The invention discloses a method for preparing a polysaccharide composite film by using thermostable recombinant bacterial laccase and application thereof. The composite film is prepared by adding a carboxymethyl chitosan solution, bacterial cellulose, phenolic acids, a plasticizer and a surfactant into edible polysaccharide Curdlan suspension, and adjusting the pH value of the mixed solution to 3-5; ultrasonically degassing, adding the thermostable recombinant bacterial laccase to obtain a film forming liquid; pouring the film-forming liquid into a polytetrafluoroethylene plate, performing heat preservation reaction at 90-120 DEG C for 8-10 minutes, shaping and sterilizing, performing ultraviolet irradiation treatment for 5-10 minutes, performing hot air circulation drying treatment at 90-120 DEG C for 30-60 minutes, cooling at 4 DEG C, controlling humidity and temperature, drying, and peeling off the film to obtain a composite preservative film, namely the polysaccharide composite film. The composite film prepared by the method has high tensile strength and strong extensibility, has better antioxidant and antibacterial activities, and can effectively prolong the shelf life of fruits, vegetables and meat products.