Method for regenerating prunus mume plants in somatic embryo way and special culture medium of method
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A technology of somatic embryos and regenerated plants, which is applied in the field of plant tissue culture, can solve the problems of not being able to meet the genetic transformation of plum blossoms and plant regeneration, and low regeneration efficiency, and achieve good multiplication effects, efficient regeneration, and wide-ranging sources.
Pending Publication Date: 2021-08-03
BEIJING FORESTRY UNIVERSITY +1
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Problems solved by technology
[0008] Although the above-mentioned organogenesis pathway using leaves as explants and somatic embryogenesis pathways using immature zygotic embryos as explants have obtained regenerated plants, there are problems such as low regeneration efficiency, which cannot meet the requirements of plum blossom genetic transformation and plant growth. regeneration needs
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Embodiment 1
[0062] This embodiment provides a quincunx somatic embryo germination medium, its composition is as follows: 1 / 2Q&L basic medium added 6-BA 2.0mg / L, NAA 0.5mg / L, TDZ 1.0mg / L, GA 3 0.5mg / L, sucrose 30g / L, Agar 7.5g / L, pH6.0.
Embodiment 2
[0064] This embodiment provides a quincunx somatic embryo germination medium, its composition is as follows: 1 / 2Q&L basic medium added 6-BA 2.0mg / L, NAA 0.5mg / L, TDZ 1.0mg / L, GA 3 1.0mg / L, sucrose 30g / L, Agar 7.5g / L, pH6.0.
Embodiment 3
[0066] This embodiment provides a reagent for regeneration of plum blossoms through the somatic embryo pathway, which includes somatic embryo induction medium, somatic embryo proliferation medium (also can be used as somatic embryo subculture medium) and somatic embryo Germination medium.
[0067] Among them, the composition of somatic embryo induction medium is as follows: 1 / 2 MS basic medium, 6-BA0.5mg / L, 2,4-D2.0mg / L, sucrose 30g / L, GEL 2.5g / L, pH 6.0 ;
[0068] The composition of somatic embryo proliferation medium is as follows: 1 / 2 Q&L basic medium, 2,4-D 1.5mg / L, 6-BA0.5mg / L, sucrose 30g / L, Agar 7.5g / L, pH 6.0;
[0069] The composition of somatic embryo germination medium is as follows: 1 / 2Q&L basic medium supplemented with 6-BA2.0mg / L, NAA0.5mg / L, TDZ1.0mg / L, GA 3 0.5mg / L, sucrose 30g / L, Agar 7.5g / L, pH 6.0.
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Abstract
The invention relates to the technical field of plant tissue culture, in particular to a method for regenerating prunus mume plants in a somatic embryo way and a special culture medium of the method. The invention provides the method for regenerating the prunus mume plants in the somatic embryo way. The method comprises the following steps: culturing prunus mume explants on a somatic embryo induction culture medium to obtain somatic embryos, and proliferating the somatic embryos on a somatic embryo proliferation culture medium to obtain secondary somatic embryos, and culturing the secondary somatic embryos in a somatic embryo germination culture medium to form plants. According to the method, the efficiency of generating the somatic embryos through induction of explants is high, the somatic embryos can be continuously proliferated in a secondary somatic embryo form, the proliferation effect is good, long-term subculture preservation can be achieved, the ability of the somatic embryos to be differentiated into plants can be kept, and the somatic embryos can be efficiently differentiated and regenerated into prunus mume plants after being cultured through the germination culture medium.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for regenerating plum blossoms through somatic embryos and a special culture medium thereof. Background technique [0002] Plum blossom (Prunus mume) is a traditional famous flower in China, which has an important position and influence in Chinese garden and flower culture. Except China, only a few countries such as Japan, North Korea have plum blossom cultivation at present. In China, plum blossoms have a history of more than 3,000 years of application and a history of cultivation of more than 2,000 years. Research on plum blossoms has been relatively active in China. Plum blossoms are mainly distributed in the Yangtze River Basin and the south of China, roughly bounded by the Yellow River in the north and the Pearl River in the south. In northern China, plum blossom varieties are mainly apricot plum and plum plum, and true plum varieties are rare. The cu...
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