37 results about "Tendon cell" patented technology

Connective tissue, including neo-tendons and ligaments, has been constructed using biodegradable synthetic scaffolds seeded with tenocytes. The scaffolds are preferably formed from biodegradable fibers formed of a polymer such as polyglycolic acid-polylactic acid copolymers, and seeded with cells isolated from autologous tendon or ligament by means of enzymatic digestion or direct seeding into tissue culture dishes from explants. The cell polymer constructs are then surgically transplanted to replace missing segments of functioning tendon or ligament.

Connective tissue, including neo-tendons and ligaments, has been constructed using biodegradable synthetic scaffolds seeded with tenocytes. The scaffolds are preferably formed from biodegradable fibers formed of a polymer such as polyglycolic acid-polylactic acid copolymers, and seeded with cells isolated from autologous tendon or ligament by means of enzymatic digestion or direct seeding into tissue culture dishes from explants. The cell polymer constructs are then surgically transplanted to replace missing segments of functioning tendon or ligament.

A device has been developed to augment the rotator cuff tendon tissue as it proceeds in healing. The device has two purposes: to provide initial stability to the rotator cuff repair site to allow early mobilization of the upper extremity of the patient, and to allow for reinforcement of rotator cuff tendon repairs to increase the likelihood of successful rotator cuff tendon repairs. The device consists of an inter-connected, open pore structure that enables even and random distribution and in-growth of tendon cells. The braided structure allows for distribution of mechanical forces over a larger area of tissue at the fixation point(s).

A multi-region device for repair, regeneration or reconstruction in articular tissue injury such as torn ligaments and tendons is provided. The device comprises at least one degradable material and biocompatible non-degradable polymeric fiber-based material, in a three-dimensional braided scaffold. The two end sections are designed for attachment of the device at the site of implantation and are designed to allow bone cell ingrowth, and one or more middle regions are designed to allow ligament or tendon cell ingrowth.

It is intended to provide a pluripotent cell having the following properties: (1) being contained in a mixed-cell type population obtained by enzymatic treatment of the tissue collected from an animal; (2) being contained in a sedimented cell population obtained by centrifugating the mixed-cell type population of the property (1); (3) selectively proliferating by culturing in a medium containing 2% (v / v) or lower serum and 1 to 100 ng / ml of fibroblast growth factor-2; and (4) being differentiated into cells having the characteristics of adipocytes, osteoblasts, chondrocytes, tendon cells, myocardial cells, myoblasts, neurocytes or vascular endothelial cells by adjusting the culture condition; cells having differentiated from the above cell; and a method of conveniently obtaining the pluripotent cell in a large amount.

A device has been developed to augment the rotator cuff tendon tissue as it proceeds in healing. The device has two purposes: to provide initial stability to the rotator cuff repair site to allow early mobilization of the upper extremity of the patient, and to allow for reinforcement of rotator cuff tendon repairs to increase the likelihood of successful rotator cuff tendon repairs. The device consists of an inter-connected, open pore structure that enables even and random distribution and in-growth of tendon cells. The braided structure allows for distribution of mechanical forces over a larger area of tissue at the fixation point(s).

The invention discloses a preparation method of a tendon conjunction bone decellularization material of a natural tissue source. The heel tendon conjunction bone tissue of any size of a mammal is treated by normal saline containing protease inhibitor, PBS containing protease inhibitor, a PBS buffer solution containing Triton X, a PBS buffer solution containing SDS and a PBS buffer solution containing DNA enzyme, and the tendon conjunction bone decellularization material is obtained. Complete decellularization treatment can be carried out on tendon cells and bone cells on tendon conjunction bone tissue and cartilage cells on the interface at the same time, conditions are mold, no ECM damage is caused, the process is rapid and stable, and the obtained tendon conjunction bone material has the advantages of being good in biocompatibility, strong in plasticity, high in biomechanical strength and the like, and can be used for repairing tendon degenerative diseases caused by various etiological factors clinically, tendon severe tear and tendon conjunction bone regeneration and repair obstacles.

