Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue engineered tendons and ligaments

a technology of tendons and ligaments, applied in the field of tissue engineered tendons and ligaments, can solve the problems of disordered structure which imparts this ability to the connective tissue, tendon defects, and proves difficult problems for orthopedic surgery and hand surgery, and achieves the effect of moderate tensile strength

Inactive Publication Date: 2005-03-17
VACANTI CHARLES A +5
View PDF99 Cites 104 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When damaged, the orderly structure which imparts this ability to the connective tissue is disrupted and usually does not heal to yield a fully functional tissue.
Tendon defects, regardless of their origin, often prove to be difficult problems for orthopedic surgery and hand surgery.
Autogenous tendon usage is limited by availability and donor site morbidity.
Homo- or heterografts, though, suffer from a scarcity of supply, increased susceptibility to infection, and concerns about possible transmission of infectious agents.
The original structure of the tendons is not maintained, however, and the repaired tendon lacks strength and flexibility.
Artificial prostheses, although not infectious, are also susceptible to infection, extrusion, and uncertain long-term immunologic interactions with the host.
Moreover, prostheses cannot adapt to environmental stresses as do living tendon tissue and have a high incidence of adhesive breakdown at their interface with the host.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue engineered tendons and ligaments
  • Tissue engineered tendons and ligaments

Examples

Experimental program
Comparison scheme
Effect test

example 1

Construction of Neo-tendon

Method and Materials

[0035] Sheets approximately 100 microns thick composed of an embossed non-woven mesh of polyglycolic acid with interfiber interstitial spacing averaging 75 to 100 microns in diameter, arranged as either a random array of fibers, or as fibers in parallel (Dexon, Davis and Geck), were cut into pieces approximately 0.4 cm×4 cm and set aside. Tendon was obtained from the shoulder of newborn calves within six hours of sacrifice. The tendons were diced into pieces approximately 0.5 cm×0.5 cm and placed in a sterile 50 ml conical tube. The tendon pieces were washed twice with Dulbecco's phosphate buffered saline (PBS) (Gibco, Grand Island, N.Y.). A sterile 0.396 Collagenase solution was prepared by mixing 75 mg of type collagenase (Worthington, Freehold, N.J.) with 25 ml of Hamm's F-12 medium (Gibco, Grand Island, N.Y.). The tendon fragments were incubated in the collagenase solution for 12-16 hours at 37° C. on a shaker. After digestion, the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
thickaaaaaaaaaa
Login to View More

Abstract

Connective tissue, including neo-tendons and ligaments, has been constructed using biodegradable synthetic scaffolds seeded with tenocytes. The scaffolds are preferably formed from biodegradable fibers formed of a polymer such as polyglycolic acid-polylactic acid copolymers, and seeded with cells isolated from autologous tendon or ligament by means of enzymatic digestion or direct seeding into tissue culture dishes from explants. The cell polymer constructs are then surgically transplanted to replace missing segments of functioning tendon or ligament.

Description

BACKGROUND OF THE INVENTION [0001] The present invention is generally in the area of forming new tissues by implantation of appropriate cells on a polymer matrix, and is specifically directed towards construction of new tendons and ligaments. [0002] Tissues connecting bones and muscles are collectively referred to herein as “connective tissue”. Tendons are tissues which attach muscles to bones; aponeuroses are sheet-like tendons connecting one muscle with another or with bones; ligaments hold bones together at joints. Tendons and ligaments are elongated, cylindric structures formed of dense connective tissue, adapted for tension in one direction, with fibers having an orderly, parallel arrangement. The most common variety of dense regularly arranged connective tissue has a predominance of collagenous (white) fibers arranged in bundles. Fibroblasts are placed in rows between the bundles. The tissue is silvery white, tough, yet somewhat pliable. The collagen bundles of the tendons agg...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61F2/08A61L27/18A61L27/38A61L27/58
CPCA61F2/08A61L27/18A61L27/3604A61L27/3662A61L27/3804A61L27/3817A61L27/58A61L27/3843A61L27/386C08L67/04A61L2430/10
Inventor VACANTI, CHARLES A.CAO, YI LINLANGER, ROBERT S.VACANTI, JOSEPH P.PAIGE, KEITHUPTON, JOSEPH
Owner VACANTI CHARLES A
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products