Method for preparing polysaccharide composite film by thermally stable recombinant bacterial laccase and application

A technology of polysaccharide compounding and laccase, applied in the direction of biochemical equipment and methods, applications, chitin polysaccharide coatings, etc.

Inactive Publication Date: 2019-09-17
NANJING AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the preparation of composite membranes using Curdlan, carboxymethyl chitosan (CMCH), bacterial cellulose, and phenolic acid as substrates, and thermostable recombinant bacterial laccase as crosslinking agent.

Method used

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  • Method for preparing polysaccharide composite film by thermally stable recombinant bacterial laccase and application
  • Method for preparing polysaccharide composite film by thermally stable recombinant bacterial laccase and application
  • Method for preparing polysaccharide composite film by thermally stable recombinant bacterial laccase and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: Composite membrane preparation process

[0052] (1) Weigh 8g of Curdlan polysaccharide powder, dissolve in pure water, and after Curdlan is fully swollen, prepare 8g / 100mL of Curdlan suspension under the action of a high-speed shear;

[0053] (2) Add 1g carboxymethyl chitosan, 0.2g bacterial cellulose, and 0.1g chlorogenic acid to 50mL Curdlan suspension, and prepare suspension A under the action of a magnetic stirrer

[0054] (3) Add 0.3 g of glycerin and 0.3 g of Tween 80 to the suspension A in step (2), dilute to 100 mL with pure water, and prepare suspension B under the action of a magnetic stirrer;

[0055] (4) Adjust the pH to 4 with 2mol / L hydrochloric acid, homogenize with a homogenizer, degas with ultrasound, add 10U of heat-stable recombinant bacterial laccase (Lac) to obtain a film-forming solution;

[0056] (5) Pour the film-forming solution into a polytetrafluoroethylene plate; 60°C enzyme-catalyzed reaction for 40 minutes;

[0057] (6) Ultr...

Embodiment 2

[0060] Embodiment 2: Composite membrane preparation process

[0061] (1) Weigh 12g of Curdlan polysaccharide powder, dissolve in pure water, and after Curdlan is fully swollen, under the action of a high-speed shear, prepare 12g / 100mL of Curdlan suspension;

[0062] (2) Add 1.5g carboxymethyl chitosan, 0.4g bacterial cellulose, and 0.1g catechin to 50mL Curdlan suspension, and prepare suspension A under the action of a magnetic stirrer;

[0063] (3) Add 0.5 g of glycerin and 0.5 g of Tween 80 to the suspension A in step (2), dilute to 100 mL with pure water, and prepare suspension B under the action of a magnetic stirrer;

[0064] (4) Adjust the pH to 4 with 2mol / L hydrochloric acid, homogenize with a homogenizer, degas with ultrasound, add 15U of heat-stable recombinant bacterial laccase (Lac) to obtain a film-forming solution;

[0065] (5) Pour into a polytetrafluoroethylene plate; 60 ° C enzyme-catalyzed reaction for 40 minutes;

[0066] (6) Ultraviolet radiation steriliz...

Embodiment 3

[0069] Embodiment 3: Composite membrane preparation process

[0070] (1) Weigh 16g of Curdlan polysaccharide powder, dissolve in pure water, and after Curdlan is fully swollen, under the action of high-speed shearing machine, prepare 16g / 100mL of Curdlan suspension;

[0071] (2) Add 2g carboxymethyl chitosan, 0.6g bacterial cellulose, and 0.1g gallic acid to 50mL Curdlan suspension, and prepare suspension A under the action of a magnetic stirrer;

[0072] (3) Add 0.3 g of sorbitol and 0.3 g of Span to the suspension A in step (2), dilute to 100 mL with pure water, and prepare suspension B under the action of a magnetic stirrer;

[0073] (4) Adjust the pH to 4 with 2mol / L hydrochloric acid, homogenize with a homogenizer, degas with ultrasound, add 10U of heat-stable recombinant bacterial laccase (Lac) to obtain a film-forming solution;

[0074] (5) Pour into a polytetrafluoroethylene plate; 60 ° C enzyme-catalyzed reaction for 40 minutes;

[0075] (6) Ultraviolet radiation st...

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Abstract

The invention discloses a method for preparing a polysaccharide composite film by using thermostable recombinant bacterial laccase and application thereof. The composite film is prepared by adding a carboxymethyl chitosan solution, bacterial cellulose, phenolic acids, a plasticizer and a surfactant into edible polysaccharide Curdlan suspension, and adjusting the pH value of the mixed solution to 3-5; ultrasonically degassing, adding the thermostable recombinant bacterial laccase to obtain a film forming liquid; pouring the film-forming liquid into a polytetrafluoroethylene plate, performing heat preservation reaction at 90-120 DEG C for 8-10 minutes, shaping and sterilizing, performing ultraviolet irradiation treatment for 5-10 minutes, performing hot air circulation drying treatment at 90-120 DEG C for 30-60 minutes, cooling at 4 DEG C, controlling humidity and temperature, drying, and peeling off the film to obtain a composite preservative film, namely the polysaccharide composite film. The composite film prepared by the method has high tensile strength and strong extensibility, has better antioxidant and antibacterial activities, and can effectively prolong the shelf life of fruits, vegetables and meat products.

Description

technical field [0001] The invention belongs to the field of food industry biotechnology, and relates to a degradable polysaccharide composite film with excellent mechanical properties, excellent water vapor barrier performance, antibacterial and antioxidative functions and a preparation method thereof. Background technique [0002] Polysaccharide degradable film is a film made of animal and plant polysaccharides and microbial polysaccharides as the main raw material. It has good mechanical properties and transparency. Films prepared with polysaccharides as substrates have good application prospects. The mechanical properties, water blocking performance and stability of the membrane need to be improved. [0003] Curdlan, the Chinese name can get ran gum, also known as curdlan polysaccharide, is a kind of polyglucose derived from microorganisms, which is composed of a single D glucose molecule at the C1 and C3 positions, connected by β-1,3 glycosidic bonds. Branched linear m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L5/00C08L5/08C08L1/02C09D105/00C09D105/08C09D101/02C12N15/70C12N9/02A22C13/00
CPCA22C13/0013C08J5/18C08J2305/00C08J2401/02C08J2405/08C09D105/00C12N9/0061C12N15/70C12Y110/03002C08L5/08C08L1/02
Inventor 张充步国建张晓健
Owner NANJING AGRICULTURAL UNIVERSITY
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