The pneumatic Achilles sleeve assembly comprises a sleeve and at least one strap for fastening the sleeve around the ankle. The sleeve assembly positions an arch cell which contains a dynamic volume of air within the sleeve under a human foot. The arch cell is fabricated from a flexible material and is in communication with a conduit member. Upon application of external pressure to the arch cell, air is expelled from said air cell through said conduit member. The sleeve assembly also positions a tendon cell which contains a dynamic volume of air within the sleeve against the Achilles tendon. The tendon cell is in communication with the arch cell via the conduit member. The tendon cell is also fabricated from a flexible material. Upon the expelling of air from said arch cell, the air enters through the conduit member into the tendon cell which exerts a greater pressure against the Achilles tendon.

The invention discloses a preparation method of a cell preparation for promoting the regeneration and the repair of traumas. The preparation method comprises the steps of shearing and digesting heel tendon tissues, carrying out centrifuging to remove supernate, adding an obtained tendon stem cell population into a culture solution, carrying out resuspension and filtering, inoculating the culture solution into a culture plate for culturing, mixing the culture solution with platelet rich plasma, and adding normal saline, so as to prepare a tendon stem cell population. Tendon stem cells are separated and cultured, tendon stem cells cultured in vitro are mixed with the platelet rich plasma to prepare the cell preparation, the platelet rich plasma contains a large number of growth factors such as PDGF, EGF, TGF-beta1 and IGF which have great promotion effects to the repair of tendons, the platelet rich plasma is capable of promoting the propagation of tendon cells and the expression of collagens I and III in vivo, and the tendon cells and the collagens I and III are key components of the tendons, so that an experimental result has a certain guiding effect to the repair of tendon injury of a human body.

The invention relates to the field of regeneration and repair of tissues and organs and in particular relates to a method for preparing a tendon repair material and provides a novel path for tendon defect repair. The tendon repair material disclosed by the invention is prepared by binding animal-derived decellularized achilles tendon material and specific tendon seed cells. The achilles tendon material selectively remains various expected beneficial components contributing to tendon regeneration while eliminating immunogenicity of heterologous tissues, while the seed cells are in-vitro controllable induced receptor tendon cells of exogenous growth factors. According to the combination of the achilles tendon material and the seed cells, an effect of regenerating tissue-engineered tendons iseffectively achieved, the clinical applicability is high, and the tendon repair material is preferentially but not limited to be applied to tendon repair.

The invention relates to the field of biological material, and particularly relates to an acellular matrix tendon anti-adhesion film and a preparation method thereof. The method disclosed by the invention is characterized in that mammalian tendons are used, and an acellular matrix tendon anti-adhesion film is prepared by a process of raw material treatment, decellularization, matrix dissolution, low-temperature drying film formation, neutralization water washing, and the like. The acellular matrix tendon anti-adhesion film provided by the invention eliminates the immunogenicity of heterologousand foreign tissues, retains the active components of the tendon extracellular matrix, directly wraps a tendon injury part, provides a good microenvironment for tendon repair, prevents adhesion of tendons to surrounding tissues, and promotes tendon repair. In addition, the method for preparing the tendon anti-adhesion film has low cost.

The present invention relates to methods for preparing bio scaffolds useful in the repair of tears. More specifically, the invention relates to a method of treating rotator cuff tear in a mammalian subject in need thereof comprising the steps of: (i) selectively expanding tenocytes in vitro in culture medium comprising insulin or a functional derivative and a glucocorticoid or a glucocorticoid-like molecule to produce a culture of expanded tenocytes; (ii) seeding a bioscaffold with said expanded tenocytes to produce a tenocyte seeded bioscaffold; and (iii) implanting said tenocyte seeded bioscaffold proximal to the rotator cuff tear. The present invention also relates to a bioscaffold comprising cells, wherein more than 80% of said cells are tenocytes.

