Human placental nutritive layer cell line and its use

Abstract

The invention relates to human placenta trophoblastic cell series which is conserved in China Committee for Culture Collection of Microorganisms on September 22th in 2005 with CGMCC No.1461 conserving number. It includes the following steps: separating the trophoblastic cell form the placenta tissue which is pregnant for 6-7 weeks; filtering the cells with multiplication capacity; processing subunit gene transfection by telomere enzyme to gain four long life cell strains. The cell series has normal multiplication capacity and biological chemical function, active cell growth, and reaches the 210 generation. Thus it can be used as target cell model for filtering antifertility drug and curing pregnant correlative disease medicine, provide ideal vitro model for further studying trophoblastic cell, lay important foundation for studying embryo implantation and pregnant physiology and pathological mechanisms.

Description

technical field

[0001] The invention relates to a cell line, in particular to a human placental cytotrophoblast cell line and its application. Background technique

[0002] During pregnancy, the placenta is an important endocrine organ that maintains the exchange of nutrients and substances between the mother and fetus, and is an important guarantee for the success of fetal development and pregnancy maintenance. The main component cells in the placenta are trophoblast cells, including mononuclear cytotrophoblast cells (CTB) with active proliferation ability and multinucleate syncytiotrophoblast cells (STB) that undertake the main endocrine function. STB is composed of CTB through the cell membrane formed by fusion.

[0003] From the beginning to the end of pregnancy, most of the functions of the placenta are carried out by trophoblast cells. When the embryo is implanted, it relies on the identification, adhesion and invasion of the trophoblast cells and the endometrium to ...

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