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Method for preparing tendon repair material

A technology for repairing materials and tendons, applied in the field of preparation of tendon repair materials, to achieve the effect of eliminating immunogenicity, good biocompatibility, and high clinical practical value

Inactive Publication Date: 2018-02-27
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The research on tissue-engineered tendons has opened up a new way to repair tendon injuries, but it still faces two challenges. One is how to prepare and screen repair materials with special mechanical strength and properties. The problem of rapid proliferation in vitro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of tendon repair material using porcine Achilles tendon

[0026] (1) Take freshly slaughtered pig Achilles tendon under sterile conditions, use surgical scissors to remove fat fragments and other impurities on the Achilles tendon, rinse blood stains and other dirt with PBS buffer, and then soak and disinfect with 0.5% peracetic acid solution 1h, the treated Achilles tendon tissue was frozen at -40°C for 8h, then pulverized with a homogenizer, and the pulverized porcine Achilles tendon granules were extracted in 0.15 wt% trypsin in PBS buffer solution for 1.5h , extracted in PBS buffer solution of 0.15% EDTA for 1.5h, then decellularized in PBS buffer solution of 1.5wt%TBP for 3.5h to remove cellular components, and then treated with 150μg / ml ribonuclease and 150μg / ml Deoxyribonuclease was treated with PBS buffer solution for 1.5 hours to remove residual nucleic acid, adjust the pH to the pH value of human body fluid, and wash with PBS buffer soluti...

Embodiment 2

[0029] Example 2 Preparation of tendon repair material using human Achilles tendon as material

[0030](1) According to the relevant procedures and permits, the Achilles tendon of the donor was removed according to the law, and the fat fragments and other impurities on the Achilles tendon were removed with surgical scissors under aseptic conditions, and the blood stains and other dirt were rinsed with PBS buffer, and then 0.1% filter solution was used. The oxyacetic acid solution was soaked and disinfected for 2 hours, and the treated Achilles tendon tissue was frozen at -45°C for 6 hours, and then it was pulverized with a homogenizer, and the pulverized pig Achilles tendon particles were mixed with 0.1wt% trypsin Extract in PBS buffer solution for 2h, extract in 0.1%EDTA PBS buffer solution for 2h, then carry out decellularization treatment in 1wt%TBP PBS buffer solution for 4h, remove cell components, and then use 100μg / ml ribonuclease and 100 μg / ml deoxyribonuclease was tre...

Embodiment 3

[0033] Example 3 Preparation of tendon repair material with bovine Achilles tendon

[0034] (1) Take the freshly slaughtered Achilles tendon under aseptic conditions, use surgical scissors to remove fat fragments and other impurities on the Achilles tendon, rinse blood stains and other dirt with PBS buffer, and then soak and disinfect with 0.6% peracetic acid solution 0.5h, freeze the treated Achilles tendon tissue at -30°C for 10h, then use a homogenizer to pulverize it, and extract the pulverized porcine Achilles tendon granules in 0.2wt% trypsin PBS buffer solution for 1h , extracted in PBS buffer solution of 0.2% EDTA for 1h, then decellularized in PBS buffer solution of 2wt%TBP for 3h to remove cell components, and then treated with 200μg / ml ribonuclease and 200μg / ml deoxyribonucleic acid The enzyme was treated with PBS buffer solution for 1 hour to remove residual nucleic acid, the pH was adjusted to the pH value of human body fluid, and washed with PBS buffer solution. ...

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PUM

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Abstract

The invention relates to the field of regeneration and repair of tissues and organs and in particular relates to a method for preparing a tendon repair material and provides a novel path for tendon defect repair. The tendon repair material disclosed by the invention is prepared by binding animal-derived decellularized achilles tendon material and specific tendon seed cells. The achilles tendon material selectively remains various expected beneficial components contributing to tendon regeneration while eliminating immunogenicity of heterologous tissues, while the seed cells are in-vitro controllable induced receptor tendon cells of exogenous growth factors. According to the combination of the achilles tendon material and the seed cells, an effect of regenerating tissue-engineered tendons iseffectively achieved, the clinical applicability is high, and the tendon repair material is preferentially but not limited to be applied to tendon repair.

Description

technical field [0001] The invention relates to the field of tissue and organ regeneration and repair, in particular to a preparation method of a tendon repair material. Background technique [0002] Tendon is the continuation of muscle, and its main function is to transmit the contraction force of muscle to bone to drive joint movement. Therefore, it often bears high-intensity mechanical loads, and such mechanical loads often lead to tendon damage, but the tendon itself does not have the ability to fully regenerate. Injured tendons heal slowly and tend to form scar tissue, which deteriorates the tissue structure and mechanical properties of the tendon. If it is not repaired in time, it will lead to the occurrence of tendinopathy, which may be accompanied by vascular injury, bone and joint injury and tearing, etc. In severe cases, it may leave serious sequelae and pose a great threat to the future health of the body. Tendon injuries are a recognized treatment challenge in o...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50A61L27/54
CPCA61L27/3604A61L27/3662A61L27/3691A61L27/3826A61L27/386A61L27/3895A61L27/50A61L27/54A61L2300/414A61L2430/10
Inventor 纪萍姜红任孝敏
Owner 山东隽秀生物科技股份有限公司
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