A histo-engineered muscle tendon is composed of a pharmacologically acceptable biodegradable material, muscle tendon cells inoculated to said biodegradable material, and biologic membrane for wrapping said biodegradable material. It can be used to repair muscle tendon.

The invention relates to the technical field of stem cells, in particular relates to a cryopreservation solution for tendon stem cells from an achilles tendon of a human and a preparation method of the cryopreservation solution. The cryopreservation solution for the tendon stem cells from the achilles tendon of the human consists of a conditional culture solution and dimethyl sulfoxide. The preparation method of the cryopreservation solution for the tendon stem cells from the achilles tendon of the human comprises the following steps: (1) cell culture; (2) preparation of the conditional culture solution; and (3) mixing of the conditional culture solution and dimethyl sulfoxide at a volume ratio of 9 to 1. According to the cryopreservation solution, the cost of cell cryopreservation can be reduced to a certain extent, and introduction of heterologous substances can be avoided; compared with the conventional cell cryopreservation solution, the cryopreservation solution disclosed by the invention is higher in clinical safety. The cryopreservation solution for the tendon stem cells from the achilles tendon of the human is lower in cell damage effect in a cell cryopreservation process; meanwhile, the cell survival rate is higher; the cryopreservation effect is obviously higher than that of the conventional cell cryopreservation solution; the cryopreservation solution can be used for long-time storage and application of the tendon stem cells from the achilles tendon of the human.

The invention discloses a compound material for the internal fixation of tendons carrying transgenic cells. The compound material is formed by compounding a non-degradable tendon surface internal fixation polyester material layer as the outer layer and a pharmacologically acceptable biodegradable porous substrate film material layer as the inner layer, wherein, the inner layer adopts the biodegradable substrate film as the base material obtained through the surface modification treatment of substances capable of improving the hydrophilicity and cell affinity thereof and the inoculation of tendon cells subjected to the transfection of genes carrying the related cell factors for promoting the healing of tendons. The preparation method of the compound material comprises the following steps: firstly, preparing the non-degradable tendon surface internal fixation polyester material compound and the surface-modified biodegradable substrate film; then, preparing the transgenic tendon cells, and inoculating the transgenic tendon cells onto the biodegradable substrate film; and finally compounding to obtain the compound material of the invention. The compound material prepared by the invention has the advantages of good biocompatibility and higher tensile strength and is capable of stabilizing the internal environment of the tendons and promoting the healing of the tendon injury.

A multi-region device for repair, regeneration or reconstruction in articular tissue injury such as torn ligaments and tendons is provided. The device comprises at least one degradable material and biocompatible non-degradable polymeric fiber-based material, in a three-dimensional braided scaffold. The two end sections are designed for attachment of the device at the site of implantation and are designed to allow bone cell ingrowth, and one or more middle regions are designed to allow ligament or tendon cell ingrowth.

The invention relates to the technical field of tissue engineering decellularization, in particular to a method for preparing tendon / ligament acellular matrix materials by an ultrasonic concussion technology. According to the method, the ultrasonic concussion technology is used for decellularization, mechanical structure units and cell attachment structures inherent in the tendon collagen fibers or fiber bundles are retained, and the good mechanical performance and the good biocompatibility are achieved. A tendon extracellular matrix decellularized by the ultrasonic concussion technology is used, in addition to application of repair and reconstruction of the ligament and the tendon, the tendon extracellular matrix can be further widely applied to other biomedical fields since the characteristics of a fibrous material is achieved, and the social required quantity is great.

