Method for preparing tendon repair material
A technology for repairing materials and tendons, applied in the field of preparation of tendon repair materials, to achieve the effect of eliminating immunogenicity, good biocompatibility, and high clinical practical value
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Embodiment 1
[0025] Example 1 Preparation of tendon repair material using porcine Achilles tendon
[0026] (1) Take freshly slaughtered pig Achilles tendon under sterile conditions, use surgical scissors to remove fat fragments and other impurities on the Achilles tendon, rinse blood stains and other dirt with PBS buffer, and then soak and disinfect with 0.5% peracetic acid solution 1h, the treated Achilles tendon tissue was frozen at -40°C for 8h, then pulverized with a homogenizer, and the pulverized porcine Achilles tendon granules were extracted in 0.15 wt% trypsin in PBS buffer solution for 1.5h , extracted in PBS buffer solution of 0.15% EDTA for 1.5h, then decellularized in PBS buffer solution of 1.5wt%TBP for 3.5h to remove cellular components, and then treated with 150μg / ml ribonuclease and 150μg / ml Deoxyribonuclease was treated with PBS buffer solution for 1.5 hours to remove residual nucleic acid, adjust the pH to the pH value of human body fluid, and wash with PBS buffer soluti...
Embodiment 2
[0029] Example 2 Preparation of tendon repair material using human Achilles tendon as material
[0030](1) According to the relevant procedures and permits, the Achilles tendon of the donor was removed according to the law, and the fat fragments and other impurities on the Achilles tendon were removed with surgical scissors under aseptic conditions, and the blood stains and other dirt were rinsed with PBS buffer, and then 0.1% filter solution was used. The oxyacetic acid solution was soaked and disinfected for 2 hours, and the treated Achilles tendon tissue was frozen at -45°C for 6 hours, and then it was pulverized with a homogenizer, and the pulverized pig Achilles tendon particles were mixed with 0.1wt% trypsin Extract in PBS buffer solution for 2h, extract in 0.1%EDTA PBS buffer solution for 2h, then carry out decellularization treatment in 1wt%TBP PBS buffer solution for 4h, remove cell components, and then use 100μg / ml ribonuclease and 100 μg / ml deoxyribonuclease was tre...
Embodiment 3
[0033] Example 3 Preparation of tendon repair material with bovine Achilles tendon
[0034] (1) Take the freshly slaughtered Achilles tendon under aseptic conditions, use surgical scissors to remove fat fragments and other impurities on the Achilles tendon, rinse blood stains and other dirt with PBS buffer, and then soak and disinfect with 0.6% peracetic acid solution 0.5h, freeze the treated Achilles tendon tissue at -30°C for 10h, then use a homogenizer to pulverize it, and extract the pulverized porcine Achilles tendon granules in 0.2wt% trypsin PBS buffer solution for 1h , extracted in PBS buffer solution of 0.2% EDTA for 1h, then decellularized in PBS buffer solution of 2wt%TBP for 3h to remove cell components, and then treated with 200μg / ml ribonuclease and 200μg / ml deoxyribonucleic acid The enzyme was treated with PBS buffer solution for 1 hour to remove residual nucleic acid, the pH was adjusted to the pH value of human body fluid, and washed with PBS buffer solution. ...
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