Protein chip for female infertility detection and kit thereof

A technology of female infertility and protein chip, which is applied in the field of protein chip disease detection products, can solve the problems of lagging detection indicators, lack of integration, single detection function, etc., and achieve the effect of ensuring effectiveness

Inactive Publication Date: 2010-06-23
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the shortcomings of single detection function, lagging detection indicators, and no integration of autoimmunity detection products on the market

Method used

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  • Protein chip for female infertility detection and kit thereof
  • Protein chip for female infertility detection and kit thereof
  • Protein chip for female infertility detection and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of a protein chip for female infertility detection

[0039] The main steps are: (1) Prepare the matrix nitrocellulose membrane; (2) Design the chip, determine the arrangement of the lattice and the spotting position; (3) Draw the antibody from the 384-well plate and spray it on the nitrocellulose (4) assemble a nitrocellulose membrane, and carry out sealing, drying, packaging, and preservation.

[0040] The preparation method of the above-mentioned protein chip for detecting antibodies to female infertility markers specifically includes the following steps:

[0041] 1. Determination of antigen and antibody detection of female infertility markers:

[0042] Determination of the antigen detected by the female infertility marker kit:

[0043] The antigens used to prepare the protein chip for detection of female infertility are all extracted from animal tissues or organs. OAg is from monkey ovary, EAg is from bovine endometrium, SAg is from human semen, ZAg is...

Embodiment 2

[0051] Kit preparation

[0052] A protein chip kit for infertility detection, consisting of a protein chip for infertility detection, standard products, quality control products, sample diluents, enzyme-labeled working solutions, concentrated washing solutions, and detection solutions; each part is bottled, Fix it in the box with a paper template (such as figure 2 ), not limited to the placement method, it is used together with the test. Here's how the parts are prepared:

[0053] 1. The infertility detection protein chip is a 6×8 well plate, with nitrocellulose membrane as the base, and the assembly method is shown in ZL032298900. After the completion of the appearance, see figure 1 .

[0054] 2. The standard product provides 6 bottles, each bottle is 0.25ml.

[0055] With 3% BSA and 0.05% preservatives (Proclin TM 300) of phosphate buffer (pH 7.4) configuration, wherein the final concentration of AOA, AEA, ASA, AZP, ACA, HCG-ab, ATA standard is shown in the following...

Embodiment 3

[0073] Application and detection of protein chips and kits

[0074] The application of the protein chip for detecting infertility of the present invention mainly uses the antigen-antibody-enzyme-labeled secondary antibody method to detect infertility marker antibodies in human blood. The method is to add human serum to the surface of the chip, and the antibodies in the sample are respectively combined with the corresponding antigens immobilized on the chip; after drying, add the enzyme-labeled secondary antibody to react, and wash away the unbound secondary antibody; after adding the detection solution, 1 Minutes, scan and collect the signal, the signal intensity of the sample point is proportional to the concentration of the antibody in the serum.

[0075] In the above-mentioned application of the protein chip kit for detecting infertility, the specific methods of antigen-antibody reaction and detection are as follows:

[0076] (1) The chips sprayed with antigen, positive co...

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Abstract

The present invention discloses a dedicated multi-index protein chip for female infertility detection and a kit thereof. The protein chip consists of a substrate, protein detection indexes arranged on the substrate in an array mode and a contrast coating for the detection indexes. The protein detection indexes cover ovary antigen (OAg), endometrium antigen (EAg), sperm antigen (SAg), zona pellucida antigen (ZAg), human chorionic gonadotropin antigen (HCG), trophoblast antigen (TAg) and cardiolipin antigen (CAg). The present invention overcomes the defects of the existing autoimmunological detection products on market, such as single detection function, lagged detection indexes and no integration. Besides the indexes of the product (patent application No: 200510102466.3), the protein chip also has the ACA index. The density of all indexes can be quantitatively detected, and the cross reaction of all indexes is eliminated. The present invention not only has high specificity and high sensitivity, but also has high accuracy and precision.

Description

technical field [0001] The invention relates to a protein chip disease detection product, in particular to a female infertility detection protein chip and a kit thereof. Background technique [0002] According to the prediction of the World Health Organization, in the 21st century, infertility will become the third largest disease after tumors and cardiovascular and cerebrovascular diseases. Authoritative data show that the incidence of unwanted infertility among married couples is 7% to 46%. Although there are differences in different regions, it is generally considered to be between 8% and 15%. At present, infertile patients account for 10% of the total number of married women in China, and 15% in foreign countries, and the incidence rate has an increasing trend year by year. Generally speaking, infertility factors account for 2 / 3 of women and 1 / 3 of men. [0003] Female infertility can be divided into primary and secondary in terms of time sequence. Primary refers to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/76G01N33/543G01N33/577
Inventor 穆海东汪宁梅穆宇豪刘纲顾蓉
Owner 上海裕隆生物科技有限公司
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