Method for preparing tissue engineering-oriented artificial tendon transplant based on artificial tendon scaffold material

A technology of artificial tendon and tissue engineering, which is applied in the field of preparation of tissue engineered artificial tendon grafts, and can solve the problems of lack of

Active Publication Date: 2016-11-09
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The currently developed tissue-engineered tendon does not have this function of in vitro observation

Method used

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  • Method for preparing tissue engineering-oriented artificial tendon transplant based on artificial tendon scaffold material
  • Method for preparing tissue engineering-oriented artificial tendon transplant based on artificial tendon scaffold material
  • Method for preparing tissue engineering-oriented artificial tendon transplant based on artificial tendon scaffold material

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Embodiment 1

[0042] 1. Preparation of seed cells

[0043] (1) Preparation of bone marrow mesenchymal stem cells: Bone marrow mesenchymal stem cells (BMSCs) cultured to the third passage were inoculated on DMEM low-glucose medium in a 6-well plate, and the inoculated cell concentration was 1×10 6 / ml, cultured in a cell culture incubator. Cultured until the cells reached 55% confluency.

[0044] (2) BMSCs transfected with lentivirus LV-GFP: Aspirate the DMEM low-sugar culture medium with BMSCs with a confluence rate of 50%, then add the compound culture medium, and set the temperature in the cell culture incubator at 37°C and 5% CO 2 Concentration culture conditions, infection for 4 hours; at 4 hours, supplement the compound culture solution to the original volume, and continue to infect for 24 hours; absorb the compound culture solution, replace it with fresh DMEM low-sugar culture solution containing 10% FBS and continue culturing for 20 hours, after digestion, The BMSCs liquid with the...

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Abstract

The invention relates to a method for preparing a tissue engineering-oriented artificial tendon transplant based on an artificial tendon scaffold material. The method comprises the following steps: inoculating mesenchymal stem cells which are cultured to the third generation into a culture solution to culture; absorbing the culture solution, and adding a composite culture solution to infect for 4 hours; replenishing the composite culture solution to continually infect for 24 hours; absorbing the composite culture solution, and filling a culture solution containing 10 percent FBS (Fetal Bovine Serum) to infect continually for 20 hours to obtain cell sap carrying a report gene of GFP (Green Fluorescent Protein); transferring cells for expressing the GFP into the culture solution to culture in order to obtain seed cells carrying GFP-BMSCs; soaking an artificial tendon scaffold into a low-sugar DMEM (Dulbecco Modified Eagle Medium) to culture for 24 hours, planting GFP-BMSC cells onto the scaffold to culture continually for 48 hours, and transferring into tendon cell induced liquid to culture for 1-2 weeks in order to obtain an artificial tendon transplant. The prepared tissue engineering-oriented artificial tendon transplant can be planted into a human body to repair injured tendons.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering. Specifically, it relates to a method for preparing a tissue-engineered artificial tendon graft based on an artificial tendon scaffold material. Background technique [0002] According to research reports: tendon injuries often occur in daily work and sports, and there are more than 30 million tendon injuries worldwide every year. It is difficult for the repaired tendon to return to its pre-injury functional state by traditional treatment methods. The treatment of tendon injuries has also become the focus of sports medicine research. With the development of tissue engineering technology, tissue engineered tendon provides a way to solve this problem. Compared with the traditional treatment methods for tendon injuries, there are no longer problems such as loss of autologous donor site function and rejection of allografted tendons. [0003] The principle of tissue engineered tendon is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/50
CPCA61L27/20A61L27/3834A61L27/50A61L2430/10C08L1/02
Inventor 黄镇黄祖新周文浩
Owner FUJIAN NORMAL UNIV
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