Manufacturing method suitable for mouse tendon tissue paraffin section

A production method and paraffin section technology, applied in the field of biological tissue production, can solve the problems of hard touch, incomplete tissue, white tissue, etc., and achieve the effects of complete tissue structure, inhibition of shrinkage, and strong penetration ability.
CN111175100AActive Publication Date: 2020-05-19武汉赛维尔生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
武汉赛维尔生物科技有限公司
Publication Date
2020-05-19

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Abstract

The invention discloses a manufacturing method suitable for a mouse tendon tissue paraffin section. After the tendon tissue is placed into the fixing liquid to be fixed, the tendon tissue is transferred into the preservation liquid to be preserved, dehydrated, permeated, embedded in paraffin and sliced, and the tendon tissue paraffin section is obtained, wherein the preservation fluid is ethanol or a mixture of ethanol and glacial acetic acid. The method is suitable for manufacturing the mouse tendon tissue paraffin section, the mouse tendon tissue in the prepared mouse tendon tissue paraffinsection is complete in structure, tendon cells are arranged in order, tendons are not broken, a very good section effect is obtained and the success rate reaches 95% or above; in addition, the manufacturing method of the mouse tendon tissue paraffin section is simple, convenient, rapid, economical and efficient and has a wide application prospect.
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Description

technical field

[0001] The invention belongs to the technical field of biological tissue preparation. More specifically, it relates to a method for making paraffin sections of rat tendon tissue Background technique

[0002] Tendon refers to the connective tissue fiber cord at the end of muscle. It has a dense tissue structure, is tougher than muscle and is smaller in volume. It is composed of parallel and neatly arranged bundles of collagen fibers. There are tendon cells arranged in parallel along its long axis between the bundles. The tendon does not contract. ability. Such dense tissues include the patellar ligament and Achilles tendon. Tendon morphological structure is a very intuitive detection index in the repair of tendon pathological injury in trauma surgery; therefore, tendon tissue section is a more commonly used histological method. However, due to the relatively dense tissue structure of the tendon, the resistance to liquid penetration is relatively high, the t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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