The invention relates to a method for preparing a tissue engineering-oriented artificial tendon transplant based on an artificial tendon scaffold material. The method comprises the following steps: inoculating mesenchymal stem cells which are cultured to the third generation into a culture solution to culture; absorbing the culture solution, and adding a composite culture solution to infect for 4 hours; replenishing the composite culture solution to continually infect for 24 hours; absorbing the composite culture solution, and filling a culture solution containing 10 percent FBS (Fetal Bovine Serum) to infect continually for 20 hours to obtain cell sap carrying a report gene of GFP (Green Fluorescent Protein); transferring cells for expressing the GFP into the culture solution to culture in order to obtain seed cells carrying GFP-BMSCs; soaking an artificial tendon scaffold into a low-sugar DMEM (Dulbecco Modified Eagle Medium) to culture for 24 hours, planting GFP-BMSC cells onto the scaffold to culture continually for 48 hours, and transferring into tendon cell induced liquid to culture for 1-2 weeks in order to obtain an artificial tendon transplant. The prepared tissue engineering-oriented artificial tendon transplant can be planted into a human body to repair injured tendons.

The invention relates to a preparing method of a biological engineering film material, particularly implantable poly(1,8-octanediol-citrate) film modified through click chemistry and a preparing method thereof. The poly(1,8-octanediol-citrate) film modified through click chemistry is prepared by subjecting azide-group-containing poly(1,8-octanediol-citrate) and alkynyl-containing poly(1,8-octanediol-citrate) to a coupling reaction on a polydimethylsiloxane mould. The poly(1,8-octanediol-citrate) film modified through click chemistry has excellent hydrophilicity so that stem cells have better adhering performance for the film, thus greatly increasing the number of stem cells used for induced differentiation. In addition, a microenvironment of muscle tendon tissues is constructed on the film, thus effectively inducing differentiation of the stem cells into muscle tendon cells.

The invention relates to the technical field of stem cells, in particular to a culture medium and application thereof and a method for inducing tendon stem cells to differentiate into bone cells. According to the culture medium and application thereof and the method for inducing the tendon stem cells to differentiate into the bone cells, FBS, vitamin C, dexamethasone, beta- glycerophosphate and BMP-2 are added into the basic culture medium as an induction culture solution for tendon stem cell osteogenic differentiation. The number of tendon stem cells which are differentiated into bone cells is increased and the differentiation time is shortened. An experiment shows that the culture solution is used for inducing the tendon stem cells, and the characteristics of bone cells can appear within21days, the cell morphology is changed and the number of alizarin red staining cells is increased; and after 28 days of induction, the morphology of the cells is changed gradually, the cells are in apolygonal shape, calcified nodules with mineral deposits exist in the intercellular stroma, and alizarin red staining is positive.

The present invention relates to methods for preparing bio scaffolds useful in the repair of tears. More specifically, the invention relates to a method of treating rotator cuff tear in a mammalian subject in need thereof comprising the steps of: (i) selectively expanding tenocytes in vitro in culture medium comprising insulin or a functional derivative and a glucocorticoid or a glucocorticoid-like molecule to produce a culture of expanded tenocytes; (ii) seeding a bioscaffold with said expanded tenocytes to produce a tenocyte seeded bioscaffold; and (iii) implanting said tenocyte seeded bioscaffold proximal to the rotator cuff tear. The present invention also relates to a bioscaffold comprising cells, wherein more than 80% of said cells are tenocytes.

In vitro process for the proliferation or culture of conjunctive tissue cells selected from the group consisting of chondrocytes, osteoblasts, chondroprogenitor cells, osteoprogenitor cells, tenocytes and ligament cells comprising the step of contacting said cells with an aminosugar selected from the group consisting of mannosamine, N-acetylmannosamine, mannosamine salts and their mixtures. The present invention also relates to compositions which comprise the aminosugar in combination with the cells. These compositions are useful for the treatment of cartilage, bone, tendon and ligament lesions or defects, bone mass losses and osteochondral defects or lesions.

