

The present invention relates to a pharmaceutical composition for preventing or treating rotator cuff laceration comprising, as active ingredients, deteptided collagen and induced pluripotent stem cell-derived tendinocytes, and has confirmed that the induced pluripotent stem cell-derived tendinocytes have excellent adhesion and viability, can be used to produce a cell-support complex, and can be used to treat rotator cuff laceration. Especially, the compound with the detelopeptide collagen can enable cells to survive and maintain for a long time in the rotator cuff lacerated tissue. Furthermore, the apotelocollagen-induced pluripotent stem cell-derived tendinocyte complex exhibits a more excellent therapeutic effect than other complexes in terms of mechanical strength recovery and tissue regeneration promotion, and thus can be effectively used as a preparation for preventing or treating rotator cuff tear.

The present invention discloses a rotator cuff patch containing exosomes and a preparation method thereof. Nanofibers are prepared by coaxial electrospinning, which mimics the ultrastructure of the extracellular matrix. At the same time, the nanofiber structure is similar to rotator cuff tissue and can mimic rotator cuff tissue. Moreover, the nanofibers have sufficient mechanical strength and elasticity to guide tissue growth in the appropriate direction and have ideal biomedical functions. The core layer contains exosomes, which can promote the migration and fusion of osteoblasts and tendon cells, and has the effect of promoting tissue healing. At the same time, because the exosomes are encapsulated in the nanofiber core layer structure, they are released more slowly than ordinary blended nanofibers. Therefore, the core-shell structure can slow down the release of exosomes and avoid sudden release. The present invention combines the complementary advantages of high molecular polymers, extracellular vesicles (exosomes), and natural collagen in terms of composition and properties, and combines the biomimetic structure of nanofibers to prepare a biomimetic rotator cuff patch with a rotator cuff structure and promoted healing.

The invention belongs to the technical field of tissue repair, and relates to a tendon fiber patch and a preparation method thereof. The tendon fiber patch is composed of two layers of fiber structures, the lower fiber layer is in a grid shape and is formed by interweaving micron fibers, the distance between every two adjacent fibers is 0.8-1.2 mm, the included angle between every two intersected micron fibers is 80-90 degrees, and the micron fibers are made of degradable and stretchable high polymer materials and provide mechanical support; the upper fiber layer is composed of wave-shaped nanofibers arranged in an oriented mode, and the nanofibers are degradable and stretchable high polymer materials and provide a microenvironment for tendon cell regeneration. The micro-nano composite fiber patch is prepared through a pre-stretching process on the basis of immunocompetence metal ions and a medical high polymer material, and the material is good in biocompatibility and degradable. And through a pre-stretching process, the orientation of the fibers is enhanced.

This invention discloses the application of the transcription factor Creb3l1 in the preparation of drugs that promote the repair of injured tendons in the elderly, belonging to the field of biomedical technology. This invention identifies a transcription factor, Creb3l1, which, according to software prediction, can simultaneously bind to the promoter regions of the Col1a1 and Sparc genes, promoting the transcription and translation of Col1a1 and Sparc, thereby improving the efficacy of tendon injury repair in the elderly. In vivo experiments have confirmed that overexpression of Creb3l1 can significantly improve tendon gliding function, reduce peritendinous adhesions, and significantly enhance tendon healing strength and Young's modulus, promoting the healing of injured tendons. In vitro experiments further show that overexpression of Creb3l1 can significantly enhance the proliferative activity of human tendon cells. This invention provides a novel and effective solution for the clinical treatment of tendon injuries in the elderly, possessing promising clinical application prospects.

The invention discloses a method for inducing and differentiating induced pluripotent stem cells into tendon cells by using a gene regulation and control system, which comprises the following steps: S1, constructing doxycycline-induced insertion template plasmids for conditional expression of tendon-specific genes: S1.1, by taking PB-TRE-EGFP-EF1a-rtTA as initial skeleton plasmids, carrying out induction and differentiation on the insertion template plasmids, so as to obtain the tendon cells; constructing an insertion donor vector of a DNA sequence with a transcriptional activation type Tet-on system for controlling and expressing MKX; s1.2, by taking PB-TRE-EGFP-EF1a-rtTA as an initial skeleton plasmid, constructing an insertion donor vector of a DNA (Deoxyribose Nucleic Acid) sequence with a transcriptional activation type Tet-on system for controlling and expressing SCX (Sequence Content Expression); s2, culturing the iPSCs, and screening an electrotransfection cell strain and a stably transfected cell strain; and S3, inducing the iPSCs to be differentiated to the iPSCs-tencytes.

The present application belongs to the field of material chemistry and biology technology, and particularly relates to a composite film and a preparation method and application thereof. The HOFilms material of the present application uses a mild and simple preparation method to prepare a high-quality, uniformly distributed hydrogen-bonded organic framework film, effectively retaining the biological function of the material; the production process is simple, raw materials are easy to obtain, and the material can be produced in large scale in a factory; the material can be used as a physical barrier or a support to regulate the repair microenvironment at the molecular level, and further form an integrated active Achilles tendon regeneration platform from mechanical support to biological treatment; the HOFilms material has a "wet response" intelligent property: not only can it stably exist in a wet environment in vivo, but also can utilize or respond to moisture in the environment (such as swelling, shrinking, releasing factors) to perform specific functions, thereby interacting with dynamic physiological processes; the HOFilms material itself has an active biological function, that is, improving the inflammatory microenvironment and directly promoting the regeneration of tendon cells, thereby fundamentally overcoming the healing barrier.

The present invention provides a second use of mitochondria, which can cure a tendon injury-related disease and prevent a disease caused by a tendon injury. Specifically, the mitochondria disclosed in the present invention have the effect of repairing injured tendon cells and accelerating the healing of the tendon cells. Therefore, by administering a predetermined amount of mitochondria or a composition containing a predetermined amount of mitochondria to a part with a tendon injury, wound healing of the part with the tendon injury can be promoted, thus achieving the effect of repairing the injured tendon and further preventing a joint disease caused by the tendon injury or inflammation.