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Induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells and method

A mesenchymal stem cell and chondrogenic technology, which is applied in the field of induction system to efficiently promote chondrogenic differentiation of mesenchymal stem cells, can solve the problems of not meeting the high efficiency requirements, long induction time, etc., and achieve low cost, short culture time, and remarkable results Effect

Active Publication Date: 2019-11-19
GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the induction time is relatively long, requiring at least 28 days, which does not meet the high-efficiency requirements of industrialization

Method used

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  • Induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells and method
  • Induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells and method
  • Induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] An induction system for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising a first induction medium and a second induction medium, the first induction medium includes a growth medium and KGN, and the first induction medium includes The concentration of KGN is 1 μmol / L, and the second induction medium includes a cartilage induction differentiation medium and an additive, and the additive includes KGN.

[0038] Wherein, the growth medium comprises the following components:

[0039]

[0040]

[0041] Wherein, the chondrogenic differentiation medium is HG-DMEM medium, and the HG-DMEM medium comprises the following components:

[0042] L-glutamic acid 4mmol / L

[0043] Glucose 4500mg / L

[0044] Sodium pyruvate 0.011g / L.

[0045] The additive includes the following components:

[0046]

[0047] A method for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising the following steps:

[0048] A...

Embodiment 2

[0060] An induction system for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising a first induction medium and a second induction medium, the first induction medium includes a growth medium and KGN, and the first induction medium includes The concentration of KGN is 0.5 μmol / L, and the second induction medium includes a cartilage induction differentiation medium and an additive, and the additive includes KGN.

[0061] Wherein, the growth medium comprises the following components:

[0062]

[0063]

[0064] Wherein, the chondrogenic differentiation medium is HG-DMEM medium, and the HG-DMEM medium comprises the following components:

[0065] L-glutamic acid 2mmol / L

[0066] Glucose 3000mg / L

[0067] Sodium pyruvate 0.05g / L;

[0068] The additive includes the following components:

[0069]

[0070]

[0071] A method for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising the following steps...

Embodiment 3

[0077] An induction system for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising a first induction medium and a second induction medium, the first induction medium includes a growth medium and KGN, and the first induction medium includes The concentration of KGN is 3 μmol / L, and the second induction medium includes a cartilage induction differentiation medium and an additive, and the additive includes KGN.

[0078] Wherein, the growth medium comprises the following components:

[0079]

[0080]

[0081] Wherein, the chondrogenic differentiation medium is HG-DMEM medium, and the HG-DMEM medium comprises the following components:

[0082] L-glutamic acid 6mmol / L

[0083] Glucose 6000mg / L

[0084] Sodium pyruvate 0.15g / L;

[0085] The additive includes the following components:

[0086]

[0087] A method for efficiently promoting chondrogenic differentiation of mesenchymal stem cells, comprising the following steps:

[0088] A....

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Abstract

The invention relates to the technical field of mesenchymal stem cells, in particular to an induction system capable of efficiently promoting chondrogenic differentiation of mesenchymal stem cells. The induction system comprises a first induction culture medium and a second induction culture medium, the first induction culture medium comprises a growth culture medium and KGN, the concentration ofthe KGN in the first induction culture medium is 0.5-3 micromole / L, the second induction culture medium comprises a cartilage-induced differentiation culture medium and an additive, the additive comprises the KGN, and the concentration of the KGN in the second induction culture medium is 10-500 nmol / L. The mesenchymal stem cells are firstly pretreated through the KGN, and then induction is conducted on the cells through a low concentration KGN induction system, and mesenchymal stem cells can perform efficient differentiation on cartilage cells, good cartilage cells can be formed only within eight days, the culture time is short, the cost is low, and the system takes effect significantly.

Description

technical field [0001] The invention relates to the technical field of mesenchymal stem cells, in particular to an induction system and method for efficiently promoting chondrogenic differentiation of mesenchymal stem cells. Background technique [0002] Knee cartilage tissue is prone to damage due to trauma or disease, but articular cartilage lacks regenerative ability, and it is difficult to repair cartilage tissue after damage. Mesenchymal stem cells (Mesenchymal stem cells, MSCs) are derived from mesoderm, theoretically this type of cells can be differentiated into mesoderm tissue cells, and has a strong ability to proliferate and differentiate. Mesenchymal stem cells have a wide range of sources in the human body, such as umbilical cord, placenta, fat, bone marrow, dental pulp, umbilical cord blood, etc., and can be expanded on a large scale in vitro. Mesenchymal stem cells have a strong immune regulation function and can effectively promote the recovery of hematopoiet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0655C12N2500/30C12N2500/32C12N2500/34C12N2500/12C12N2500/38
Inventor 李相鲁吕品雷张严冬李莉黄海波
Owner GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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