A method for cultivating tendon cells from non-embryonic pluripotent cells of mesenchymal origin is described, wherein the isolated cells are cultivated in a culture medium under standard culture conditions in a culture vessel. In order to increase the collagen secretion, it is proposed that before their complete confluence, the cells are on the one hand further cultivated in a culture medium mixed with ascorbic acid and / or ascorbic acid-2-phosphate in a concentration of 25 to 75 μg / ml and on the other hand, subjected to hyperosmolar treatment in a culture medium whose osmolarity is adjusted to 350 to 500 mosmol / l.

The invention discloses a manufacturing method suitable for a mouse tendon tissue paraffin section. After the tendon tissue is placed into the fixing liquid to be fixed, the tendon tissue is transferred into the preservation liquid to be preserved, dehydrated, permeated, embedded in paraffin and sliced, and the tendon tissue paraffin section is obtained, wherein the preservation fluid is ethanol or a mixture of ethanol and glacial acetic acid. The method is suitable for manufacturing the mouse tendon tissue paraffin section, the mouse tendon tissue in the prepared mouse tendon tissue paraffinsection is complete in structure, tendon cells are arranged in order, tendons are not broken, a very good section effect is obtained and the success rate reaches 95% or above; in addition, the manufacturing method of the mouse tendon tissue paraffin section is simple, convenient, rapid, economical and efficient and has a wide application prospect.

The present invention relates to a human skin-derived fibroblast having a tendon regeneration effect, and a pharmaceutical composition for tendon regeneration and treatment comprising the same as an active ingredient, the fibroblast derived from the same kind of skin being similar to a tendon cell in cell morphology, according to the present invention, the expression level of the waveform protein as the fibroblast marker is high, a large amount of extracellular matrix protein is expressed, and the synthesis of collagen can be increased by acting on TGF-beta and ERK signal transmission mechanisms, such that the damaged tendon can be effectively regenerated.

The invention relates to the technical field of cell culture, and in particular relates to a culture medium and a method for inducing adipose-derived stem cells to differentiate into tendon cells. The culture medium comprises GDF-7, prepared rehmannia root aqueous extract and a serum-free culture medium. The culture medium provided by the invention can remarkably improve the ability of differentiating the adipose-derived stem cells into tendon cells; the prepared rehmannia root aqueous extract is utilized to culture the adipose-derived stem cells, so that the phenotype and the characteristics of the adipose-derived stem cells further can be kept while cell proliferation is promoted; a growth differentiating factor GDF-7 is used as an inductor, so that the effect is single, and the complex components of blood plasma are greatly reduced, and therefore, the adipose-derived stem cells are differentiated into the tendon cells in a directional manner, and the risk and the immunogenicity of animal serum application can be reduced.

The present invention provides a tissue engineering tendon graft. It includes: (a). pharmaceutically-acceptable bio-degradable material.; (b) seed cell, said seed cell can be inoculated in the described bio-degradable material and is selected from (i) fibroblast; and (ii), mixture of fibroblast and tendon cell and / or bone marrow matrix stem cell according to the ratio of 1:10-10000:1; and (c) bio-membrane, the exterior of said bio-degradable material is covered with said bio-membrane. Said invented graft can beused for effectively curing musculotendinous defect. Said invention also provides its preparation and application.

The invention discloses an anti-adhesion tendon material and a preparation method thereof. The tendon material is composed of regulatory T lymphocyte exosomes and tendons, and is prepared by compounding the T lymphocyte exosomes and the tendons and then freeze-drying the T lymphocyte exosomes and the tendons. The tendon tissue of the prepared composite Treg cell exosome is exposed in body fluid, nutrient substances can be fully obtained, the growth of implanted tendon cells into the tendon tissue can be accelerated, the regeneration and reconstruction time of the tendon tissue can be shortened, the Treg cells can be cultured on a large scale, the Treg cell exosome is easy to obtain, and after the Treg cell exosome and the tendon material are freeze-dried, the preparation process is simple, and the preparation cost is low. The tendon material can be stored for a long time under the refrigeration condition or the room temperature condition, the shelf life is long, and the prepared tendon material is convenient to use and does not need physical isolation.