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Manufacturing method suitable for mouse tendon tissue paraffin section

A production method and paraffin section technology, applied in the field of biological tissue production, can solve the problems of hard touch, incomplete tissue, white tissue, etc., and achieve the effects of complete tissue structure, inhibition of shrinkage, and strong penetration ability.

Active Publication Date: 2020-05-19
武汉赛维尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the gaps in the existing method for making paraffin sections of rat tendon tissue, and to solve the problem of penetrating liquid due to the compact structure of rat tendon tissue. The tissue itself is white and feels hard to the touch. When using conventional paraffin embedding, the tissue tends to turn white, the tissue cannot be sliced, and the success rate is low. A paraffin wax suitable for rat tendon tissue is provided. How to slice

Method used

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  • Manufacturing method suitable for mouse tendon tissue paraffin section
  • Manufacturing method suitable for mouse tendon tissue paraffin section
  • Manufacturing method suitable for mouse tendon tissue paraffin section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Paraffin Sections of Rat Tendon Tissue

[0038] The making of mouse tendon tissue paraffin section comprises the following steps:

[0039] Put the mouse tendon tissue into the fixative solution (a mixture of paraformaldehyde with a mass concentration of 4%, a volume fraction of 95% and a mixture of glacial acetic acid with a volume fraction of 5%) for 36 hours, and then transfer to a preservation solution (a volume fraction of Store in a mixture of 70% ethanol and 5% glacial acetic acid) for 1 day, then put the tissue directly into graded ethanol and use an automatic dehydrator to dehydrate and infiltrate, put it into the tissue embedding frame, add melted paraffin, and paraffin after natural cooling After solidification and forming, the tissue wax block was sliced ​​into 3 μm sections with a paraffin microtome. After sectioning, the slices were spread in distilled water at 40° C. and baked to obtain the paraffin slices of the tendon tissue.

Embodiment 2

[0040] Example 2 Preparation of Paraffin Sections of Rat Tendon Tissue

[0041] The making of rat tendon tissue paraffin section comprises the following steps:

[0042] The rat tendon tissue was fixed in a fixative solution (a mixture of 3% mass concentration, 90% volume fraction of paraformaldehyde and 10% volume fraction of glacial acetic acid) for 24 hours, and then transferred to a preservation solution (volume fraction of The mixture of 68% ethanol and glacial acetic acid with a volume fraction of 7%) was preserved for 2 days, and then the tissue was directly dehydrated and infiltrated in graded ethanol with an automatic dehydrator, put into a tissue embedding frame, added melted paraffin, and paraffin wax after natural cooling After solidification and forming, the tissue wax block was sliced ​​into 3 μm sections with a paraffin microtome. After sectioning, the slices were spread in distilled water at 40° C. and baked to obtain the paraffin slices of the tendon tissue.

Embodiment 3

[0043] Example 3 Preparation of Paraffin Sections of Rat Tendon Tissue

[0044] The making of rat tendon tissue paraffin section comprises the following steps:

[0045] The rat tendon tissue was fixed in a fixative solution (a mixture of 5% mass concentration, 90% volume fraction of paraformaldehyde and 10% volume fraction of glacial acetic acid) for 48 hours, and then transferred to a preservation solution (volume fraction of Store in a mixture of 72% ethanol and 3% glacial acetic acid) for 2 days, then put the tissue directly into graded ethanol and dehydrate and infiltrate it with an automatic dehydrator, put it into the tissue embedding frame, add melted paraffin, and paraffin after natural cooling After solidification and forming, the tissue wax block was sliced ​​into 3 μm sections with a paraffin microtome. After sectioning, the slices were spread in distilled water at 40° C. and baked to obtain the paraffin slices of the tendon tissue.

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Abstract

The invention discloses a manufacturing method suitable for a mouse tendon tissue paraffin section. After the tendon tissue is placed into the fixing liquid to be fixed, the tendon tissue is transferred into the preservation liquid to be preserved, dehydrated, permeated, embedded in paraffin and sliced, and the tendon tissue paraffin section is obtained, wherein the preservation fluid is ethanol or a mixture of ethanol and glacial acetic acid. The method is suitable for manufacturing the mouse tendon tissue paraffin section, the mouse tendon tissue in the prepared mouse tendon tissue paraffinsection is complete in structure, tendon cells are arranged in order, tendons are not broken, a very good section effect is obtained and the success rate reaches 95% or above; in addition, the manufacturing method of the mouse tendon tissue paraffin section is simple, convenient, rapid, economical and efficient and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological tissue preparation. More specifically, it relates to a method for making paraffin sections of rat tendon tissue Background technique [0002] Tendon refers to the connective tissue fiber cord at the end of muscle. It has a dense tissue structure, is tougher than muscle and is smaller in volume. It is composed of parallel and neatly arranged bundles of collagen fibers. There are tendon cells arranged in parallel along its long axis between the bundles. The tendon does not contract. ability. Such dense tissues include the patellar ligament and Achilles tendon. Tendon morphological structure is a very intuitive detection index in the repair of tendon pathological injury in trauma surgery; therefore, tendon tissue section is a more commonly used histological method. However, due to the relatively dense tissue structure of the tendon, the resistance to liquid penetration is relatively high, the t...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/2813
Inventor 张高英黄静潘亮赵红洲
Owner 武汉赛维尔生物科技有限公司
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