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Culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, kit containing culture medium and application of kit

A technique for dental pulp stem cells and adipogenic cells, which is applied in the field of culture medium for inducing dental pulp stem cells to differentiate into adipogenic cells, and achieves the effects of strong operability, avoiding exogenous pollution and excellent adipogenic differentiation effect.

Active Publication Date: 2022-01-28
GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing induction medium can induce the adipogenic differentiation of mesenchymal stem cells, stem cells from different sources need their own suitable induction and differentiation environment due to the differences in the microenvironment

Method used

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  • Culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, kit containing culture medium and application of kit
  • Culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, kit containing culture medium and application of kit
  • Culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, kit containing culture medium and application of kit

Examples

Experimental program
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Effect test

Embodiment 1-3 and comparative example 1-6

[0043] A culture medium for inducing dental pulp stem cells to differentiate into adipocytes, the composition of which is shown in Table 1. The preparation method specifically includes the following steps: adding 10-30 μg / mL to high-glucose DMEM medium (glucose concentration 3151mg / L) Insulin, 5-20% (volume fraction) platelet lysate, 0.1-1mM indomethacin, 0.1-1mM dexamethasone, 0.1-1mM 3-isobutyl-1-methylxanthine, 0.5-2% ( volume fraction) penicillin-streptomycin mixed solution, mixed evenly, and sterilized by filtration with a 0.22 μm filter membrane to obtain a medium for inducing dental pulp stem cells to differentiate into adipocytes.

[0044] Table 1 The components of the adipogenic induction and differentiation medium of dental pulp stem cells

[0045]

Embodiment 4

[0047] A method for inducing dental pulp stem cells to differentiate into adipocytes, specifically comprising the following steps:

[0048] (1) Take out the cryopreservation tube containing the dental pulp stem cells in the logarithmic growth phase from the liquid nitrogen at -196°C, immediately put it into a 37°C constant temperature water bath, shake it back and forth until all the ice cubes melt, and remove it from the water bath Take out the cryopreservation tube, spray the surface of the cryopreservation tube with 75% alcohol, put it into a biological safety cabinet and wipe it fully, transfer the dental pulp stem cells to a container containing 10 mL of the induced dental pulp stem cells obtained in Example 1 to differentiate into adipocytes In a 15mL centrifuge tube of the culture medium, centrifuge at 1000rpm for 5min, discard the supernatant, and resuspend the cells in the medium for inducing the differentiation of dental pulp stem cells into adipogenic cells obtained ...

Embodiment 5

[0054] A method for inducing dental pulp stem cells to differentiate into adipocytes, specifically comprising the following steps:

[0055] (1) Take out the cryopreservation tube containing the dental pulp stem cells in the logarithmic growth phase from the liquid nitrogen at -196°C, immediately put it into a 37°C constant temperature water bath, shake it back and forth until all the ice cubes melt, and remove it from the water bath Take out the cryopreservation tube, spray the surface of the cryopreservation tube with 75% alcohol, put it into a biological safety cabinet and wipe it fully, transfer the dental pulp stem cells to a container containing 10 mL of the induced dental pulp stem cells obtained in Example 2 to differentiate into adipocytes In a 15mL centrifuge tube of the culture medium, centrifuge at 1000rpm for 5min, discard the supernatant, and resuspend the cells with the culture medium for inducing the differentiation of dental pulp stem cells into adipogenic cells...

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Abstract

The invention provides a culture medium for inducing differentiation of dental pulp stem cells into adipoblasts, a kit containing the culture medium and application of the kit. The culture medium comprises a basic culture medium, insulin, a platelet lysis buffer, indometacin, dexamethasone and 3-isobutyl-1-methylxanthine. The components contained in the culture medium have a good synergistic effect, so induced differentiation from the dental pulp stem cells to the adipocytes can be achieved, the adipogenic directional differentiation specificity of the culture medium is high, the specificity of the culture medium is high, time needed for the adipogenic induced differentiation of the dental pulp stem cells can be greatly shortened, and induced differentiation efficiency is improved. Meanwhile, the culture medium is applied to the kit for inducing the differentiation of the dental pulp stem cells into the adipoblasts. The kit is used for inducing the dental pulp stem cells, and stable and efficient induced differentiation of the dental pulp stem cells into the adipoblasts can be achieved.

Description

technical field [0001] The invention relates to the technical field of stem cells, in particular to a culture medium for inducing dental pulp stem cells to differentiate into adipogenic cells, a kit containing the culture medium and applications thereof. Background technique [0002] Dental pulp stem cells (DPSCs) are a kind of mesenchymal stem cells (MSCs), which have the same good proliferation and differentiation ability and multilineage differentiation potential as other mesenchymal stem cells. [0003] The application of adipocytes in cosmetic repair of soft tissue injuries has the advantages of strong plasticity and adaptability to different defect shapes. Adipose tissue regeneration technology is an important technical means to rebuild and repair adipose tissue loss and damage caused by aging and disease factors. Adipocytes, as the seed cell source of fat regeneration engineering, can greatly drive the reconstruction of new tissue, so adipogenic differentiation It ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0653C12N5/0664C12N2501/33C12N2500/84C12N2501/39C12N2500/40C12N2501/11C12N2501/115C12N2501/999
Inventor 曾皓宇沈振波刘园月林燕纯叶凌辛嫚刘素娜郭艳正
Owner GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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